16 results on '"Hsu, Ya-Wen"'
Search Results
2. Effects of Monascin on Anti-inflammation Mediated by Nrf2 Activation in Advanced Glycation End Product-Treated THP-1 Monocytes and Methylglyoxal-Treated Wistar Rats.
- Author
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Lee, Bao-Hong, Hsu, Wei-Hsuan, Tao Huang, Chang, Yu-Ying, Hsu, Ya-Wen, and Pan, Tzu-Ming
- Published
- 2013
- Full Text
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3. Monascin and Ankaflavin Have More Anti-atherosclerosis Effect and Less Side Effect Involving Increasing Creatinine Phosphokinase Activity than Monacolin K under the Same Dosages.
- Author
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Lee, Chun-Lin, Hung, Yu-Ping, Hsu, Ya-Wen, and Pan, Tzu-Ming
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- 2013
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4. Dimerumic Acid and Deferricoprogen Activate Ak Mouse Strain Thymoma/Heme Oxygenase-1 Pathways and Prevent Apoptotic Cell Death in 6-Hydroxydopamine-Induced SH-SY5Y Cells.
- Author
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Tseng WT, Hsu YW, and Pan TM
- Subjects
- Antioxidants therapeutic use, Cell Line, Tumor, Cell Survival, Humans, Oryza chemistry, Oxidative Stress, Oxidopamine chemistry, Parkinson Disease metabolism, Reactive Oxygen Species metabolism, Apoptosis drug effects, Diketopiperazines chemistry, Heme Oxygenase-1 metabolism, Hydroxamic Acids chemistry, Proto-Oncogene Proteins c-akt metabolism
- Abstract
Parkinson's disease (PD) is a neurodegenerative disorder, which can be modeled using the neurotoxin 6-hydroxydopamine (6-OHDA) to generate oxidative stress. Here, we studied the effects of the antioxidants deferricoprogen (DFC) and dimerumic acid (DMA), produced by rice fermented with Monascus purpureus NTU 568, on 6-OHDA-induced apoptosis in SH-SY5Y cells and their potential protective mechanisms. DMA and DFC inhibited 6-OHDA-induced apoptosis and cellular reactive oxygen species (ROS) in SH-SY5Y human neuroblastoma cells. Molecular analysis demonstrated associated upregulation of the Ak mouse strain thymoma (Akt), heme oxygenase-1 (HO-1), and signal-regulated kinase (ERK) pathways along with inhibited phosphorylation of c-Jun N-terminal kinase (JNK) and p38 pathways and altered homodimeric glycoprotein, N-methyl-d-aspartate (NMDA) receptor, and immunoglobulin Fc receptor gene expression. These results suggested that the neuroprotection elicited by DMA and DFC against 6-OHDA-induced neurotoxicity was associated with the Akt, MAPK, and HO-1 pathways via regulating the gene expression of NMDA receptor, homodimeric glycoprotein, and immunoglobulin Fc receptor.
- Published
- 2016
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5. Inhibition of Th2 cytokine production in T cells by monascin via PPAR-γ activation.
- Author
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Hsu WH, Lee BH, Hsu YW, and Pan TM
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- Animals, Cell Line, Cytokines genetics, Mice, Monascus metabolism, Oryza metabolism, Oryza microbiology, PPAR gamma genetics, Phosphorylation drug effects, Signal Transduction drug effects, T-Lymphocytes immunology, Th2 Cells immunology, Cytokines immunology, Heterocyclic Compounds, 3-Ring pharmacology, Monascus chemistry, PPAR gamma immunology, T-Lymphocytes drug effects, Th2 Cells drug effects
- Abstract
Yellow pigment monascin (MS) is a secondary metabolite isolated from Monascus -fermented products and has numerous physiological activities. However, the potential use of MS for immunomodulation remains unclear. We showed that MS and the synthetic peroxisome proliferator-activated receptor (PPAR)-γ ligand rosiglitazone (RG) significantly inhibited the production of Th2 cytokines, including IL-4, IL-5, and IL-13, in PMA/ionomycin-activated mouse EL-4 T cells. Moreover, we showed that this was due to cellular PPAR-γ translocation. These results indicate that MS and RG promote PPAR-γ-DNA interactions and suggest that the regulatory effects of MS and RG on Th2 cytokine production could be abolished with PPAR-γ antagonist treatment. MS and RG also suppressed Th2 transcription factor translocation (e.g., GATA-3 and nuclear factor of activated T cells) by preventing the phosphorylation of protein kinase C and signal transducer and activator of transcription 6.
