1. Stability of Vegetal Diamine Oxidase in Simulated Intestinal Media: Protective Role of Cholic Acids.
- Author
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Neree AT, Pietrangeli P, Szabo PI, Mateescu MA, and Marcocci L
- Subjects
- Amine Oxidase (Copper-Containing) metabolism, Biogenic Amines metabolism, Cholic Acids chemistry, Enzyme Stability, Humans, Hydrogen-Ion Concentration, Intestines chemistry, Lathyrus chemistry, Lathyrus metabolism, Models, Biological, Plant Proteins genetics, Plant Proteins metabolism, Amine Oxidase (Copper-Containing) chemistry, Cholic Acids metabolism, Intestinal Mucosa metabolism, Lathyrus enzymology, Plant Proteins chemistry
- Abstract
Food biogenic amines, in particular, histamine, are often responsible for various enteric and vascular dysfunctions. Several years ago, the oral administration of copper-containing diamine oxidase (DAO), also called histaminase, able to oxidatively deaminate biogenic amines, had been suggested as a food supplement to control food allergy and enteric dysfunctions. This report is aimed to generate a global image on the behavior of orally administrated DAO dosage forms in the intestinal tract. The catalytic stability of DAO from Lathyrus sativus seedlings in various simulated intestinal media with different pH and containing different association of cholic acids, pancreatic proteases, bicarbonate, lipids, or alcohol was investigated. Cholic acids and lipids protected the enzyme in the simulated intestinal fluids. However, they were not able to protect against the inhibitory effect of 24-36% (v/v) ethanol. These observations may be relevant for oral administration of enzymes as food supplements or therapeutic bioactive agents.
- Published
- 2018
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