1. A Porphyrin Dimer–GdDOTA Conjugate as a Theranostic Agent for One- and Two-Photon Photodynamic Therapy and MRI
- Author
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Sébastien Jenni, Barbara Ventura, Célia S. Bonnet, Éva Tóth, Angélique Sour, Frédéric Bolze, Valérie Heitz, Agnès Pallier, Julie Schmitt, Institut de Chimie de Strasbourg, Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Conception et application de molécules bioactives (CAMB), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Istituto per la Sintesi Organica e la Fotoreattività (ISOF-CNR), Consiglio Nazionale delle Ricerche (CNR), Université de Strasbourg (UNISTRA), ISOF-CNR, Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and LEGOUPIL, Laëtitia
- Subjects
Porphyrins ,[SDV]Life Sciences [q-bio] ,medicine.medical_treatment ,MRI contrast agent ,Dimer ,Biomedical Engineering ,Pharmaceutical Science ,Bioengineering ,Photodynamic therapy ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Theranostic Nanomedicine ,HeLa ,chemistry.chemical_compound ,Nuclear magnetic resonance ,Two-photon excitation microscopy ,Heterocyclic Compounds ,Theranostic agent ,Neoplasms ,Organometallic Compounds ,medicine ,Humans ,[CHIM.COOR]Chemical Sciences/Coordination chemistry ,ComputingMilieux_MISCELLANEOUS ,Pharmacology ,Photons ,Photosensitizing Agents ,Singlet Oxygen ,biology ,Singlet oxygen ,Organic Chemistry ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Magnetic Resonance Imaging ,Porphyrin ,0104 chemical sciences ,3. Good health ,[SDV] Life Sciences [q-bio] ,Photochemotherapy ,chemistry ,Porphyrin dimer ,0210 nano-technology ,Dimerization ,MRI ,HeLa Cells ,Biotechnology ,Conjugate - Abstract
International audience; A molecular theranostic agent designed for photodynamic therapy (PDT) treatment in the near-infrared and for imaging tissue tumors with magnetic resonance imaging (MRI) is reported. It consists of a linear pi-conjugated Zn(II) porphyrin dimer linked at each extremity to a GdDOTA-type complex. This agent has shown very promising potential for PDT applications with good singlet oxygen generation in DMSO and high linear absorption in the near- infrared (lambda(max) = 746 nm, epsilon approximate to 10(5) M-1 cm(-1)). Moreover, this molecule has a propensity for two-photon excited PDT with high two-photon cross sections (similar to 8000 GM in 880-930 nm range), which should allow for deeper tumor treatments and higher spatial precision as compared to conventional one-photon PDT. Regarding the MRI contrast agent properties, the molecule has shown superior relaxivity (14.4 mM(-1) s(-1) at 40 MHz, 298 K) in comparison to clinical contrast agents and the ability to be internalized in cells, thanks to its amphiphilic character. Irradiation of HeLa cells using either one-photon (740 nm) or two-photon excitation (910 nm) has led in both cases to important cell death.
- Published
- 2018
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