1. Targeted Metabolite Analysis and Antioxidant Potential of Rumex induratus
- Author
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Patrícia Valentão, Luis M. Guerra, M. Angelo Rodrigues, Clara Pereira, Federico Ferreres, Rosa M. Seabra, Paula B. Andrade, and Paula Guedes de Pinho
- Subjects
Antioxidant ,Coumaric Acids ,Hypochlorous acid ,DPPH ,Metabolite ,medicine.medical_treatment ,Carboxylic Acids ,High-performance liquid chromatography ,Antioxidants ,chemistry.chemical_compound ,Phenols ,Antioxidant activity ,Rumex induratus ,Organic acids ,medicine ,Organic chemistry ,Rumex ,Chromatography, High Pressure Liquid ,Reactive nitrogen species ,Flavonoids ,chemistry.chemical_classification ,Chromatography ,General Chemistry ,Hydroxycinnamic acid ,Phenolic compounds ,Plant Leaves ,chemistry ,General Agricultural and Biological Sciences - Abstract
11 pages, 13 figures, 3 tables., Targeted metabolite analysis of aqueous extract of Rumex induratus leaves, in terms of phenolic compounds and organic acids, and the study of its antioxidant activity against the DPPH• radical, a reactive oxygen species, hypochlorous acid, and a reactive nitrogen species, nitric oxide (•NO), were performed. The samples were collected in several locations, spontaneously occurring or from greenhouse culture, at different stages of development and seasons. The phenolic composition was achieved by high-performance liquid chromatography (HPLC)−diode array detection, and four hydroxycinnamic acid derivatives and 10 flavonoid glycosides (C- and O-heterosides) were determined. Organic acids composition was established by HPLC-UV, revealing five compounds. The total amount of phenolic compounds and organic acids were affected by growing conditions and developmental phase. The aqueous extract exhibited a dose-related activity against all tested reactive species.
- Published
- 2008
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