1. Automated protein hydrolysis delivering sample to a solid acid catalyst for amino acid analysis
- Author
-
Masuda, Akiko and Dohmae, Naoshi
- Subjects
Proteolysis -- Equipment and supplies ,Catalysts -- Usage ,Catalysts -- Composition ,Catalysts -- Materials ,Amino acids -- Chemical properties ,Chemistry - Abstract
In this study, we developed an automatic protein hydrolysis system using strong cation-exchange resins as solid acid catalysts. Examining several kinds of inorganic solid acids and cation-exchange resins, we found that a few cation-exchange resins worked as acid catalysts for protein hydrolysis when heated in the presence of water. The most efficient resin yielded amounts of amino acids that were over 70% of those recovered after conventional hydrolysis with hydrochloric acid and resulted in amino acid compositions matching the theoretical values. The solid-acid hydrolysis was automated by packing the resin into columns, combining the columns with a high-performance liquid chromatography system, and heating them. The amino acids that constitute a protein can thereby be determined, minimizing contamination from the environment. 10.1021/ac101718x
- Published
- 2010