1. Coordination Tendency of N-Acetylamino Acids, Nucleotides, and DNA Toward the Luminescent Bioprobes Tb(III)-Bathophenanthroline or Tb(III)-Anthracene-9-Carboxylic Acid.
- Author
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Hassan A. Azab, S. S. Al-Deyab, Zeinab M. Anwar, and Rasha G. Ahmed
- Subjects
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AMINO acids , *NUCLEOTIDES , *DNA , *LUMINESCENT probes , *PHENANTHROLINE , *ANTHRACENE , *CARBOXYLIC acids , *INFRARED spectra , *THERMAL analysis - Abstract
The solid complex Tb(III)-bathophenanthroline (Bphen) was synthesized and characterized by elemental analysis, IR spectra, and thermalanalysis. The formation of binary and ternary complexes of Tb(III) with N-acetylamino acids (N-acetylaspartic acid, N-acetylhistidine, and N-acetylhistamine), and Bphen or anthracene-9-carboxylic acid (9-ANCA) has been studied potentiometrically at (25.0 ± 0.1) °C and an ionic strength of I= 0.1 mol·dmâ3. The results obtained confirmed the recognition of the investigated N-acetylamino acids by the luminescent probes used at the molecular level. The formation of binary and ternary complexes of Tb(III) with nucleotides guanosine 5â²-monophosphate (5â²-GMP), adenosine 5â²-monophosphate (5â²-AMP), cytidine 5â²-monophosphate (5â²-CMP), or N-acetylamino acids (N-acetylaspartic acid, N-acetylhistidine, and N-acetylhistamine) has been studied potentiometrically. Confirmation of the formation of the ternary systems of the type Tb(III)-Bphen-N-acetylamino acids or Tb(III)-9-ANCA-N-acetylamino acid in solution has been carried out using UV absorption spectra. The interaction of Tb(III)-Bphen with calf-thymus DNA (CT-DNA) was monitored using electrochemical measurements at a glassy carbon electrode via cyclic voltammetry and square wave voltammetry (SWV) in phosphate buffer (pH 7.00). The data reveal the preferential interaction of Tb(III)-Bphen with the guanine and adenine residues of CT-DNA. Fluorescence measurements have been carried out to investigate the interaction of Tb(III)-Bphen with CT-DNA and nucleotides 5â²-GMP, 5â²-AMP, and 5â²-CMP. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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