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Your search keyword '"Garcia-Rill, Edgar"' showing total 9 results
Start Over Author "Garcia-Rill, Edgar" Publisher american physiological society
9 results on '"Garcia-Rill, Edgar"'

9. Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implications.

Author

Urbano FJ, Bisagno V, and Garcia-Rill E

Subjects
Actin Cytoskeleton metabolism, Animals, Calcium metabolism, Calcium Channels metabolism, Epigenesis, Genetic genetics, Female, Male, Membrane Potentials physiology, Rats, Rats, Sprague-Dawley, Actins metabolism, Epigenesis, Genetic physiology, Neurons metabolism, Pedunculopontine Tegmental Nucleus metabolism
Abstract

The pedunculopontine nucleus (PPN) is part of the reticular activating system (RAS) in charge of arousal and rapid eye movement sleep. The presence of high-frequency membrane oscillations in the gamma-band range in the PPN has been extensively demonstrated both in vivo and in vitro. Our group previously described histone deacetylation (HDAC) inhibition in vitro induced protein changes in F-actin cytoskeleton and intracellular Ca 2+ concentration regulation proteins in the PPN. Here, we present evidence that supports the presence of a fine balance between HDAC function and calcium calmodulin kinase II-F-actin interactions in the PPN. We modified F-actin polymerization in vitro by using jasplakinolide (1 μM, a promoter of F-actin stabilization), or latrunculin-B (1 μM, an inhibitor of actin polymerization). Our results showed that shifting the balance in either direction significantly reduced PPN gamma oscillation as well as voltage-dependent calcium currents.

Published
2020
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