1. Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.
- Author
-
Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwartz JF, and Shaper JH
- Subjects
- Acute Disease, Animals, Antibodies, Monoclonal biosynthesis, Antigen-Antibody Reactions, Antigens, Surface isolation & purification, Bone Marrow immunology, Bone Marrow Cells, Cell Line, Cell Separation, Chemical Precipitation, Female, Flow Cytometry, Hematopoietic Stem Cells cytology, Hematopoietic Stem Cells pathology, Humans, Leukemia immunology, Leukemia pathology, Mice, Mice, Inbred BALB C, Antibodies, Monoclonal immunology, Antigens, Surface immunology, Hematopoietic Stem Cells immunology, Leukemia, Erythroblastic, Acute immunology
- Abstract
The anti-My-10 mouse monoclonal antibody was raised against the immature human myeloid cell line KG-1a and was selected for nonreactivity with mature human granulocytes. Anti-My-10 immunoprecipitated a KG-1a cell surface protein with an apparent Mr of approximately 115 kD. We describe the binding of this antibody to human hematopoietic cell types and show that My-10 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells. My-10 is also expressed by leukemic marrow cells from a subpopulation of patients. Thus, this antibody allows the identification and purification of hematopoietic progenitor cells from normal human marrow and the subclassification of leukemia.
- Published
- 1984