1. High-protein-low-carbohydrate diet: deleterious metabolic and cardiovascular effects depend on age
- Author
-
Charles-Henry Cottart, Jean-Louis Paul, Tatiana Bedarida, Francoise Vibert, Valérie Nivet-Antoine, Jean-Louis Beaudeux, Jean-Louis Golmard, Florence Noble, Carmen Marchiol-Fournigault, Audrey Ayer, Daniel Henrion, Stéphanie Baron, Emilie Vessières, Biologie Neurovasculaire et Mitochondriale Intégrée (BNMI), and Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Blood Glucose ,Carbohydrate-Restricted/adverse effects ,Physiology ,[SDV]Life Sciences [q-bio] ,Inbred C57BL ,medicine.disease_cause ,Diet, Carbohydrate-Restricted ,Ventricular Dysfunction, Left ,Mice ,chemistry.chemical_compound ,Ventricular Dysfunction ,echocardiography ,Resistin ,Mesenteric arteries ,Aorta ,Resistin/blood ,Age Factors ,Mesenteric Arteries ,3. Good health ,Triglycerides/blood ,Myocardium/metabolism/pathology ,medicine.anatomical_structure ,Aorta/metabolism/pathology ,Dietary Proteins ,Cardiology and Cardiovascular Medicine ,Left/etiology/metabolism ,medicine.medical_specialty ,Biology ,Carbohydrate metabolism ,Mesenteric Arteries/metabolism/pathology ,Physiology (medical) ,medicine.artery ,Internal medicine ,Glucose Intolerance ,medicine ,Animals ,Triglycerides ,Blood Glucose/metabolism ,Triglyceride ,Myocardium ,Lipid metabolism ,Metabolism ,Glucose Intolerance/etiology/metabolism ,Lipid Metabolism ,Dietary Proteins/administration and dosage/adverse effects ,Diet ,Mice, Inbred C57BL ,Endocrinology ,chemistry ,Oxidative stress - Abstract
International audience; High-protein-low-carbohydrate (HP-LC) diets have become widespread. Yet their deleterious consequences, especially on glucose metabolism and arteries, have already been underlined. Our previous study (2) has already shown glucose intolerance with major arterial dysfunction in very old mice subjected to an HP-LC diet. The hypothesis of this work was that this diet had an age-dependent deleterious metabolic and cardiovascular outcome. Two groups of mice, young and adult (3 and 6 mo old), were subjected for 12 wk to a standard or to an HP-LC diet. Glucose and lipid metabolism was studied. The cardiovascular system was explored from the functional stage with Doppler-echography to the molecular stage (arterial reactivity, mRNA, immunohistochemistry). Young mice did not exhibit any significant metabolic modification, whereas adult mice presented marked glucose intolerance associated with an increase in resistin and triglyceride levels. These metabolic disturbances were responsible for cardiovascular damages only in adult mice, with decreased aortic distensibility and left ventricle dysfunction. These seemed to be the consequence of arterial dysfunctions. Mesenteric arteries were the worst affected with a major oxidative stress, whereas aorta function seemed to be maintained with an appreciable role of cyclooxygenase-2 to preserve endothelial function. This study highlights for the first time the age-dependent deleterious effects of an HP-LC diet on metabolism, with glucose intolerance and lipid disorders and vascular (especially microvessels) and cardiac functions. This work shows that HP-LC lead to equivalent cardiovascular alterations, as observed in very old age, and underlines the danger of such diet.
- Published
- 2014