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The streaming potential of mica covered by monodisperse latex particles was measured using the parallel-plate channel, four-electrode cell. The zeta potential of latex bearing amidine charged groups was regulated by the addition of NaCl (10(-4)-10(-2) M) and MgCl(2) (10(-4)-10(-2) M) at a constant pH 5.5 and by the change in pH (4-12) at 10(-2) M NaCl. The size of the latex particles, determined by dynamic light scattering, varied between 502 and 540 nm for the above electrolyte concentration range. Mica sheets have been covered with latex particles under diffusion transport conditions. The latex coverage was regulated by the bulk suspension concentration in the channel and the deposition time. The coverage was determined, with a relative precision of 2%, by the direct enumeration of particles by optical microscopy and AFM. The streaming potential of mica was then determined for a broad range of particle coverage 0 < theta < 0.5, the particle-to-substrate zeta potential ratio zeta(p)/zeta(i), and 8.8 < kappa a < 143 (thin double-layer limit). These experimental data confirmed that the streaming potential of covered surfaces is well reflected by the theoretical approach formulated in ref 32. It was also shown experimentally that variations in the substrate streaming potential with particle coverage for theta < 0.3 and zeta(p)/zeta(i) < 0 are characterized by a large slope, which enables the precise detection of particles attached to interfaces. However, measurements at high coverage and various pH values revealed that the apparent zeta potential of covered surfaces is 1/2(1/2) smaller than the bulk zeta potential of particles (in absolute terms). This is valid for arbitrary zeta potentials of substrates and particles, including the case of negative particles on negatively charged substrates that mimics rough surfaces. Therefore, it was concluded that the streaming potential method can serve as an efficient tool for determining bulk zeta potentials of colloids and bioparticles.