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Conventional fabrication of microfluidic channels/devices faces challenges such as single use channels, material incompartibility, and/or significant time consumption. We propose a flexible platfor...

Characterization of signaling pathways in embryonic stem cells is a prerequisite for future application of these cells to treat human disease and other disorders. Identification of tyrosine signaling cascades is of particular interest but is complicated by the relatively low levels of tyrosine phosphorylation in embryonic stem cells. These hurdles correlate with the primary limitations of mass spectrometry-based proteomics; namely, poor detection limit and dynamic range. To overcome these obstacles, we fabricated miniaturized LC-electrospray assemblies that provided approximately 15-fold improvement in LC-MS performance. Significantly, our characterization data demonstrate that electrospray ionization efficiency compensates for diminished chromatographic performance at effluent flow rates below Van Deemter minima. Use of these assemblies facilitated quantitative proteomics-based analysis of tyrosine signaling cascades in embryonic stem cells. Our results suggest that a renewed focus on miniaturized LC coupled to ultralow flow electrospray will provide a viable path for proteomic analysis of primary cells and rare post-translational modifications.

A selected group of standard atactic polymers with CC, CO, diene, and SiO backbones and some crystalline polymers for which an amorphous phase T g is known have T g 's which increase with the product of cohesive energy density (CED) times the characteristic ratio (C ∞), falling on either of two straight lines of positive slope, intersecting at T g =118 K. The principal difference between polymers on the two lines concerns their low-frequency mechanical relaxation spectra: upper line polymers tend to have a relatively strong T β ∼ 0.75T g , but weak or missing T γ

In this experiment, students apply the technique of immobilized metal-ion affinity chromatography to the isolation of α-lactalbumin from milk whey.

Role d'espaceur inerte des motifs chlorure de vinylidene qui diminuent les associations intramoleculaires entre les dipoles carbonyl des motifs MMA du copolymere. Stabilite thermique minimale des copolymeres lorsque le nombre de motifs MMA et VDC adjacents est maximal; degradation thermique via une cyclisation intramoleculaire et perte de HCl pour donner une lactone a 5 chainons insatures

Isothermes de polymeres lineaires et ramifies entre 20 et 200°C a des pressions comprises entre 1 et 2000 bar a l'aide d'une forme lineaire de l'equation de Tait

Les enchevetrements physiques presents a forte masse moleculaire et haute concentration augmentent la stabilite du gel. Identification de trois regimes distincts sol-gel

A special project developed around the enzyme polyphenoloxidase or tyrosinase, studying its extraction, activity, inhibition, cofactor requirements, and kinetics.