27 results on '"Levi, Moshe"'
Search Results
2. Phosphonoformic acid blunts adaptive response of renal and intestinal Pi transport.
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Loghman-Adham, Mahmoud and Levi, Moshe
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PHOSPHONIC acids , *PHOSPHATES , *KIDNEY physiology , *BRUSH border membrane , *METABOLISM , *PHYSIOLOGY - Abstract
Assesses the effect of parenteral administration of phosphonoformic acid (PFA) on inorganic phosphate (Pi) handling. Effect of parenteral PFA on renal Pi excretion; Absorption by rat intestine; Effect of oral PFA on renal Pi excretion and plasma Pi; Effect of oral PFA on Pi transport in renal brush-border membrane vesicles (BBMV); Kinetics of effect of PFA in renal BBMV.
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- 1993
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3. Nascent shifts in renal cellular metabolism, structure, and function due to chronic empagliflozin in prediabetic mice.
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Shepard, Blythe D., Chau, Jennifer, Kurtz, Ryan, Rosenberg, Avi Z., Sarder, Pinaki, Border, Samuel P., Ginley, Brandon, Rodriguez, Olga, Albanese, Chris, Knoer, Grace, Greene, Aarenee, Souza, Aline M. A. De, Ranjit, Suman, Levi, Moshe, and Ecelbarger, Carolyn M.
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SODIUM-glucose cotransporters , *METABOLISM , *TYPE 2 diabetes , *EMPAGLIFLOZIN , *KIDNEY tubules , *GLOMERULAR filtration rate - Abstract
Sodium-glucose cotransporter, type 2 inhibitors (SGLT2i) are emerging as the gold standard for treatment of type 2 diabetes (T2D) with renal protective benefits independent of glucose lowering. We took a high-level approach to evaluate the effects of the SGLT2i, empagliflozin (EMPA) on renal metabolism and function in a prediabetic model of metabolic syndrome. Male and female 12-wk-old TallyHo (TH) mice, and their closest genetic lean strain (Swiss-Webster, SW) were treated with a high-milk-fat diet (HMFD) plus/minus EMPA (@0.01%) for 12-wk. Kidney weights and glomerular filtration rate were slightly increased by EMPA in the TH mice. Glomerular feature analysis by unsupervised clustering revealed sexually dimorphic clustering, and one unique cluster relating to EMPA. Periodic acid Schiff (PAS) positive areas, reflecting basement membranes and mesangium were slightly reduced by EMPA. Phasor-fluorescent life-time imaging (FLIM) of free-to-protein bound NADH in cortex showed a marginally greater reliance on oxidative phosphorylation with EMPA. Overall, net urine sodium, glucose, and albumin were slightly increased by EMPA. In TH, EMPA reduced the sodium phosphate cotransporter, type 2 (NaPi-2), but increased sodium hydrogen exchanger, type 3 (NHE3). These changes were absent or blunted in SW. EMPA led to changes in urine exosomal microRNA profile including, in females, enhanced levels of miRs 27a-3p, 190a-5p, and 196b-5p. Network analysis revealed "cancer pathways" and "FOXO signaling" as the major regulated pathways. Overall, EMPA treatment to prediabetic mice with limited renal disease resulted in modifications in renal metabolism, structure, and transport, which may preclude and underlie protection against kidney disease with developing T2D. NEW & NOTEWORTHY: Renal protection afforded by sodium glucose transporter, type 2 inhibitors (SGLT2i), e.g., empagliflozin (EMPA) involves complex intertwined mechanisms. Using a novel mouse model of obesity with insulin resistance, the TallyHo/Jng (TH) mouse on a high-milk-fat diet (HMFD), we found subtle changes in metabolism including altered regulation of sodium transporters that line the renal tubule. New potential epigenetic determinants of metabolic changes relating to FOXO and cancer signaling pathways were elucidated from an altered urine exosomal microRNA signature. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Farnesoid X receptor prevents neutrophil extracellular traps via reduced sphingosine-1-phosphate in chronic kidney disease.
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Jones, Bryce A., Myakala, Komuraiah, Guha, Mahilan, Davidson, Shania, Adapa, Sharmila, Santiago, Isabel Lopez, Schaffer, Isabel, Yang Yue, Allegood, Jeremy C., Cowart, L. Ashley, Wang, Xiaoxin X., Rosenberg, Avi Z., and Levi, Moshe
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FARNESOID X receptor , *CHRONIC kidney failure , *SPHINGOSINE-1-phosphate , *BIOMARKERS , *SPHINGOSINE kinase - Abstract
Farnesoid X receptor (FXR) activation reduces renal inflammation, but the underlying mechanisms remain elusive. Neutrophil extracellular traps (NETs) are webs of DNA formed when neutrophils undergo specialized programmed cell death (NETosis). The signaling lipid sphingosine-1-phosphate (S1P) stimulates NETosis via its receptor on neutrophils. Here, we identify FXR as a negative regulator of NETosis via repressing S1P signaling. We determined the effects of the FXR agonist obeticholic acid (OCA) in mouse models of adenosine phosphoribosyltransferase (APRT) deficiency and Alport syndrome, both genetic disorders that cause chronic kidney disease. Renal FXR activity is greatly reduced in both models, and FXR agonism reduces disease severity. Renal NETosis and sphingosine kinase 1 (Sphk1) expression are increased in diseased mice, and they are reduced by OCA in both models. Genetic deletion of FXR increases Sphk1 expression, and Sphk1 expression correlates with NETosis. Importantly, kidney S1P levels in Alport mice are two-fold higher than controls, and FXR agonism restores them back to baseline. Short-term inhibition of sphingosine synthesis in Alport mice with severe kidney disease reverses NETosis, establishing a causal relationship between S1P signaling and renal NETosis. Finally, extensive NETosis is present in human Alport kidney biopsies (six male, nine female), and NETosis severity correlates with clinical markers of kidney disease. This suggests the potential clinical relevance of the newly identified FXR-S1P-NETosis pathway. In summary, FXR agonism represses kidney Sphk1 expression. This inhibits renal S1P signaling, thereby reducing neutrophilic inflammation and NETosis. NEW & NOTEWORTHY Many preclinical studies have shown that the farnesoid X receptor (FXR) reduces renal inflammation, but the mechanism is poorly understood. This report identifies FXR as a novel regulator of neutrophilic inflammation and NETosis via the inhibition of sphingosine-1-phosphate signaling. Additionally, NETosis severity in human Alport kidney biopsies correlates with clinical markers of kidney disease. A better understanding of this signaling axis may lead to novel treatments that prevent renal inflammation and chronic kidney disease. [ABSTRACT FROM AUTHOR]
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- 2023
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5. Novel NaPi-2c mutations that cause mistargeting of NaPi-2c protein and uncoupling of Na-Pi cotransport cause HHRH.
