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Your search keyword '"Lee ZW"' showing total 2 results
Start Over Author "Lee ZW" Publisher american chemical society
2 results on '"Lee ZW"'

1. Micropatterns of spores displaying heterologous proteins.

Author

Park TJ, Lee KB, Lee SJ, Park JP, Lee ZW, Choi SK, Jung HC, Pan JG, Lee SY, and Choi IS

Subjects
Bacillus thuringiensis chemistry, Bacillus thuringiensis genetics, Bacterial Proteins genetics, Biotechnology methods, Green Fluorescent Proteins, Luminescent Proteins biosynthesis, Luminescent Proteins genetics, Microscopy, Fluorescence, Nanotechnology methods, Recombinant Proteins biosynthesis, Recombinant Proteins genetics, Spores, Bacterial chemistry, Spores, Bacterial genetics, Bacillus thuringiensis metabolism, Bacterial Proteins biosynthesis, Spores, Bacterial metabolism
Abstract

Micropatterns of Bacillus thuringiensis spores were generated by a combination of surface chemistry, microcontact printing, and spore surface display technique. The outermost layers of B. thuringiensis spores were engineered to present enhanced green fluorescent protein (EGFP), and the biospecific interaction between biotin and streptavidin was utilized to spatially direct the EGFP-presenting spores onto the micropatterned surfaces. The viability of the micropatterned spores was confirmed by the pattern generation of vegetative cells after the germination.

Published
2004
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2. Pattern generation of biological ligands on a biodegradable poly(glycolic acid) film.

Author

Lee KB, Kim DJ, Lee ZW, Woo SI, and Choi IS

Subjects
Biodegradation, Environmental, Hydrolysis, Ligands, Membranes, Artificial, Microscopy, Atomic Force methods, Surface Properties, Biotin chemical synthesis, Oligopeptides chemical synthesis, Polyglycolic Acid chemistry
Abstract

The micropatterns of biological ligands (biotin and RGD peptides) were generated on a flat surface of biodegradable polymer, poly(glycolic acid) (PGA). The immobilization of biological ligands onto the surface of biodegradable polymers (especially aliphatic polyesters) is usually hampered by the absence of functionalizable groups on the polymer backbone. We demonstrate herein that PGA polymer films were modified by surface hydrolysis to introduce carboxylic acid groups on the film surfaces, which were subsequently used for patterning amine-terminated ligands by microcontact printing. Fluorescence microscopy was used to verify the pattern of biotin on the surface of the PGA films after complexation with fluorescein-conjugated streptavidin. In addition, the cellular micropatterns were obtained from micropatterns of RGD peptides on the surface-hydrolyzed PGA films.

Published
2004
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