1. Biocatalytic Synthesis of Ruxolitinib Intermediate via Engineered Imine Reductase.
- Author
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Huang A, Zhang X, Yang Y, Shi C, Zhang B, Tuo X, Shen P, Jiao X, and Zhang N
- Subjects
- Imines chemistry, Imines metabolism, Molecular Structure, Hydrazines chemistry, Protein Engineering, Biocatalysis, Nitriles chemistry, Nitriles metabolism, Pyrimidines chemistry, Pyrimidines biosynthesis, Pyrimidines metabolism, Oxidoreductases metabolism, Oxidoreductases genetics, Pyrazoles chemistry, Pyrazoles metabolism
- Abstract
An enzyme catalyzed strategy for the synthesis of a chiral hydrazine from 3-cyclopentyl-3-oxopropanenitrile 5 and hydrazine hydrate 2 is presented. An imine reductase (IRED) from Streptosporangium roseum was identified to catalyze the reaction between 3-cyclopentyl-3-oxopropanenitrile 5 and hydrazine hydrate 2 to produce trace amounts of ( R )-3-cyclopentyl-3-hydrazineylpropanenitrile 4 . We employed a 2-fold approach to optimize the catalytic performance of this enzyme. First, a transition state analogue (TSA) model was constructed to illuminate the enzyme-substrate interactions. Subsequently, the Enzyme_design and Funclib methods were utilized to predict mutants for experimental evaluation. Through three rounds of site-directed mutagenesis, site saturation mutagenesis, and combinatorial mutagenesis, we obtained mutant M6 with a yield of 98% and an enantiomeric excess (ee) of 99%. This study presents an effective method for constructing a hydrazine derivative via IRED-catalyzed reductive amination of ketone and hydrazine. Furthermore, it provides a general approach for constructing suitable enzymes, starting from nonreactive enzymes and gradually enhancing their catalytic activity through active site modifications.
- Published
- 2024
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