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Levi, Moshe
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GENETICS , *RICKETS , *BONE diseases , *VITAMIN D deficiency , *RENAL osteodystrophy - Abstract
The author reflects on the mutations in the gene encoding of the renal proximal tubular sodium-phosphate cotransporter NaPi-2c, which is believed to be the cause of hereditary hypophosphatemic rickets with hypercalciuria (HHRH). HHRH is an autosomal recessive inherited disorder of mineral and bone metabolism. The author mentions the research conducted by Jaureguiberry et al., where it revealed novel insights on how mutations in NaPi-2c could result in HHRH.
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- 2008
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6. AJP-Cell Physiology initiates publication of the Hans H. Ussing Lecture.
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Levi, Moshe and Insel, Paul A.
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LECTURES & lecturing , *CONFERENCES & conventions - Abstract
The article provides information on a lecture to be presented in honor of Professor Hans Henriksen Ussing at the Experimental Biology meeting by Epithelial Transport Group of the American Physiological Society.
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- 2010
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7. Sacubitril/valsartan treatment has differential effects in modulating diabetic kidney disease in db/db mice and KKAy mice compared with valsartan treatment.
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Myakala, Komuraiah, Jones, Bryce A., Wang, Xiaoxin X., and Levi, Moshe
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DIABETIC nephropathies , *ANGIOTENSIN-receptor blockers , *VALSARTAN , *ENTRESTO , *TYPE 2 diabetes , *NATRIURETIC peptides - Abstract
Although renin-angiotensin blockade has shown beneficial outcomes in patients with diabetes, renal injury progresses in most of these patients. Therefore, there remains a need for new therapeutic targets in diabetic kidney disease. Enhancement of vasoactive peptides, such as natriuretic peptides, via neprilysin inhibition, has been a new approach. A first-in-class drug, sacubitril/valsartan (Sac/Val), a combination of the angiotensin II receptor blocker Val and neprilysin inhibitor prodrug Sac, has been shown to be more effective than renin-angiotensin blockade alone in the treatment of heart failure with reduced ejection fraction. In this study, we tested the effects of Sac/Val in diabetic kidney disease. We administered Sac/Val or Val to two type 2 diabetes mouse models, db/db mice or KKAy mice. After 3 mo of treatment, Sac/Val attenuated the progression of proteinuria, glomerulosclerosis, and podocyte loss in both models of diabetic mice. Val shared a similar improvement but to a lesser degree in some parameters compared with Sac/Val. Sac/Val but not Val decreased the blood glucose level in KKAy mice. Sac/Val exerted renal protection through coordinated effects on antioxidative stress and anti-inflammation. In both diabetic models, we revealed a new mechanism to cause inflammation, self-DNAactivated cGMP-AMP synthase-stimulator of interferon genes (cGAS-STING) signaling, which was activated in diabetic kidneys and prevented by Sac/Val or Val treatment. The present data suggest that Sac/Val has sufficient therapeutical potential to counter the pathophysiological effects of diabetic kidney disease, and its effectiveness could be better than Val alone. [ABSTRACT FROM AUTHOR]
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- 2021
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8. Inhibition of 5-lipoxygenase decreases renal fibrosis and progression of chronic kidney disease.
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Montford, John R., Bauer, Colin, Dobrinskikh, Evgenia, Hopp, Katharina, Levi, Moshe, Weiser-Evans, Mary, Nemenoff, Raphael, and Furgeson, Seth B.
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In inflammatory diseases, the 5-lipoxygenase (5-LO) pathway contributes to epithelial damage and fibrosis by catalyzing the production of leukotrienes (LTs). Antagonists of the 5-LO pathway are currently approved for use in patients and are well tolerated. We found that expression of 5-LO is strongly induced in three models of chronic kidney disease: unilateral ureteral obstruction (UUO), folate nephropathy, and an orthologous mouse model of polycystic kidney disease. Immunohistochemistry showed that macrophages are the dominant source of 5-LO. Zileuton, a US Food and Drug Administration-approved antagonist of 5-LO, significantly reduced fibrosis at 7 and 14 days after UUO; these findings were confirmed using a genetically modified [5-LO-associated protein-knockout (Alox5ap−/−)] mouse strain. Inhibition of 5-LO did not appear to change infiltration of leukocytes after UUO as measured by flow cytometry. However, fluorescence-lifetime imaging microscopy showed that 5-LO inhibitors reversed the glycolytic switch in renal tubular epithelial cells after UUO. Two downstream enzymes of 5-LO, LTA4 hydrolase (LTA4H) and LTC4 synthase (LTC4S), are responsible for the synthesis of LTB4 and cysteinyl LTs, respectively. Fibrosis was reduced after UUO in Ltc4s−/−, but not Lta4h−/−, mice. In contrast, using the folate nephropathy model, we found reduced fibrosis and improved renal function in both Ltc4s−/− and Lta4h−/− mice. In summary, our studies suggest that manipulation of the 5-LO pathway may represent a novel treatment approach for chronic kidney disease. [ABSTRACT FROM AUTHOR]
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- 2019
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9. Estrogen directly and specifically downregulates NaPi-IIa through the activation of both estrogen receptor isoforms (ERα and ERß) in rat kidney proximal tubule.