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- 2013
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6. Peroxisome proliferator-activated receptor-γ activators monascin and rosiglitazone attenuate carboxymethyllysine-induced fibrosis in hepatic stellate cells through regulating the oxidative stress pathway but independent of the receptor for advanced glycation end products signaling.
- Author
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Hsu WH, Lee BH, Hsu YW, and Pan TM
- Subjects
- Animals, Anti-Inflammatory Agents pharmacology, Cells, Cultured, Hepatic Stellate Cells drug effects, Hepatic Stellate Cells pathology, Liver Cirrhosis prevention & control, Lysine pharmacology, Male, Mice, PPAR gamma drug effects, RNA, Small Interfering genetics, Receptor for Advanced Glycation End Products, Receptors, Immunologic antagonists & inhibitors, Receptors, Immunologic physiology, Rosiglitazone, Signal Transduction drug effects, Transfection, beta-Glucans, Heterocyclic Compounds, 3-Ring pharmacology, Liver Cirrhosis chemically induced, Lysine analogs & derivatives, Oxidative Stress drug effects, PPAR gamma physiology, Thiazolidinediones pharmacology
- Abstract
Advanced glycation end products (AGEs) signaling through its receptors (RAGE) results in an increase in reactive oxygen species (ROS) and is thought to contribute to hepatic fibrosis via hyperglycemia. Carboxymethyllysine (CML) is a key AGE, with highly reactive dicarbonyl metabolites. We investigated the inhibitory effect of Monascus -fermented metabolite monascin and rosiglitazone on CML-induced RAGE signaling in hepatic stellate cells (HSCs) and its resulting antihepatic fibrosis activity. We found that monascin and rosiglitazone upregulated peroxisome proliferator-activated receptor-γ (PPAR-γ) to attenuate α-smooth muscle actin (SMA) and ROS generation in CML-treated HSCs in a RAGE activation-independent pathway. Therefore, monascin may delay or inhibit the progression of liver fibrosis through the activation of PPAR-γ and might prove to be a major antifibrotic mechanism to prevent liver disease.
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- 2013
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7. Monascin and AITC attenuate methylglyoxal-induced PPARγ phosphorylation and degradation through inhibition of the oxidative stress/PKC pathway depending on Nrf2 activation.
- Author
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Hsu WH, Lee BH, Li CH, Hsu YW, and Pan TM
- Subjects
- Animals, Mice, Mice, Inbred BALB C, NF-E2-Related Factor 2 metabolism, PPAR gamma genetics, Phosphorylation drug effects, Protein Kinase C genetics, Proteolysis drug effects, Heterocyclic Compounds, 3-Ring pharmacology, Isothiocyanates pharmacology, NF-E2-Related Factor 2 genetics, Oxidative Stress drug effects, PPAR gamma metabolism, Protein Kinase C metabolism, Pyruvaldehyde pharmacology
- Abstract
Abnormal cellular accumulation of the dicarbonyl metabolite methylglyoxal (MG) results in cell damage, inflammation, and oxidative stress. It is also associated with increased protein linkage to form advanced glycation end products (AGEs) or induce DNA strand breaks. The association between peroxisome proliferator-activated receptor-γ (PPARγ) and nuclear factor-erythroid 2-related factor 2 (Nrf2) is unclear. This study investigated Nrf2 activator protection against PPARγ phosphorylation and degradation to maintain pancreatic function. MG was used at a noncytotoxic concentration (200 μM) to induce protein kinase C (PKC) and PPARγ phosphorylation in pancreatic RINm5F cells. For in vivo studies, MG (60 mg/kg bw) was intraperitoneally (IP) injected into Balb/C mice for 28 d to induce pancreas damage, at which point we investigated the effect of monascin protection (PPARγ and Nrf2 activator), rosiglitazone (PPARγ activator), allyl isothiocyanate (AITC; Nrf2 activator), or N-acetylcysteine (NAC) on pancreatic function. The in vitro and in vivo results indicated that MG leads to marked PPARγ phosphorylation (serine 82); this effect led to reduction in pancreatic and duodenal homeobox-1 (PDX-1), glucokinase (GCK), and insulin expression. However, monascin and rosiglitazone may protect PPARγ degradation by elevating PDX-1, GCK, and as a result, insulin expression. Monascin and AITC can attenuate PKC activation to suppress PPARγ phosphorylation caused by oxidative stress through the Nrf2 pathway. Similarly, the N-acetylcysteine (NAC) antioxidant also improved oxidative stress and pancreatic function. This study examined whether MG caused impairment of PDX-1, GCK, and insulin through PPARγ phosphorylation and degradation. MG and AGE accumulation improved on Nrf2 activation, thereby protecting against pancreas damage. Taken together, PPARγ activation maintained pancreatic PDX-1, GCK, and insulin expression levels to regulate blood glucose levels.
