1. Volume-CLEM: a method for correlative light and electron microscopy in three dimensions
- Author
-
Ronja Schliep, Jan Hegermann, Matthias Ochs, Christian Mühlfeld, Lars Knudsen, Susanne Fassbender, and Christoph Wrede
- Subjects
0301 basic medicine ,Pulmonary and Respiratory Medicine ,Tissue clearing ,Materials science ,Physiology ,Cell Biology ,Mice, Inbred C57BL ,Mice ,03 medical and health sciences ,Fluorescent labelling ,Imaging, Three-Dimensional ,030104 developmental biology ,0302 clinical medicine ,Microscopy, Fluorescence ,Correlative light and electron microscopy ,030220 oncology & carcinogenesis ,Physiology (medical) ,Microscopy ,Microscopy, Electron, Scanning ,Fluorescence microscope ,Biophysics ,Animals ,Lung ,Volume (compression) - Abstract
Generation of three-dimensional (3D) data sets from serial sections of tissues imaged by light microscopy (LM) allows identification of rare structures by morphology or fluorescent labeling. Here, we demonstrate a workflow for correlative LM and electron microscopy (EM) from 3D LM to 3D EM, using the same sectioned material for both methods consecutively. The new approach is easy to reproduce in routine EM laboratories and applicable to a wide range of organs and research questions.
- Published
- 2019