1. Antibacterial and Hypoglycemic Diterpenoids from Salvia chamaedryoides
- Author
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Daniela Russo, Maria De Mieri, Matthias Hamburger, Angela Bisio, Nunziatina De Tommasi, Tiziano Tuccinardi, Silvana Alfei, Margherita Lapillo, Anna Maria Schito, Anita Parricchi, and Luigi Milella
- Subjects
ALPHA-AMYLASE INHIBITORS ,Molecular model ,ROSMARINUS-OFFICINALIS L ,CLERODANE DITERPENOIDS ,ANTIMICROBIAL ACTIVITY ,FERNALD LAMIACEAE ,GLUCOSIDASE ,CONSTITUENTS ,CORRUGATA ,HPLC ,Pharmaceutical Science ,01 natural sciences ,Analytical Chemistry ,Salvia chamaedryoides, hypoglycemic effects, antimicrobialactivity, Molecular modeling ,Molecular Medicine ,Pharmacology ,Drug Discovery ,Complementary and Alternative Medicine ,Dermatology ,Organic Chemistry ,Candida albicans ,Salvia ,Salvia chamaedryoides ,chemistry.chemical_classification ,Molecular Structure ,biology ,Antimicrobial ,Anti-Bacterial Agents ,Italy ,Diterpenes ,Two-dimensional nuclear magnetic resonance spectroscopy ,Staphylococcus aureus ,Stereochemistry ,Molecular modeling ,Microbial Sensitivity Tests ,Hypoglycemic Agents ,Glycoside Hydrolase Inhibitors ,Nuclear Magnetic Resonance, Biomolecular ,010405 organic chemistry ,Active site ,alpha-Glucosidases ,Plant Components, Aerial ,antimicrobialactivity ,biology.organism_classification ,hypoglycemic effects ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Enzyme ,Complementary and alternative medicine ,chemistry ,biology.protein ,Hypoglycemic Effects ,alpha-Amylases - Abstract
A surface extract of the aerial parts of Salvia chamaedryoides afforded 13 diterpenes (1-13), with seven compounds (1, 3, 4, 7-9, 12) described for the first time. The structures of the new compounds were established using 1D and 2D NMR spectroscopic methods, HRESIMS, and ECD data. The potential hypoglycemic effects of the crude extract, fractions, and pure compounds from S. chamaedryoides were investigated by inhibition of α-glucosidase and α-amylase enzymes. The extract and its fractions showed a moderate dose-dependent inhibition; the pure compounds exhibited differential inhibitory activity against these two enzymes. Molecular modeling studies were also performed to suggest the interaction mode of compound 3 in the α-glucosidase enzyme active site. The antimicrobial activity of the purified compounds was investigated against 26 clinical pathogens. No activity was detected for the Gram-negative species tested nor on Candida albicans and C. glabrata, while variable susceptibilities were observed using Gram-positive staphylococcal and enterococcal species.
- Published
- 2017