- Published
- 2013
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8. Anti-inflammatory properties of yellow and orange pigments from Monascus purpureus NTU 568.
- Author
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Hsu LC, Liang YH, Hsu YW, Kuo YH, and Pan TM
- Subjects
- Animals, Anti-Inflammatory Agents metabolism, Color, Edema genetics, Edema immunology, Humans, Interleukin-1beta genetics, Interleukin-1beta immunology, Macrophages drug effects, Macrophages immunology, Male, Mice, Mice, Inbred BALB C, Monascus metabolism, Nitric Oxide Synthase Type II genetics, Nitric Oxide Synthase Type II immunology, Pigments, Biological metabolism, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha immunology, Anti-Inflammatory Agents pharmacology, Edema drug therapy, Monascus chemistry, Pigments, Biological administration & dosage
- Abstract
The Monascus species has been used in foods for thousands of years in China. In this study, 10 azaphilone pigments, including four yellow and six orange pigments, were isolated from the fermented rice and dioscorea of Monascus purpureus NTU 568. By employing lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells, we determined the inhibitory activities of these pigments on nitric oxide (NO) production. As a result, four orange pigments, monaphilols A-D, showed the highest activities (IC50 = 1.0-3.8 μM), compared with the other two orange pigments, monascorubrin (IC50 > 40 μM) and rubropunctatin (IC50 = 21.2 μM), and the four yellow pigments ankaflavin (IC50 = 21.8 μM), monascin (IC50 = 29.1 μM), monaphilone A (IC50 = 19.3 μM), and monaphilone B (IC50 = 22.6 μM). Using Western blot and ELISA kits, we found that treatments with 30 μM of the yellow pigments and 5 μM of the orange pigments could down-regulate the protein expression of inducible nitric oxide synthase (iNOS) and suppress the production of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). We also used two animal experiments to evaluate the anti-inflammatory effects of these pigments. In a 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema model, eight of these pigments (0.5 mg/ear) could prevent ear edema against TPA administrations on the ears of BALB/c mice. In an LPS-injection mice model, several of these pigments (10 mg/kg) could inhibit the NO, TNF-α, IL-1β, and IL-6 levels in the plasma of BALB/c mice. As concluded from the in vitro and in vivo studies, six azaphilonoid pigments, namely, ankaflavin, monaphilone A, and monaphilols A-D, showed high potential to be developed into chemopreventive foods or drugs against inflammation-associated diseases.
- Published
- 2013
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9. Monascus-fermented yellow pigments monascin and ankaflavin showed antiobesity effect via the suppression of differentiation and lipogenesis in obese rats fed a high-fat diet.
- Author
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Lee CL, Wen JY, Hsu YW, and Pan TM
- Subjects
- Adipocytes drug effects, Adipocytes metabolism, Animals, CCAAT-Enhancer-Binding Protein-alpha genetics, CCAAT-Enhancer-Binding Protein-alpha metabolism, CCAAT-Enhancer-Binding Protein-beta genetics, CCAAT-Enhancer-Binding Protein-beta metabolism, Creatine Kinase metabolism, Diet, High-Fat, Dose-Response Relationship, Drug, Fermentation, Lipolysis drug effects, Lovastatin pharmacology, Male, Membrane Transport Proteins genetics, Membrane Transport Proteins metabolism, Obesity drug therapy, PPAR gamma genetics, PPAR gamma metabolism, Rats, Rats, Sprague-Dawley, Anti-Obesity Agents pharmacology, Cell Differentiation drug effects, Flavins pharmacology, Heterocyclic Compounds, 3-Ring pharmacology, Lipogenesis drug effects, Monascus chemistry
- Abstract
Monascus-fermented monascin and ankaflavin are found to strongly inhibit differentiation and lipogenesis and stimulate lipolysis effects in a 3T3-L1 preadipocyte model, but the in vivo regulation mechanism is unclear. This study uses obese rats caused by a high-fat diet to examine the effects of daily monascin and ankaflavin feeding (8 weeks) on antiobesity effects and modulation of differentiation, lipogenesis, and lipid absorption. The results show that monascin and ankaflavin had a significant antiobesity effect, which should result from the modulation of monascin and ankaflavin on the inhibition of differentiation by inhibiting CCAT/enhancer-binding protein β (C/EBPβ) expression (36.4% and 48.3%) and its downstream peroxisome proliferator-activated receptor γ (PPARγ) (55.6% and 64.5%) and CCAT/enhancer-binding protein α (C/EBPα) expressions (25.2% and 33.2%) and the inhibition of lipogenesis by increasing lipase activity (14.0% and 10.7%) and decreasing heparin releasable lipoprotein lipase (HR-LPL) activity (34.8% and 30.5%). Furthermore, monascin and ankaflavin are the first agents found to suppress Niemann-Pick C1 Like 1 (NPC1L1) protein expression (73.6% and 26.1%) associated with small intestine tissue lipid absorption. Importantly, monascin and ankaflavin are not like monacolin K, which increases creatine phosphokinase (CPK) activity, known as a rhabdomyolysis indicator.