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Burris, Dara, Webster, Rose, Sheriff, Sulaiman, Faroqui, Rashma, Levi, Moshe, Hawse, John R., and Amlal, Hassane
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PHOSPHATE transport proteins , *KIDNEY disease treatments , *ESTROGEN receptors , *DOWNREGULATION , *HYPOPHOSPHATASIA , *PROTEIN expression , *LABORATORY rats , *KIDNEY proteins , *DIAGNOSIS - Abstract
We have previously demonstrated that estrogen (E2) downregulates phosphate transporter NaPi- IIa and causes phosphaturia and hypophosphatemia in ovariectomized rats. In the present study, we examined whether E2 directly targets NaPi-IIa in the proximal tubule (PT) and studied the respective roles of estrogen receptor isoforms (ERα and ERß) in the downregulation of NaPi-IIa using both in vivo and an in vitro expression systems. We found that estrogen specifically downregulates NaPi-IIa but not NaPi- IIc or Pit2 in the kidney cortex. Proximal tubules incubated in a "shake" suspension with E2 for 24 h exhibited a dose-dependent decrease in NaPi-IIa protein abundance. Results from OVX rats treated with specific agonists for either ERα [4,4',4";-(4-propyl-[1H]- pyrazole-1,3,5-triyl) trisphenol, PPT] or ERß [4,4',4"-(4-propyl-[1H]- pyrazole-1,3,5-triyl) trisphenol, DPN] or both (PPT + DPN), indicated that only the latter caused a sharp downregulation of NaPi-IIa, along with significant phosphaturia and hypophosphatemia. Lastly, heterologous expression studies demonstrated that estrogen downregulated NaPi-IIa only in U20S cells expressing both ERa and ERß, but not in cells expressing either receptor alone. In conclusion, these studies demonstrate that rat PT cells express both ERα and ERß and that E2 induces phosphaturia by directly and specifically targeting NaPi-IIa in the PT cells. This effect is mediated via a mechanism involving coactivation of both ERα and ERß, which likely form a functional heterodimer complex in the rat kidney proximal tubule. [ABSTRACT FROM AUTHOR]
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- 2015
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10. Na+-independent phosphate transport in Caco2BBE cells.
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Candeal, Eduardo, Caldas, Yupanqui A., Guillén, Natalia, Levi, Moshe, and Sorribas, Víctor
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PHOSPHATE transport proteins , *EPITHELIAL cells , *CYCLOHEXIMIDE , *DACTINOMYCIN , *SMALL intestine - Abstract
Pi transport in epithelia has both Na+-dependent and Na+-independent components, but so far only Na+-dependent transporters have been characterized in detail and molecularly identified. Consequently, in the present study, we initiated the characterization and analysis of intestinal Na+-independent P¡ transport using an in vitro model, Caco2BBE cells. Only Na+-independent Pi uptake was observed in these cells, and Pi uptake was dramatically increased when cells were incubated in high-Pi DMEM (4 mM) from 1 day to several days. No response to low-Pi medium was observed. The increased Pi transport was mainly caused by Vmax changes, and it was prevented by actinomycin D and cycloheximide. Pi transport in cells grown in 1 mM Pi (basal DMEM) decreased at pH > 7.5, and it was inhibited with proton ionophores. Pi transport in cells incubated with 4 mM Pi increased with alkaline pH, suggesting a preference for divalent phosphate. Pi uptake in cells in 1 mM Pi was completely inhibited only by Pi and partially inhibited by phosphonoformate, oxalate, DIDS, SITS, SO42-, HCO3-, and arsenate. This inhibition pattern suggests that more than one Pi transporter is active in cells maintained with 1 mM Pi. Phosphate transport from cells maintained at 4 mM Pi was only partially inhibited by phosphonoformate, oxalate, and arsenate. Attempts to identify the responsible transporters showed that multifunctional anion exchangers of the Slc26 family as well as members of Slc17, Slc20, and Slc37 and the P¡ exporter xenotropic and polytropic retrovirus receptor 1 are not involved. [ABSTRACT FROM AUTHOR]
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- 2014
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11. Urinary matrix metalloproteinase activities: biomarkers for plaque angiogenesis and nephropathy in diabetes.
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McKittrick, Ian B., Bogaert, Yolanda, Nadeau, Kristen, Snell-Bergeon, Janet, Hull, Amber, Tao Jiang, Xiaoxin Wang, Levi, Moshe, and Moulton, Karen S.
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METALLOPROTEINASES , *BIOMARKERS , *DIABETES complications , *ATHEROSCLEROSIS , *NEOVASCULARIZATION - Abstract
Diabetic complications of nephropathy and accelerated atherosclerosis are associated with vascular remodeling and dysregulated angiogenesis. Matrix metalloproteinases (MMP) modify extracellular matrix during vascular remodeling and are excreted in urine of patients with vascular malformation or tumor angiogenesis. We hypothesized that urinary MMP activities would be sensitive biomarkers for vascular remodeling in diabetic complications. Activities of MMP-2, MMP-9, and its complex with neutrophil gelatinase-associated lipocalin (NGAL/MMP-9) were measured by substrate gel zymography in urine from nondiabetic (ND) and type 1 diabetic (T1D) rodents that were susceptible to both T1D-induced plaque angiogenesis and nephropathy, or nephropathy alone. Additionally, these urine activities were measured in ND and T1D adolescents. Urinary MMP-9, MMP-2, and NGAL/MMP-9 activities were increased and more prevalent in TID compared with ND controls. Urinary MMP-2 activity was detected in mice with T1D induced plaque neovascularization. In nephropathy models, urinary NGAL/MMP-9 and MMP-9 activities appeared before onset of albuminutia, whereas MMP-2 was absent or delayed. Finally, urinary MMP activities were increased in adolescents with early stages of T1D. Urinary MMP activities may be sensitive, noninvasive, and clinically useful biomarkers for predicting vascular remodeling in diabetic renal and vascular complications. [ABSTRACT FROM AUTHOR]
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- 2011
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12. Differential modulation of the molecular dynamics of the type IIa and IIc sodium phosphate cotransporters by parathyroid hormone.
