Search

Your search keyword '"Reisch, Andreas"' showing total 20 results
Start Over Author "Reisch, Andreas" Publisher american chemical society (acs)
20 results on '"Reisch, Andreas"'

3. Amplified Fluorescence in Situ Hybridization by Small and Bright Dye-Loaded Polymeric Nanoparticles

Author

Egloff, Sylvie, Melnychuk, Nina, Cruz Da Silva, Elisabete, Reisch, Andreas, Martin, Sophie, Klymchenko, Andrey S., Laboratoire de Bioimagerie et Pathologies (LBP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), European Project: 648528,H2020,ERC-2014-CoG,BrightSens(2015), and European Project: ERC Proof of Concept,AmpliFISH

Subjects
0303 health sciences, fluorescent nanoparticles, mRNA, RNA imaging, General Engineering, General Physics and Astronomy, 010402 general chemistry, fluorescence microscopy, 01 natural sciences, 0104 chemical sciences, DNA-functionalized polymeric nanoparticles, 03 medical and health sciences, single-cell analysis, [CHIM]Chemical Sciences, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, General Materials Science, fluorescence in situ hybridization, 030304 developmental biology
Abstract

International audience; Detection and imaging of RNA at the single-cell level is of outmost importance for fundamental research and clinical diagnostics. Current techniques of RNA analysis, including fluorescence in situ hybridization (FISH), are long, complex and expensive. Here, we report a methodology of amplified FISH (AmpliFISH) that enables simpler and faster RNA imaging using small and ultrabright dyeloaded polymeric nanoparticles (NPs) functionalized with DNA. We found that the small size of NPs (below 20 nm) was essential for their access to the intracellular mRNA targets in fixed permeabilized cells. Moreover, proper selection of the polymer matrix of DNA-NPs minimized nonspecific intracellular interactions. Optimized DNA-NPs enabled sequence-specific imaging of different mRNA targets (survivin, actin and polyA tails), using a simple 1h staining protocol. Encapsulation of cyanine and rhodamine dyes with bulky counterions yielded green, red and far-red emitting NPs that were 2-100-fold brighter than corresponding quantum dots. These NPs enabled multiplexed detection of three mRNA targets simultaneously, showing distinctive mRNA expression profiles in three cancer cell lines. Image analysis confirmed the single-particle nature of the intracellular signal, suggesting single-molecule sensitivity of the method. AmpliFISH was found to be semi-quantitative, correlating with RT-qPCR. In comparison with the commercial LNA-based FISH technique, AmpliFISH provides 8-200-fold stronger signal (dependent on the NP color) and requires only 3 steps vs. ~20 steps together with much shorter time. Thus, combination of bright fluorescent polymeric NPs with FISH yields a fast and sensitive single-cell transcriptomic analysis method for RNA research and clinical diagnostics.

Published
2021

10. Alginate/Chitosan Compact Polyelectrolyte Complexes: A Cell and Bacterial Repellent Material

Author

Phoeung, Thida, primary, Spanedda, Maria Vittoria, additional, Roger, Emilie, additional, Heurtault, Béatrice, additional, Fournel, Sylvie, additional, Reisch, Andreas, additional, Mutschler, Angela, additional, Perrin-Schmitt, Fabienne, additional, Hemmerlé, Joseph, additional, Collin, Dominique, additional, Rawiso, Michel, additional, Boulmedais, Fouzia, additional, Schaaf, Pierre, additional, Lavalle, Philippe, additional, and Frisch, Benoît, additional

Published
2017
Full Text
View/download PDF

12. Neuronal Uptake and Neuroprotective Properties of Curcumin-Loaded Nanoparticles on SK-N-SH Cell Line: Role of Poly(lactide-co-glycolide) Polymeric Matrix Composition

Author

Djiokeng Paka, Ghislain, primary, Doggui, Sihem, additional, Zaghmi, Ahlem, additional, Safar, Ramia, additional, Dao, Lé, additional, Reisch, Andreas, additional, Klymchenko, Andrey, additional, Roullin, V. Gaëlle, additional, Joubert, Olivier, additional, and Ramassamy, Charles, additional

Published
2015
Full Text
View/download PDF

16. Cyto-mechanoresponsive Polyelectrolyte Multilayer Films

Author

Davila, Johanna, primary, Chassepot, Armelle, additional, Longo, Johan, additional, Boulmedais, Fouzia, additional, Reisch, Andreas, additional, Frisch, Benoît, additional, Meyer, Florent, additional, Voegel, Jean-Claude, additional, Mésini, Philippe J., additional, Senger, Bernard, additional, Metz-Boutigue, Marie-Hélène, additional, Hemmerlé, Joseph, additional, Lavalle, Philippe, additional, Schaaf, Pierre, additional, and Jierry, Loïc, additional

Published
2011
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results