- Published
- 2013
- Full Text
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10. Protective effect of deferricoprogen isolated from Monascus purpureus NTU 568 on citrinin-induced apoptosis in HEK-293 cells.
- Author
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Hsu LC, Hsu YW, Liang YH, Lin ZH, Kuo YH, and Pan TM
- Subjects
- Antioxidants isolation & purification, Caspases metabolism, Citrinin toxicity, Diketopiperazines isolation & purification, Fermentation, HEK293 Cells, Humans, Hydroxamic Acids isolation & purification, Kidney Diseases chemically induced, Kidney Diseases prevention & control, Oryza chemistry, Oryza microbiology, Antioxidants pharmacology, Apoptosis drug effects, Citrinin pharmacology, Diketopiperazines pharmacology, Hydroxamic Acids pharmacology, Monascus chemistry
- Abstract
Monascus species have traditionally been used in Asian food, with rice as their fermentation substrate. Red mold rice (RMR) contains citrinin, a nephrotoxic agent capable of exerting oxidative stress and cellular apoptosis. We investigated the components in RMR that could minimize the adverse effects of citrinin. Combining chemical separations and bioactivity assays, we identified an antioxidative component called deferricoprogen (DFC) in the fermented rice of Monascus purpureus NTU 568. The DFC structure was confirmed by nuclear magnetic resonance (NMR) and mass spectra analysis. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical-scavenging activity of DFC was similar to that of vitamin E. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay and flow cytometric analysis showed the effect of DFC and citrinin on cell viability and cell cycle. DFC was found to be protective against the cytotoxicity and cell death induced by citrinin on human embryonic kidney (HEK-293) cells. DFC also demonstrated anti-apoptotic property in preventing citrinin-induced apoptosis.
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- 2012
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11. New bioactive orange pigments with yellow fluorescence from Monascus-fermented dioscorea.
- Author
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Hsu YW, Hsu LC, Liang YH, Kuo YH, and Pan TM
- Subjects
- Animals, Cell Line, Tumor, Cell Proliferation drug effects, Dioscorea chemistry, Dioscorea metabolism, Fluorescence, Humans, Molecular Structure, Pigments, Biological chemistry, Dioscorea microbiology, Fermentation, Monascus metabolism, Pigments, Biological metabolism, Pigments, Biological pharmacology
- Abstract
Red mold dioscorea (RMD) is a fermented product of Monascus purpureus NTU 568 using dioscorea as culture substrate. To investigate the bioactive components of RMD, six orange pigments including four new azaphilones with yellow fluorescence, monapilol A-D (1-4), and known monascorubrin (5) and rubropunctatin (6) were isolated and characterized. Structural elucidation of new isolates was based on nuclear magnetic resonance ((1)H NMR, (13)C NMR, COSY, HMQC, and HMBC) and other spectroscopic analyses. The structures of monapilols (1-4) were similar to those of monascorubrin (5) and rubropunctatin (6); however, the hydroxyl group (8-OH) in compounds 1-4 substituted for the C-8 carbonyl in compounds 5 and 6. Biological evaluation indicated that compounds 1-4 inhibited nitric oxide (NO) production on lipopolysaccharide-stimulated RAW 264.7 cells. Compounds 1-4 also exhibited antiproliferative activities against human laryngeal carcinoma (HEp-2) and human colon adenocarinoma (WiDr).
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- 2011
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12. Anti-tumor and anti-inflammatory properties of ankaflavin and monaphilone A from monascus purpureus NTU 568.