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Lanzano, Luca, Lei, Tim, Okamura, Kayo, Giral, Hector, Caldas, Yupanqui, Masihzadeh, Omid, Gratton, Enrico, Levi, Moshe, and Blaine, Judith
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MOLECULAR dynamics , *SODIUM phosphates , *PARATHYROID hormone , *CELL culture , *BRUSH border membrane - Abstract
The kidney is a key regulator of phosphate homeostasis. There are two predominant renal sodium phosphate cotransporters, NaPi2a and NaPi2c. Both are regulated by parathyroid hormone (PTH), which decreases the abundance of the NaPi cotransporters in the apical membrane of renal proximal tubule cells. The time course of PTH-induced removal of the two cotransporters from the apical membrane, however, is markedly different for NaPi2a compared with NaPi2c. In animals and in cell culture, PTH treatment results in almost complete removal of NaPi2a from the brush border (BB) within 1 h whereas for NaPi2c this process in not complete until 4 to 8 h after PTH treatment. The reason for this is poorly understood. We have previously shown that the unconventional myosin motor myosin VI is required for PTH-induced removal of NaPi2a from the proximal tubule BB. Here we demonstrate that myosin VI is also necessary for PTH-induced removal of NaPi2c from the apical membrane. In addition, we show that, while at baseline the two cotransporters have similar diffusion coefficients within the membrane, after PTH addition the diffusion coefficient for NaPi2a initially exceeds that for NaPi2c. Thus NaPi2c appears to remain "tethered" in the apical membrane for longer periods of time after PTH treatment, accounting, at least in part, for the difference in response times to PTH of NaPi2a versus NaPi2c. [ABSTRACT FROM AUTHOR]
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- 2011
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13. Effect of hypokalemia on renal expression of the ammonia transporter family members, Rh B Glycoprotein and Rh C Glycoprotein, in the rat kidney.
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Ki-Hwan Han, Hyun-Wook Lee, Handlogten, Mary E., Bishop, Jesse M., Levi, Moshe, Jin Kim, Verlander, Jill W., and Weiner, I. David
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HYPOKALEMIA , *ALKALOSIS , *AMMONIA , *LABORATORY rats , *GLYCOPROTEINS , *IMMUNOHISTOCHEMISTRY , *CELL membranes - Abstract
Hypokalemia is a common electrolyte disorder that increases renal ammonia metabolism and can cause the development of an acid-base disorder, metabolic alkalosis. The ammonia transporter family members, Rh B glycoprotein (Rhbg) and Rh C glycoprotein (Rhcg), are expressed in the distal nephron and collecting duct and mediate critical roles in acid-base homeostasis by facilitating ammonia secretion. In the current studies, the effect of hypokalemia on renal Rhbg and Rhcg expression was examined. Normal Sprague-Dawley rats received either K+-free or control diets for 2 wk. Rats receiving the K+-deficient diet developed hypokalemia and metabolic alkalosis associated with significant increases in both urinary ammonia excretion and urine pH. Rhcg expression increased in the outer medullary collecting duct (OMCD). In OMCD intercalated cells, hypokalemia resulted in more discrete apical Rhcg expression and a marked increase in apical plasma membrane immunolabel. In principal cells, in the OMCD, hypokalemia increased both apical and basolateral Rhcg immunolabel intensity. Cortical Rhcg expression was not detectably altered by immunohistochemistry, although there was a slight decrease in total expression by immunoblot analysis. Rhbg protein expression was decreased slightly in the cortex and not detectably altered in the outer medulla. We conclude that in rat OMCD, hypokalemia increases Rhcg expression, causes more polarized apical expression in intercalated cells, and increases both apical and basolateral expression in the principal cell. Increased plasma membrane Rhcg expression in response to hypokalemia in the rat, particularly in the OMCD, likely contributes to the increased ammonia excretion and thereby to the development of metabolic alkalosis. [ABSTRACT FROM AUTHOR]
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- 2011
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14. Vitamin D receptor agonist doxercalciferol modulates dietary fat-induced renal disease and renal lipid metabolism.
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Xiaoxin X. Wang, Tao Jiang, Yan Shen, Santamaria, Hannah, Solis, Nathaniel, Arbeeny, Cynthia, and Levi, Moshe
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VITAMIN D , *PROTEINURIA , *METABOLIC disorders , *GROWTH factors , *CARRIER proteins , *OXIDATIVE stress - Abstract
Diet-induced obesity (DIO) and insulin resistance in mice are associated with proteinuria, renal mesangial expansion, accumulation of extracellular matrix proteins, and activation of oxidative stress, proinflammatory cytokines, profibrotic growth factors, and the sterol regulatory element binding proteins, SREBP-1 and SREBP-2, that mediate increases in fatty acid and cholesterol synthesis. The purpose of the present study was to determine whether treatment of DIO mice with the vitamin D receptor (VDR) agonist doxercalciferol (1α-hydroxyvitamin D2) prevents renal disease. Our results indicate that treatment of DIO mice with the VDR agonist decreases proteinuria, podocyte injury, mesangial expansion, and extracellular matrix protein accumulation. The VDR agonist also decreases macrophage infiltration, oxidative stress, proinflammatory cytokines, and profibrotic growth factors. Furthermore, the VDR agonist also prevents the activation of the renin-angiotensin-aldosterone system including the angiotensin II type 1 receptor and the mineralocorticoid receptor. An additional novel finding of our study is that activation of VDR results in decreased accumulation of neutral lipids (triglycerides and cholesterol) and expression of adipophilin in the kidney by decreasing SREBP-1 and SREBP-2 expression and target enzymes that mediate fatty acid and cholesterol synthesis and increasing expression of the farnesoid X receptor. This study therefore demonstrates multiple novel effects of VDR activation in the kidney which prevent renal manifestations of DIO in the kidney. [ABSTRACT FROM AUTHOR]
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- 2011
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15. Shank2 redistributes with NaPilla during regulated endocytosis.
