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Your search keyword '"Wu, Jianhua"' showing total 2 results
Start Over Author "Wu, Jianhua" Topic reactivity (chemistry) Publisher american chemical society
2 results on '"Wu, Jianhua"'

1. Helix Packing In The Lactose Permease of Escherichia Coli: Distances Between Site-Directed...

Author

Voss, John, Wu, Jianhua, Hubbell, Wayne L., Jacques, Vincent, Meares, Claude F., and Kaback, H. Ronald

Subjects
*LACTOSE, *NITROXIDES, *REACTIVITY (Chemistry)
Abstract

Investigates the helix packing in the lactose permease of Escherichia coli. Distances between site-directed nitroxides and a lanthanide; Substrate protection of Cys148 in lactose permease; Specific direction of a methanethiosulfonate nitroxide spin-label to one of two Cys residues in a double-Cys mutant; Nitroxide-nitroxide interactions.

Published
2001
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2. Cysteine 148 in the lactose permease of Escherichia coli is a component of a substrate binding site.

Author

Wu, Jianhua and Kaback, H. Ronald

Subjects
*ENZYMES, *AMINO acids, *BACTERIAL genetics, *ESCHERICHIA coli, *FLUORESCENCE spectroscopy, *REACTIVITY (Chemistry), *STRUCTURE-activity relationships
Abstract

Describes the use of site-directed fluorescence labeling to provide more direct evidence for the contention that cysteine (Cys) 148 is located in a binding site in the lactose (lac) permease of Escherichia coli. Plasmid construction; Purification of biotinated lac permease with single Cys residues; Protein determinations; Fluorescence quenching studies.

Published
1994
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