1. Proteomic analysis of mature melanosomes from the retinal pigmented epithelium.
- Author
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Azarian SM, McLeod I, Lillo C, Gibbs D, Yates JR, and Williams DS
- Subjects
- Animals, Cells, Cultured, Epithelial Cells chemistry, Epithelial Cells cytology, Epithelial Cells ultrastructure, Mass Spectrometry, Melanosomes ultrastructure, Microscopy, Immunoelectron, Pigment Epithelium of Eye cytology, Pigment Epithelium of Eye ultrastructure, Proteome metabolism, Retina cytology, Retina ultrastructure, Swine, Melanosomes chemistry, Melanosomes metabolism, Pigment Epithelium of Eye chemistry, Proteome chemistry, Retina chemistry
- Abstract
The protein content of melanosomes in the retinal pigment epithelium (RPE) was analyzed by mass spectrometry. More than 100 proteins were found to be common to two out of three variations of sample preparation. Some proteins normally associated with other organelles were detected. Several lysosomal enzymes were detected, with the presence of cathepsin D confirmed by immunoelectron microscopy, thus supporting the previously suggested notion that melanosomes may contribute to the degradation of ingested photoreceptor outer segment disks.
- Published
- 2006
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