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Dobrinskikh, Evgenia, Giral, Hector, Caldas, Yupanqui A., Levi, Moshe, and Doctor, R. Brian
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IMMUNOFLUORESCENCE , *CELL physiology , *RATS , *LYSOSOMES , *ENDOCYTOSIS - Abstract
Dobrinskikh E, Giral H, Caldas YA, Levi M, Doctor RB. Shank2 redistributes with Serum phosphate levels are acutely impacted by the abundance of sodium-phosphate cotransporter ha (NaPihla) in the apical membrane of renal proximal tubule cells. PSD-95/Disks Large/Zonula Occludens (PDZ) domain- containing proteins bind NaPiLIa and likely contribute to the delivery, retention, recovery, and trafficking of NaPilla. Shank2 is a distinctive PDZ domain protein that binds NaPilla. Its role in regulating NaPilla activity, distribution, and abundance is unknown. In the present in vivo study, rats were maintained on a low-phosphate diet, and then plasma phosphate levels were acutely elevated by high-phosphate feeding to induce the recovery, endocytosis, and degradation of NaPilla. Western blot analysis of renal cortical tissue from rats given high-phosphate feed showed NaPihla and Shank2 underwent degra- dation. Quantitative immunofluorescence analyses, including mi- crovillar versus intracellular intensity ratios and intensity correlation quotients, showed that Shank2 redistributed with NaPilla during the time course of NaPilla endocytosis. Furthermore, NaPilla and Shank2 trafficked through distinct endosomal compartments (clathrin, early endosomes, lysosomes) with the same temporal pattern. These in vivo findings indicate that Shank2 is positioned to coordinate the regulated endocytic retrieval and downregulation of NaPilla in rat renal prox- imal tubule cells. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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16. PTH-induced internalization of apical membrane NaPi2a: role of actin and myosin VI.
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Blaine, Judith, Okamura, Kayo, Giral, Hector, Breusegem, Sophia, Caldas, Yupanqui, Millard, Andrew, Barry, Nicholas, and Levi, Moshe
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PARATHYROID hormone , *HOMEOSTASIS , *BRUSH border membrane , *SODIUM phosphates , *FLUORESCENCE microscopy , *KIDNEY abnormalities - Abstract
Parathyroid hormone (PTH) plays a critical role in the regulation of renal phosphorous homeostasis by altering the levels of the sodium-phosphate cotransporter NaPi2a in the brush border membrane (BBM) of renal proximal tubular cells. While details of the molecular events of PTH-induced internalization of NaPi2a are emerging, the precise events governing NaPi2a removal from brush border microvilli in response to PTH remain to be fully determined. Here we use a novel application of total internal reflection fluorescence microscopy to examine how PTH induces movement of NaPi2a out of brush border microvilli in living cells in real time. We show that a dynamic actin cytoskeleton is required for NaPi2a removal from the BBM in response to PTH. In addition, we demonstrate that a myosin motor that has previously been shown to be coregulated with NaPi2a, myosin VI, is necessary for PTH-induced removal of NaPi2a from BBM microvilli. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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17. The farnesoid X receptor modulates renal lipid metabolism and diet-induced renal inflammation, fibrosis, and proteinuria.
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Wang, Xiaoxin X., Jiang, Tao, Shen, Yan, Adorini, Luciano, Pruzanski, Mark, Gonzalez, Frank J., Scherzer, Pnina, Lewis, Linda, Miyazaki-Anzai, Shinobu, and Levi, Moshe
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LIPID metabolism , *FIBROSIS , *PROTEINURIA , *OBESITY , *CYTOKINES , *OXIDATIVE stress , *CHENODEOXYCHOLIC acid , *EXTRACELLULAR matrix proteins - Abstract
Diet-induced obesity is associated with proteinuria and glomerular disease in humans and rodents. We have shown that in mice fed a high-fat diet, increased renal expression of the transcriptional factor sterol-regulatory element binding protein-I (SREBP-1) plays a critical role in renal lipid accumulation and increases the activity of proinflammatory cytokines and profibrotic growth factors. In the current study, we have determined a key role of the farnesoid X receptor (FXR) in modulating renal SREBP-l activity, glomerular lesions, and proteinuria. We found that feeding a Western-style diet to DBAI2J mice results in proteinuria, podocyte loss, mesangial expansion, renal lipid accumulation, and increased expression of proinflammatory factors, oxidative stress, and profibrotic growth factors. Treatment of these mice with the highly selective and potent FXR-activating ligand 6-a-ethyl-chenodeoxycholic acid (INT-747) ameliorates triglyceride accumulation by modulating fatty acid synthesis and oxidation, improves proteinuria, prevents podocyte loss, mesangial expansion, accumulation of extracellular matrix proteins, and increased expression of profibrotic growth factors and fibrosis markers, and modulates inflammation and oxidative stress. Our results therefore indicate that FXR activation could represent an effective therapy for treatment of abnormal renal lipid metabolism with associated inflammation, oxidative stress, and kidney pathology in patients affected by obesity. [ABSTRACT FROM AUTHOR]
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- 2009
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18. Regulation of rat intestinal Na-dependent phosphate transporters by dietary phosphate.
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Giral, Hector, Caldas, Yupanqui, Sutherland, Eileen, Wilson, Paul, Breusegem, Sophia, Barry, Nicholas, Blaine, Judith, Tao Jiang, Wang, Xiaoxin X., and Levi, Moshe
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KIDNEY diseases , *CALCIFICATION , *PHOSPHATES , *GENE expression , *LABORATORY rats - Abstract
Hyperphosphatemia associated with chronic kidney disease is one of the factors that can promote vascular calcification, and intestinal Pi absorption is one of the pharmacological targets that prevents it. The type II Na-P, cotransporter NaPi-2b is the major transporter that mediates Pi reabsorption in the intestine. The potential role and regulation of other Na-Pi transporters remain unknown. We have identified expression of the type III Na-Pi cotransporter PiT-1 in the apical membrane of enterocytes. Na-Pi transport activity and NaPi-2b and PiT-1 proteins are mostly expressed in the duodenum and jejunum of rat small intestine; their expression is negligible in the ileum. In response to a chronic low-Pi diet, there is an adaptive response restricted to the jejunum, with increased brush border membrane (BBM) Na-Pi transport activity and NaPi-2b, but not PiT-i, protein and mRNA abundance. However, in rats acutely switched from a low- to a high-Pi diet, there is an increase in BBM Na-P, transport activity in the duodenum that is associated with an increase in BBM NaPi-2b protein abundance. Acute adaptive upregulation is restricted to the duodenum and induces an increase in serum Pi that produces a transient postprandial hyperphosphatemia. Our study, therefore, indicates that Na-Pi transport activity and NaPi-2b protein expression are differentially regulated in the duodenum vs. the jejunum and that postprandial upregulation of NaPi-2b could be a potential target for treatment of hyperphosphatemia. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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19. Differential regulation of the renal sodium-phosphate cotransporters NaPi-Ila, NaPi-IIc, and PiT-2 in dietary potassium deficiency.
