1. Immobilization of cell wall invertase modified with glutaraldehyde for continuous production of invert sugar.
- Author
-
Vujcić Z, Milovanović A, Bozić N, Dojnov B, Vujcić M, Andjelković U, and Loncar N
- Subjects
- Cell Wall chemistry, Enzymes, Immobilized chemistry, Glutaral chemistry, Hydrogen-Ion Concentration, Saccharomyces cerevisiae chemistry, Saccharomyces cerevisiae enzymology, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins chemistry, Temperature, beta-Fructofuranosidase chemistry, Cell Wall enzymology, Enzymes, Immobilized metabolism, Fructose metabolism, Glucose metabolism, Saccharomyces cerevisiae Proteins metabolism, beta-Fructofuranosidase metabolism
- Abstract
Yeast cell wall invertase (CWI) was modified with dimethyl suberimidate, glutaraldehyde, formaldehyde, and sodium periodate. Retained activity after modification was 45% for CWI modified with formaldehyde, 77% for CWI modified with sodium periodate, 80% for CWI modified with glutaraldehyde, and 115% for CWI modified with dimethyl suberimidate. Chemically modified and native CWIs showed significantly broad pH stability (pH 3-11), whereas after incubations at 50, 60, and 70 °C, CWI modified with glutaraldehyde showed the highest thermostability. Optimum pH for CWI modified with glutaraldehyde was between 4 and 5, whereas optimum temperature was at 60 °C. Comparison to CWI modified with glutaraldehyde after immobilization within alginate beads showed broader pH optimum (4.0-5.5) as well as broader temperature optimum (55-70 °C). Column bed reactor packed with the immobilized CWI modified with glutaraldehyde was successfully used for the 95% inversion of 60% (w/w) sucrose at the flow rate of 3 bed volumes per hour, pH 4.9, and 45 °C. A 1 month productivity of 3844 kg of inverted sugar/kg of the immobilisate was obtained.
- Published
- 2010
- Full Text
- View/download PDF