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An instrument-free particle-templated droplet formation can be achieved upon simple mixing of amphiphilic particles with aqueous and oil phases in a well plate by using a common lab pipette. Here, a two-dimensional, two-phase flow model was established using a finite element method to mimic the droplet formation within a concentric amphiphilic particle, which consisted of an outer hydrophobic layer and an inner hydrophilic layer. Immiscible water and oil phases selectively interacted with the hydrophilic and hydrophobic layers of the particle, respectively, to form an isolated aqueous compartment within a cavity. Three extreme models were also simulated, including completely hydrophilic, completely hydrophobic, and oppositely amphiphilic particle, which indicated that a right order of the particle layers was necessary to capture the droplet inside the cavity. Moreover, we performed a systematic study of particle-templated droplet formation by varying the individual layer thicknesses of particle, particle height, interfacial tension between water and oil, contact angle of interface with different surfaces, velocity of incoming oil media, and distance between neighboring particles. The volume fraction of water droplet trapped within the target cavity region was calculated to characterize the droplet formation. Our work will help to optimize the particle fabrication process, predict the experiment droplet formation, and explain the physical mechanism underlying compartmentalization phenomena. [ABSTRACT FROM AUTHOR]

The realization of efficient mixing of samples inside a microfluidic channel is essential for performing numerous biological assays in miniaturized total analysis systems. The low Reynolds number flows at the microscale create laminar streams inside the microchannel, limiting flow mixing to a molecular diffusion level. In this paper, we propose a simple and efficient acoustofluidic mixing technique inside a single-layered polydimethylsiloxane (PDMS) microfluidic channel. The proposed surface acoustic wave (SAW)-based system composed of a straight interdigitated transducer (IDT) is positioned beneath the PDMS microchannel. Fluorescein dye dissolved in deionized water (sample fluid) and deionized water (sheath fluid) was introduced through the first and second inlets of the PDMS microchannel, respectively. Their flow rates were controlled such that the sample fluid with fluorescein dye was hydrodynamically focused close to the bottom of the microchannel by the sheath fluid. High-frequency (140 MHz) SAWs, generated from the IDT placed right beneath the first outlet, mixed the two fluids under the influence of strong acoustic streaming flows. The mixed samples were then collected at the two outlet ports for further analysis of the mixing efficiency. The developed acoustofluidic mixing device required an input voltage of 12 Vpp at a total flow rate of 50 μl/min to realize complete mixing. At a similar applied voltage, the throughput of the proposed device could be further increased to 200 μl/min with a mixing efficiency of >90%. [ABSTRACT FROM AUTHOR]

Droplets in microfluidic systems can contain microscale objects such as cells and microparticles. The control of the positions of microscale objects within a microchannel is crucial for practical applications in not only continuous-flow-based but also droplet-based systems. This paper proposes an active method for the separation of microparticles inside moving droplets which uses travelling surface acoustic waves (TSAWs). We demonstrate the preconcentration and separation of 5 and 10 lm polystyrene microparticles in moving water-in-oil droplets through the application of TSAWs with two different frequencies. The microparticles inside the droplets are affected by the acoustic radiation force induced by the TSAWs to move laterally in the direction of the TSAW propagation and are thereby separated according to their size. In-droplet separation is then demonstrated through droplet splitting at a Y-junction. Compared to our previous studies, this acoustic approach offers the label-free and on-demand separation of different-sized micro-objects in moving droplets. The present method has potential uses such as in-droplet sample purification and enrichment. [ABSTRACT FROM AUTHOR]

We demonstrate a simple device to generate chemical concentration gradients in a microfluidic channel using focused travelling surface acoustic waves (F-TSAW). A pair of curved interdigitated metal electrodes deposited on the surface of a piezoelectric (LiNbO3) substrate disseminate high frequency sound waves when actuated by an alternating current source. The F-TSAW produces chaotic acoustic streaming flow upon its interaction with the fluid inside a microfluidic channel, which mixes confluent streams of chemicals in a controlled fashion for an adjustable and rapidly switching gradient generation. [ABSTRACT FROM AUTHOR]

Surface acoustic waves (SAWs) generated in a piezoelectric substrate couple with a liquid according to Snell's law such that a compressional acoustic wave propagates obliquely at a Rayleigh angle ( θ t ) inside the microchannel to form a region devoid of a direct acoustic field, which is termed a microchannel anechoic corner (MAC). In the present study, we used microchannels with various heights and widths to characterize the width of the MAC region formed by a single travelling SAW. The attenuation of high-frequency SAWs produced a strong acoustic streaming flow that moved the particles in and out of the MAC region, whereas reflections of the acoustic waves within the microchannel resulted in standing acoustic waves that trapped particles at acoustic pressure nodes located within or outside of the MAC region. A range of actuation frequencies and particle diameters were used to investigate the effects of the acoustic streaming flow and the direct acoustic radiation forces by the travelling as well as standing waves on the particle motion with respect to the MAC region. The width of the MAC ( w c ), measured experimentally by tracing the particles, increased with the height of the microchannel ( h m ) according to a simple trigonometric equation w c = h m × tan ( θ t ). [ABSTRACT FROM AUTHOR]

Droplets in microfluidic systems can contain microscale objects such as cells and microparticles. The control of the positions of microscale objects within a microchannel is crucial for practical applications in not only continuous-flow-based but also droplet-based systems. This paper proposes an active method for the separation of microparticles inside moving droplets which uses travelling surface acoustic waves (TSAWs). We demonstrate the preconcentration and separation of 5 and 10  μ m polystyrene microparticles in moving water-in-oil droplets through the application of TSAWs with two different frequencies. The microparticles inside the droplets are affected by the acoustic radiation force induced by the TSAWs to move laterally in the direction of the TSAW propagation and are thereby separated according to their size. In-droplet separation is then demonstrated through droplet splitting at a Y-junction. Compared to our previous studies, this acoustic approach offers the label-free and on-demand separation of different-sized micro-objects in moving droplets. The present method has potential uses such as in-droplet sample purification and enrichment.