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Breusegem, Sophia Y., Takahashi, Hideaki, Giral-Arnal, Hector, Wang, Xiaoxin, Jiang, Tao, Verlander, Jill W., Wilson, Paul, Miyazaki-Anzai, Shinobu, Sutherland, Eileen, Caldas, Yupanqui, Blame, Judith T., Segawa, Hiroko, Miyamoto, Ken-ichi, Barry, Nicholas P., and Levi, Moshe
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PHOSPHATES , *SODIUM cotransport systems , *BRUSH border membrane , *POTASSIUM deficiency diseases , *KIDNEYS , *LABORATORY rats , *LABORATORY mice - Abstract
Dietary potassium (K) deficiency is accompanied by phosphaturia and decreased renal brush border membrane (BBM) vesicle sodium (Na)-dependent phosphate (Pi) transport activity. Our laboratory previously showed that K deficiency in rats leads to increased abundance in the proximal tubule BBM of the apical Na-Pi cotransporter NaPi-Ila, but that the activity, diffusion, and clustering of NaPi-Ila could be modulated by the altered lipid composition of the K-deficient BBM (Zajicek HK, Wang H, Puttaparthi K, Halaihel N, Markovich D, Shayman J, Beliveau R, Wilson P, Rogers T, Levi M. Kidney mt 60: 694-704, 2001; Inoue M, Digman MA, Cheng M, Breusegem SY, Halaihel N, Sorribas V. Mantulin WW, Gratton E, Barry NP, Levi M. J Biol Chem 279: 49160-49171, 2004). Here we investigated the role of the renal Na-P cotransporters NaPi-lIc and PiT-2 in K deficiency. Using Western blotting, immunofluorescence, and quantitative real-time PCR, we found that, in rats and in mice, K deficiency is associated with a dramatic decrease in the NaPi-lIc protein abundance in proximal tubular BBM and in NaPi-lIc mRNA. In addition, we documented the presence of a third Na-coupled P, transporter in the renal BBM, PiT-2, whose abundance is also decreased by dietary K deficiency in rats and in mice. Finally, electron microscopy showed subcellular redistribution of NaPi-lIc in K deficiency: in control rats, NaPi-lIc immunolabel was primarily in BBM microvilli, whereas, in K-deficient rats, NaPiTIc BBM label was reduced, and immunolabel was prevalent in cytoplasmic vesicles. In summary, our results demonstrate that decreases in BBM abundance of the phosphate transporter NaPi-lIc and also PiT-2 might contribute to the phosphaturia of dietary K deficiency, and that the three renal BBM phosphate transporters characterized so far can be differentially regulated by dietary perturbations. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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20. The Na[sup+]-P[subi] cotransporter PiT-2 (SLC2OA2) is expressed in the apical membrane of rat renal proximal tubules and regulated by dietary P[subi].
- Author
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Villa-Bellosta, Ricardo, Ravera, Silvia, Sorribas, Victor, Stange, Gerti, Levi, Moshe, Murer, Heini, Biber, Jürg, and Forster, Ian C.
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BRUSH border membrane , *PHOSPHATES , *KIDNEY tubules , *IMMUNOHISTOCHEMISTRY , *WESTERN immunoblotting , *LABORATORY rats , *PHYSIOLOGY - Abstract
The Na[sup+]-P[subi] cotransporter PiT-2 (SLC2OA2) is expressed in the apical membrane of rat renal proximal tubules and regulated by dietary P,. Am J Physiol Renal Physiol 296: F691-F699, 2009. First published December 10, 2008; doi: 10.11 52/ajprenal.90623.2008.-The principal mediators of renal phosphate (P[subi]) reabsorption are the SLC34 family proteins NaPi-IIa and NaPi-IIc, localized to the proximal tubule (PT) apical membrane. Their abundance is regulated by circulatory factors and dietary P[subi] Although their physiological importance has been confirmed in knockout animal studies, significant P[subi] reabsorptive capacity remains, which suggests the involvement of other secondary-active P transporters along the nephron. Here we show that a member of the SLC20 gene family (PiT-2) is localized to the brush-border membrane (BBM) of the PT epithelia and that its abundance, confirmed by Western blot and immunohistochemistry of rat kidney slices, is regulated by dietary P[subi] In rats treated chronically on a high-P1 (1.2%) diet, there was a marked decrease in the apparent abundance of PiT-2 protein in kidney slices compared with those from rats kept on a chronic low-P1 (0.1%) diet. In Western blots of BBM from rats that were switched from a chronic lowto high-P, diet, NaPi-Ila showed rapid downregulation after 2 h; PiT-2 was also significantly downregulated at 24 h and NaPi-lic after 48 h. For the converse dietary regime, NaPi-Ila showed adaptation within 8 h, whereas PiT-2 and NaPi-lic showed a slower adaptive trend. Our findings suggest that PiT-2, until now considered as a ubiquitously expressed P housekeeping transporter, is a novel mediator of P[subi] reabsorption in the PT under conditions of acute P1 deprivation, but with a different adaptive time course from NaPi-Ila and NaPi-lIc. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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21. Interaction of MAP17 with NHERF3/4 induces translocation of the renal Na/Pi ha transporter to the trans-Golgi.