- Author
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Hsu LC, Hsu YW, Liang YH, Kuo YH, and Pan TM
- Subjects
- Animals, Apoptosis, Caspases metabolism, Cell Line, Cell Line, Tumor, Cyclooxygenase 2 analysis, Humans, Laryngeal Neoplasms, Macrophages, Mice, Nitric Oxide Synthase Type II analysis, Anti-Inflammatory Agents pharmacology, Antineoplastic Agents pharmacology, Benzopyrans pharmacology, Flavins pharmacology, Monascus chemistry
- Abstract
An azaphilonidal derivative monaphilone A (MA) was recently isolated from the fermented products of Monascus purpureus NTU 568 by our laboratory. We report here the exploration of apoptosis-related and anti-inflammatory properties of MA and ankaflavin (AK) by some experiments about inducing death of human laryngeal carcinoma cell line HEp-2 and reducing inflammatory responses on murine macrophage RAW 264.7 cells. We employed a ssDNA enzyme-linked immunosorbent assay (ELISA) kit to investigate the nuclear changes of early apoptosis induced by AK and MA on HEp-2 cells and used a western blot and an enzyme activity assay to demonstrate the activation of caspase-3, caspase-8, and caspase-9 by MA and AK. Our studies revealed that AK and MA may decrease lipopolysaccharide (LPS)-induced inflammatory responses, including nitrite productions and expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in RAW 264.7 cells. All evidence support that azaphilonidal derivatives from M. purpureus NTU 568, such as AK and MA, are suitable for the development of chemotherapy or chemopreventive agents.
- Published
- 2011
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13. The effect of Monascus secondary polyketide metabolites, monascin and ankaflavin, on adipogenesis and lipolysis activity in 3T3-L1.
- Author
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Jou PC, Ho BY, Hsu YW, and Pan TM
- Subjects
- 3T3-L1 Cells, Adipocytes cytology, Adipocytes enzymology, Adipocytes metabolism, Animals, Flavins metabolism, Heterocyclic Compounds, 3-Ring metabolism, Lipoprotein Lipase metabolism, Mice, Monascus chemistry, Adipocytes drug effects, Adipogenesis drug effects, Flavins pharmacology, Heterocyclic Compounds, 3-Ring pharmacology, Lipolysis drug effects, Macrolides metabolism, Monascus metabolism
- Abstract
The aim of the present work is to investigate the effects of Monascus secondary metabolites, monascin (MS) and ankaflavin (AK), on cell proliferation, adipogenesis, lipolysis and heparin-releasable lipoprotein lipase (HR-LPL) in 3T3-L1 preadipocyte. MS and AK inhibit the proliferation of 3T3-L1 cells in a dose-dependent fashion. At 8 μg/mL concentration MS inhibits proliferation for 80.5% after 48 h, whereas the value for AK is 69.2%. Adipogenesis is inhibited by MS and AK without dose-dependency. Triglyceride is decreased 37.1% and 41.1% respectively by treating 0.125 μg/mL MS and AK. Adipocyte-specific transcription factors peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein β (C/EBPβ), C/EBPδ and C/EBPα mRNA levels are measured by real-time polymerase chain reaction. The expression of the four transcriptional factors analyzed (PPARγ, C/EBPβ, C/EBPδ and C/EBPα) is reduced at the initial and the middle period. At the later period, there is no effect on the expression of PPARγ and C/EBPα by treating MS and AK. Furthermore, both MS and AK increase basal lipolysis of mature adipocytes by 113.2% and 278.3% upregulation, respectively. And both MS and AK reduce the activity of HR-LPL, by 45.3% and 58.1% reduction, respectively. This study reveals for the first time that Monascus secondary metabolites, MS and AK, can prevent the differentiation of preadipocyte and stimulate basal lipolysis of mature adipocytes, avoiding the accumulation of lipid.
- Published
- 2010
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14. Monascin and ankaflavin act as novel hypolipidemic and high-density lipoprotein cholesterol-raising agents in red mold dioscorea.
