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Your search keyword '"Klomp, Leo W.J."' showing total 2 results
Start Over Author "Klomp, Leo W.J." Publisher american chemical society
2 results on '"Klomp, Leo W.J."'

1. Ratiometric fluorescent sensor proteins with subnanomolar affinity for Zn(II) based on copper chaperone domains

Author

van Dongen, Elisabeth M.W.M., Dekkers, Linda M., Spijker, Kristie, Meijer, E.W., Klomp, Leo W.J., and Merkx, Maarten

Subjects
Copper -- Chemical properties, Energy transformation -- Analysis, Homeostasis -- Analysis, Chemistry
Abstract

A study was conducted to develop a genetically encoded fluorescence resonance energy transfer-based sensor for copper(I) using copper-induced complex formation between these two protein domains. The effort provided insights into mechanism of transition metal homeostasis, particularly on metal selectivity and the role of protein-protein interactions.

Published
2006

2. Variation of linker length in ratiometric fluorescent sensor proteins allows rational tuning of Zn(II) affinity in the picomolar to femtomolar range

Author

van Dongen, Elisabeth M.W.M., Evers, Toon H., Dekkers, Linda M., Meijer, E.W., Klomp, Leo W.J., and Merkx, Maarten

Subjects
Peptide bonds -- Research, Peptide bonds -- Observations, Zinc -- Chemical properties, Zinc -- Spectra, Chemistry
Abstract

A demonstration that variation of peptide linker length and metal binding site engineering allows rational tuning of both the Zn(II) affinity and the ratiometric change in fusion proteins of cyan fluorescent protein fused to Atox1 (CFP-Atox1) and WD4-YFP is presented. Additionally, the importance of considering conformational distributions in the design of multidomain proteins is demonstrated.

Published
2007

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