- Author
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Lanaspa, Miguel A., Girai, Héctor, Breusegem, Sophia Y., Halaíhel, Nabil, Baile, Goretti, Catalán, Julia, Carrodeguas, José A., Barry, Nicholas P., Levi, Moshe, and Sorribas, Victor
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GENE transfection , *PHOSPHATES , *CHROMOSOMAL translocation , *NEUROTRANSMITTERS , *PROTEIN kinases - Abstract
The function of the NaPiIIa renal sodium-phosphate transporter is regulated through a complex network of interacting proteins. Several PDZ domain-containing proteins interact with its COOH terminus while the small membrane protein MAP17 interacts with its NH2 end. To elucidate the function of MAP17, we identified its interacting proteins using both bacterial and mammalian two-hybrid systems. Several PDZ domain-containing proteins, including the four NHERF proteins, as well as NaPiIIa and NHE3, were found to bind to MAP17. The interactions of MAP17 with the NHERF proteins and with NaPiIIa were further analyzed in opossum kidney (OK) cells. Expression of MAP17 alone had no effect on the NaPiIIa apical membrane distribution, but coexpression of MAP17 and NHERF3 or NHERF4 induced internalization of NaPiIIa, MAP17, and the PDZ protein to the trans-Golgi network (TGN). This effect was not observed when MAP17 was cotransfected with NHERF1/2 proteins. Inhibition of protein kinase C (PKC) prevented expression of the three proteins in the TGN. Activation of PKC in OK cells transfected only with MAP17 induced complete degradation of MAPI7 and NaPiIIa. When lysosomal degradation was prevented, both proteins accumulated in the TGN. When the dopamine D1-like receptor was activated with fenoldopam, both NaPiIIa and MAP17 also accumulated in the TGN. Finally, cotransfection of MAP 17 and NHERF3 prevented the adaptive upregulation of phosphate transport activity in OK cells in response to low extracellular phosphate. Therefore, the interaction between MAP17, NHERF3/4, and NaPiIIa in the TGN could be an important intermediate or alternate path in the internalization of NaPiIIa. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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22. Shank2E binds NaPi cotransporter at the apical membrane of proximal tubule cells.
- Author
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McWilliams, Ryan R., Breusegem, Sophia Y., Brodsky, Kelley F., Eunjoon Kim, Levi, Moshe, and Doctor, R. Brian
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- *
KIDNEY tubules , *CELLS , *PROTEINS , *BIOMOLECULES , *EPITHELIAL cells , *CELL physiology - Abstract
Proteins expressing postsynaptic density (PSD)-95/Drosophila disk large (Dlg)/zonula occludens-1 (ZO-1) (PDZ) domains are commonly involved in moderating receptor, channel, and transporter activities at the plasma membrane in a variety of cell types. At the apical membrane of renal proximal tubules (PT), the type IIa NaPi cotransporter (NaPi-IIa) binds specific PDZ domain proteins. Shank2E is a spliceoform of a family of PDZ proteins that is concentrated at the apical domain of liver and pancreatic epithelial cell types and is expressed in kidney. In the present study, immunoblotting of enriched plasma membrane fractions and immunohistology found Shank2E concentrated at the brush border membrane of rat PT cells. Confocal localization of Flag-Shank2E and enhanced green fluorescent protein-NaPi-IIa in cotransfected OK cells showed these proteins colocalized in the apical microvilli of this PT cell model. Shank2E coimmunoprecipitated with NaPi-IIa from rat renal cortex tissue and HA-NaPiIIa coprecipitated with Flag-Shank2E in cotransfected human embryonic kidney HEK cells. Domain analysis showed that the PDZ domain of Shank2E specifically bound NaPi-IIa and truncation of the COOH-terminal TRL motif from NaPi-IIa abolished this binding, and Far Western blotting showed that the Shank2E- NaPi-IIa interaction occurred directly between the two proteins. NaPi-IIa activity is regulated by moderating its abundance in the apical membrane. High-Pi conditions induce NaPi-IIa internalization and degradation. In both rat kidney PT cells and OK cells, shifting to high-Pi conditions induced an acute internal redistribution of Shank2E and, in OK cells, a significant degree of degradation. In sum, Shank2E is concentrated in the apical domain of renal PT cells, specifically binds NaPi-IIa via PDZ interactions, and undergoes Pi-induced internalization. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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23. Acute and chronic changes in cholesterol modulate Na-Pi cotransport activity in OK cells.
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Breusegem, Sophia Y., Halaihel, Nabil, Inoue, Makoto, Zajicek, Hubert, Lederer, Eleanor, Barry, Nicholas P., Sorribas, Victor, and Levi, Moshe
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CHOLESTEROL , *CELLULAR mechanics , *KIDNEY diseases , *FLUORESCENCE spectroscopy , *LIPIDS - Abstract
We previously showed an inverse correlation between membrane cholesterol content and Na-Pi cotransport activity during the aging process and adaptation to alterations in dietary Pi in the rat (Levi M, Jameson DM, and van der Meer BW. Am J Physiol Renal Fluid Electrolyte Physiol 256: F85-F94, 1989). The purpose of the present study was to determine whether alterations in cholesterol content per se modulate Na-Pi cotransport activity and apical membrane Na-Pi protein expression in opossum kidney (OK) cells. Acute cholesterol depletion achieved with β-methyl cyclodextrin (β-MCD) resulted in a significant increase in Na-Pi cotransport activity accompanied by a moderate increase in apical membrane Na-Pi protein abundance and no alteration of total cellular Na-Pi protein abundance. Conversely, acute cholesterol enrichment achieved with β-MCD/cholesterol resulted in a significant decrease in Na-Pi cotransport activity with a moderate decrease in apical membrane Na-Pi protein abundance and no change of the total cellular Na-Pi protein abundance. In contrast, chronic cholesterol depletion, achieved by growing cells in lipoprotein-deficient serum (LPDS), resulted in parallel and significant increases in Na-Pi cotransport activity and apical membrane and total cellular Na-Pi protein abundance. Cholesterol depletion also resulted in a significant increase in membrane lipid fluidity and alterations in lipid microdomains as determined by laurdan fluorescence spectroscopy and imaging. Chronic cholesterol enrichment, achieved by growing cells in LPDS followed by loading with low-density lipoprotein, resulted in parallel and significant decreases in Na-Pi cotransport activity and apical membrane and total cellular Na-Pi protein abundance. Our results indicate that in OK cells acute and chronic alterations in cholesterol content per se modulate Na-Pi cotransport activity by diverse mechanisms that also include significant interactions of Na-Pi protein with lipid microdomains. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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24. Modulation of carbohydrate response element-binding protein gene expression in 3T3-L1 adipocytes and rat adipose tissue.
