1. Construction of Epidermal Growth Factor Receptor Peptide Magnetic Nanovesicles with Lipid Bilayers for Enhanced Capture of Liver Cancer Circulating Tumor Cells
- Author
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Ying Lu, Xiaofei Liang, Kai Wang, Wenjie Tang, Youzhen Wei, Zonghai Li, Dan Li, and Jian Ding
- Subjects
Lipid Bilayers ,Cell ,Peptide ,Cell Separation ,02 engineering and technology ,Analytical Chemistry ,03 medical and health sciences ,0302 clinical medicine ,Circulating tumor cell ,Nanocapsules ,Cell Line, Tumor ,medicine ,Peptide amphiphile ,Humans ,Epidermal growth factor receptor ,Lipid bilayer ,chemistry.chemical_classification ,biology ,Chemistry ,Vesicle ,Liver Neoplasms ,Neoplastic Cells, Circulating ,021001 nanoscience & nanotechnology ,medicine.disease ,Molecular biology ,Cell biology ,ErbB Receptors ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Magnets ,biology.protein ,Peptides ,0210 nano-technology ,Liver cancer - Abstract
Highly effective targeted tumor recognition via vectors is crucial for cancer detection. In contrast to antibodies and proteins, peptides are direct targeting ligands with a low molecular weight. In the present study, a peptide magnetic nanovector platform containing a lipid bilayer was designed using a peptide amphiphile (PA) as a skeleton material in a controlled manner without surface modification. Fluorescein isothiocyanate-labeled epidermal growth factor receptor (EGFR) peptide nanoparticles (NPs) could specifically bind to EGFR-positive liver tumor cells. EGFR peptide magnetic vesicles (EPMVs) could efficiently recognize and separate hepatoma carcinoma cells from cell solutions and treated blood samples (ratio of magnetic EPMVs versus anti-EpCAM NPs: 3.5 ± 0.29). Analysis of the circulating tumor cell (CTC) count in blood samples from 32 patients with liver cancer showed that EPMVs could be effectively applied for CTC capture. Thus, this nanoscale, targeted cargo-packaging technology may be useful for designing cancer diagnostic systems.
- Published
- 2016