1. Determination of Residues of Cyromazine and Its Metabolite, Melamine, in Animal-Derived Food by Gas Chromatography−Mass Spectrometry with Derivatization
- Author
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Qian Xu, Shixin Xu, Huilan Chen, Shuhuai Wang, Xinle Zhu, and Qi Liu
- Subjects
Detection limit ,Internal standard ,Meat ,Chromatography ,Triazines ,Eggs ,Metabolite ,Pesticide Residues ,Food Contamination ,General Chemistry ,Cyromazine ,Gas Chromatography-Mass Spectrometry ,chemistry.chemical_compound ,Milk ,chemistry ,Limit of Detection ,Animals ,Cattle ,Gas chromatography ,Gas chromatography–mass spectrometry ,General Agricultural and Biological Sciences ,Derivatization ,Melamine ,Chickens ,Tilapia - Abstract
A gas chromatography-mass spectrometric (GC-MS) method was established for the determination of cyromazine and its metabolite, melamine, in animal-derived food. Chicken and tilapia muscle samples were spiked with (15)N(3)-melamine, extracted with an acidic acetonitrile/water solution, and defatted with dichloromethane. Egg and milk samples were directly extracted with 3% trichloroacetic acid. The extracts were purified using mixed cation-exchange cartridges, derived with N,O-bis(trimethylsilyl)trifluoroacetamide, and detected by GC-MS. Cyromazine and melamine were quantified by external standard methods except for the determination of melamine in animal muscle, which used an internal standard method. Recoveries ranged from 75.0 to 110.0%, and relative standard deviations were
- Published
- 2009
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