1. Alkali Extraction of β-<scp>d</scp>-Glucans from Saccharomyces cerevisiae Cell Wall and Study of Their Adsorptive Properties toward Zearalenone
- Author
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Georges Jeminet, Jean Marie François, Claude-Gilles Dussap, Alexandros Yiannikouris, G. Bertin, Laurent Poughon, Jean-Pierre Jouany, Unité de Recherches sur les Herbivores (URH), Institut National de la Recherche Agronomique (INRA), Unité mixte de recherche biotechnologies bioprocédés, Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Centre Universitaire des Sciences et Techniques, Partenaires INRAE, Alltech-France, Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Synthèse et étude de systèmes à intêret biologique (SEESIB), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), and Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
- Subjects
cooperativity ,[SDV]Life Sciences [q-bio] ,Saccharomyces cerevisiae ,mycotoxin ,Cell wall ,03 medical and health sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Adsorption ,Cell Wall ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,[INFO]Computer Science [cs] ,[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering ,detoxification ,Solubility ,Glucans ,Zearalenone ,030304 developmental biology ,Glucan ,chemistry.chemical_classification ,0303 health sciences ,Chromatography ,biology ,Chemistry ,zearalenone ,Extraction (chemistry) ,yeast cell wall ,04 agricultural and veterinary sciences ,General Chemistry ,Hydrogen-Ion Concentration ,biology.organism_classification ,040401 food science ,Yeast ,alkali-soluble beta-D-glucans ,adsorption ,alkali-insoluble beta-D-glucans ,General Agricultural and Biological Sciences - Abstract
International audience; The isolated cell wall of Saccharomyces cerevisiae has some capacity to adsorb zearalenone (affinity near 30%) and reduce the bioavailability of toxins in the digestive tract. The adsorption process was quantified in vitro, and the data obtained when plotted with Hill's equation indicated a cooperative process. The model showed that the adsorption capacity was related to the yeast cell wall composition. This work focused on the role of various beta-D-glucan types in the efficacy of zearalenone adsorption by yeast cell wall and sought to elucidate some of the adsorption mechanisms. Zearalenone was mixed at 37 degreesC with a constant quantity of alkali-soluble or alkali-insoluble beta-D-glucans isolated from yeast cell walls, and the amount of adsorbed zearalenone was measured. Given that the alkali solubility of beta-D-glucans is a determining factor for their three-dimensional conformation and that the alkali-insoluble fraction had a greater affinity (up to 50%) than the alkali-soluble fraction (similar to16%), it was concluded that the three-dimensional structure strongly influences the adsorption process. The alkali insolubility of beta-D-glucans led to the formation of single and/or triple helices, which have been identified as the most favorable structures for zearalenone adsorption efficacy. The beta(1,3)-D-glucan and beta(1,6)-D-glucan compositions of the two alkali-extracted fractions and their involvement in the adsorption process are discussed.
- Published
- 2004
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