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Zhibin He, Tao Jiang, Zhuowei Wang, Levi, Moshe, and Jinping Li
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ADIPOSE tissues , *WILLIAMS syndrome , *CARBOHYDRATES , *FATTY acids , *INSULIN , *DIABETES - Abstract
Carbohydrate response element-binding protein (ChREBP) is a rat homolog of human Williams-Beuren syndrome region 14 and a member of the basic helix-loop-helix leucine zipper transcription factor family. Its activation was found to be inducible by carbohydrate in the liver nuclear extracts from rats fed a high-sucrose diet. ChREBP is able to bind to the carbohydrate response element on the promoter of L-type pyruvate kinase and initiate the gene transcription. The detailed expression profile and transcriptional regulation of the ChREBP gene in adipocytes have not been characterized. In the present study, we provide evidence showing that 1) the ChREBP gene is expressed in differentiated 3T3-L1 adipocytes and rat adipose tissue; 2) insulin, glucose, and the antidiabetic agent troglitazone can significantly upregulate the gene expression of ChREBP in 3T3-L1 adipocytes, whereas free fatty acids suppress its expression in this cell type; 3) fasting followed by refeeding with a high-carbohydrate diet resulted in a 10-fold increase of ChREBP mRNA level in rat adipose tissue; and 4) ChREBP expression in adipose tissue is not significantly affected by the diabetic state. Taken together, the results we present are consistent with the idea that ChREBP is an important modulator of adipocyte biology and that its expression in adipose tissue is subject to combined regulation by glucose and insulin in vivo. The induction of ChREBP may serve as a novel pharmacological pathway for troglitazone-mediated hypoglycemic effects in vivo. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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25. Regulation of renal NaPi-2 expression and tubular phosphate reabsorption by growth hormone in the juvenile rat.
- Author
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Woda, Craig B., Halaihel, Nabil, Wilson, Paul V., Haramati, Aviad, Levi, Moshe, and Mulroney, Susan E.
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PHOSPHORUS metabolism , *PARATHYROID hormone , *SOMATOSTATIN , *IMMUNOFLUORESCENCE , *LABORATORY rats - Abstract
Growth hormone (GH) is an important factor in the developmental adaptation to enhance Pi reabsorption; however, the nephron sites and mechanisms by which GH regulates renal Pi uptake remain unclear and are the focus of the present study. Micropuncture experiments were performed after acute thyroparathyroidectomy in the presence and absence of parathyroid hormone (PTH) in adult (14- to 17-wk old), juvenile (4-wk old), and GH-suppressed juvenile male rats. While the phosphaturic effect of PTH was blunted in the juvenile rat compared with the adult, suppression of GH in the juvenile restored fractional Pi excretion to adult levels. In the presence or absence of PTH, GH suppression in the juvenile rat caused a significant increase in the fractional Pi delivery to the late proximal convoluted (PCT) and early distal tubule, so that delivery was not different from that in adults. These data were confirmed by Pi uptake studies into brush-border membrane (BBM) vesicles. Immunofluorescence studies indicate increased BBM type IIa NaPi cotransporter (NaPi-2) expression in the juvenile compared with adult rat, and GH suppression reduced NaPi-2 expression to levels observed in the adult. GH replacement in the [N-acetyl-Tyr1-D-Arg2]-GRF-(1–29)-NH2-treated juveniles restored high NaPi-2 expression and P1 uptake. Together, these novel results demonstrate that the presence of GH in the juvenile animal is crucial for the early developmental upregulation of BBM NaPi-2 and, most importantly, describe the enhanced Pi reabsorption along the PCT and proximal straight nephron segments in the juvenile rat. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
26. Central control of renal sodium-phosphate (NaPi-2) transporters.
- Author
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Mulroney, Susan E., Woda, Craig B., Halaihel, Nabil, Louie, Brandon, McDonnell, Kevin, Schulkin, Jay, Haramati, Aviad, and Levi, Moshe
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- *
SODIUM phosphates , *PHOSPHORUS in the body , *LABORATORY rats , *KIDNEYS , *BIOLOGICAL transport , *PHYSIOLOGICAL control systems - Abstract
Regulation of phosphate (Pi) reabsorption occurs through the up- and downregulation of the renal type-II sodium Pi cotransporters (NaPi-2). Recently, renal NaPi2-type expression has been identified in areas of the brain. The present study determined whether brain NaPi-2 is regulated by dietary Pi and whether the behavioral and renal adaptations to low-dietary Pi are controlled centrally. NaPi-2-1ike expression in the third ventricle (3V) and amygdala of juvenile Wistar rats was regulated by dietary Pi, as in the kidneys. When cerebrospinal fluid (CSF) Pi concentration was elevated by 3V injections of Pi in rats fed low-Pi diet (LPD), the behavioral and renal adaptations to LPD were abolished. Most importantly, NaPi-2 expression was markedly reduced not only in the brain, but also renal proximal tubules, despite the low plasma Pi milieu. This was confirmed by the significant reduction in the transport maximum for Pi (from 8.1 ± 0.2 in LPD + veh 3V to 1.7 ± 0.1 µmol Pi/ml glomerular filtration rate in LPD + 3V Pi, P < 0.001). These findings indicate that NaPi-2-1ike transporters in the brain are regulated by both dietary Pi and CSF Pi concentrations, and most significantly, that the central Pi milieu can regulate renal NaPi-2 expression. We hypothesize that central 3V NaPi-2 transporters may act as Pi sensors and help regulate both brain and whole body Pi homeostasis. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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27. Renal tubular sites of increased phosphate transport and NaPi-2 expression in the juvenile rat.
- Author
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Woda, Craig, Mulroney, Susan E., Halaihel, Nabil, Sun, Lijun, Wilson, Paul V., Levi, Moshe, and Haramati, Aviad
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- *
KIDNEY tubules , *PHOSPHATES , *ABSORPTION - Abstract
Presents information on a study which determined the tubular sites and mechanisms involved in renal phosphate reabsorption. Methodology; Results of the study; Discussion.
- Published
- 2001
- Full Text
- View/download PDF
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