- Author
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Lee CL, Kung YH, Wu CL, Hsu YW, and Pan TM
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- Animals, Basal Metabolism drug effects, Bone Density drug effects, Cricetinae, Lipid Peroxidation drug effects, Male, Mesocricetus, Cholesterol, HDL blood, Dioscorea metabolism, Flavins pharmacology, Heterocyclic Compounds, 3-Ring pharmacology, Hypolipidemic Agents pharmacology
- Abstract
Monascus-fermented red mold dioscorea (RMD) has been proven to possess greater hypolipidemic effect than red mold rice (RMR) even though they include equal levels of cholesterol-lowering agent monacolin K. However, higher concentrations of yellow pigments (monascin and ankaflavin) were found in RMD than in RMR. In this study, purified monascin and ankaflavin were administered to hyperlipidemic hamsters for 8 weeks, respectively, to test whether these two compounds were novel hypolipidemic ingredients. In the statistical results, monascin and ankaflavin showed significant effect on lowering cholesterol, triglyceride, and low-density lipoprotein cholesterol levels in serum, as well as aorta lipid plaque (p < 0.05). Importantly, monascin and ankaflavin, unlike monacolin K, were able to perform up-regulation rather than down-regulation on high-density lipoprotein cholesterol (HDL-C) levels in serum. This finding not only explained why RMD showed greater hypolipidemic and HDL-C-raising effect than RMR but also proved that monascin and ankaflavin would act as novel and potent hypolipidemic ingredients.
- Published
- 2010
- Full Text
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15. Monaphilones A-C, three new antiproliferative azaphilone derivatives from Monascus purpureus NTU 568.
- Author
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Hsu YW, Hsu LC, Liang YH, Kuo YH, and Pan TM
- Subjects
- Benzopyrans isolation & purification, Cell Line, Tumor, Cell Proliferation drug effects, Cyclohexanones isolation & purification, Growth Inhibitors isolation & purification, Humans, Pigments, Biological isolation & purification, Benzopyrans pharmacology, Cyclohexanones pharmacology, Growth Inhibitors pharmacology, Monascus chemistry, Pigments, Biological pharmacology
- Abstract
Monascus purpureus NTU 568 was a mutant strain from M. purpureus HM105. The methanol extract of red mold rice fermented by this strain exhibited four major yellow pigment signals on HPLC profile. By repeated chemical chromatography methods, three new azaphilone derivatives, namely, monaphilone A (1), B (2) and C (3), along with the known pigments ankaflavin (4) and monascin (5), were isolated and characterized. Based on spectroscopic analyses, mainly 1D and 2D NMR data, the structures of compounds 1-3 were completely elucidated; in addition, 1-3 were determined to be new azaphilone structures, due to the decrease of carbon monoxide for producing a gamma-lactone ring, compared with other azaphilone derivatives. Biological evaluations showed that monaphilone A (1) and B (2) exhibited an antiproliferative effect against HEp-2 (human laryngeal carcinoma cell line) and WiDr (human colon adenocarcinoma cell line), and none of the five compounds had toxicity to normal human lung cell lines (WI-38 and MRC-5) at 70 muM.
- Published
- 2010
- Full Text
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16. New dammarane-type saponins from the galls of Sapindus mukorossi.
- Author
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Kuo YH, Huang HC, Yang Kuo LM, Hsu YW, Lee KH, Chang FR, and Wu YC
- Subjects
- Cell Death drug effects, Humans, Saponins chemistry, Saponins pharmacology, Tumor Cells, Cultured, Dammaranes, Plant Tumors, Sapindus chemistry, Saponins analysis, Triterpenes analysis
- Abstract
Five new dammarane-type saponins, 3beta,7beta,20(S),22-tetrahydroxydammar-24-ene-3-O-alpha-l-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside, 3beta,7beta,20(S),22,23-pentahydroxydammar-24-ene-3-O-alpha-l-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside, 3beta,7beta,20(S),22,25-pentahydroxydammar-23-ene-3-O-alpha-l-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside, 25-methoxy-3beta,7beta,20(S),22-tetrahydroxydammar-23-ene-3-O-alpha-l-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside, and 25-methoxy-3beta,7beta,20(R)-trihydroxydammar-23-ene-3-O-alpha-l-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside, named sapinmusaponins A (1), B (2), C (3), D (4), and E (5), respectively, together with three known phenylpropanoid glycosides (6-8), were isolated from the galls of Sapindus mukorossi. The structures of these saponins were elucidated on the basis of spectroscopic analyses and chemical methods. Preliminary bioassay data revealed that saponins 1 and 3-5 showed moderate cytotoxic activity (ED50 approximately 9-18 microg/mL) against human tumor cell lines (Hepa59T/VGH, NCI, HeLa, and Med) and that 1-5 were inactive in vitro against HIV replication in H9 lymphocytes.
- Published
- 2005
- Full Text
- View/download PDF
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