1. Effects of miR-128 on the Proliferation and Apoptosis of Lung Cancer of Non-Small Cell by Regulating Reversion-Inducing Cysteine-Rich Protein with Kazal Motifs (RECK)
- Author
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Hong-Xi Yu, Hui Lin, Shuai Zhang, Hong-Wang Yan, Bo Zhang, Li-Qun Shan, and Ming-Dong Li
- Subjects
Chemistry ,Biomedical Engineering ,Reversion ,Medicine (miscellaneous) ,Bioengineering ,medicine.disease ,Kazal Motifs ,Cell biology ,Cysteine rich protein ,Apoptosis ,medicine ,Non small cell ,Lung cancer ,Biotechnology - Abstract
The study was aimed to analyse the effects of miR-128 on the proliferation and apoptosis of lung cancer cells of non-small cell by regulating RECK. The expression of miR-128 in non-small cell lung cancer (NSCLC) and its paracancerours tissues was analyzed by sequencing. miR-128 expression level in NSCLC and its paracancerours tissues was detected by qPCR. We over expressed miR-128 by mimics in A549 NSCLC cell line. Establish blank and negative control group. miR-128 and RECK expression level in each group was detected by qPCR. Use MTT assay to test the proliferation ability of each group. The apoptosis level and the level of ROS in each group were tested by hoechst 33258. The expression level of apoptosis-related protein and p38 NF- B signal pathway-related protein in each group was tested by Western blot. The results of sequencing and qPCR showed that compared with the blank control group, the expression level of miR-128 mRNA was significantly higher in the adjacent tissues than in the adjacent tissues (P < 0 05). The expression levels of miR128 mRNA and RECK mRNA in the overexpression group were significantly increased (P
- Published
- 2020