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Start Over Author "M., Fagiolini" Publisher american association for the advancement of science
8 results on '"M., Fagiolini"'

1. Transparent arrays of bilayer-nanomesh microelectrodes for simultaneous electrophysiology and two-photon imaging in the brain.

Author

Qiang Y, Artoni P, Seo KJ, Culaclii S, Hogan V, Zhao X, Zhong Y, Han X, Wang PM, Lo YK, Li Y, Patel HA, Huang Y, Sambangi A, Chu JSV, Liu W, Fagiolini M, and Fang H

Subjects
Animals, Calcium analysis, Dielectric Spectroscopy instrumentation, Dielectric Spectroscopy methods, Electrodes, Implanted, Electrophysiology methods, Gold chemistry, Male, Mice, Inbred C57BL, Molecular Imaging, Photic Stimulation, Photons, Polystyrenes chemistry, Thiophenes chemistry, Visual Cortex diagnostic imaging, Visual Cortex physiology, Wireless Technology, Brain diagnostic imaging, Brain physiology, Electrophysiology instrumentation, Microelectrodes, Nanostructures chemistry
Abstract

Transparent microelectrode arrays have emerged as increasingly important tools for neuroscience by allowing simultaneous coupling of big and time-resolved electrophysiology data with optically measured, spatially and type resolved single neuron activity. Scaling down transparent electrodes to the length scale of a single neuron is challenging since conventional transparent conductors are limited by their capacitive electrode/electrolyte interface. In this study, we establish transparent microelectrode arrays with high performance, great biocompatibility, and comprehensive in vivo validations from a recently developed, bilayer-nanomesh material composite, where a metal layer and a low-impedance faradaic interfacial layer are stacked reliably together in a same transparent nanomesh pattern. Specifically, flexible arrays from 32 bilayer-nanomesh microelectrodes demonstrated near-unity yield with high uniformity, excellent biocompatibility, and great compatibility with state-of-the-art wireless recording and real-time artifact rejection system. The electrodes are highly scalable, with 130 kilohms at 1 kHz at 20 μm in diameter, comparable to the performance of microelectrodes in nontransparent Michigan arrays. The highly transparent, bilayer-nanomesh microelectrode arrays allowed in vivo two-photon imaging of single neurons in layer 2/3 of the visual cortex of awake mice, along with high-fidelity, simultaneous electrical recordings of visual-evoked activity, both in the multi-unit activity band and at lower frequencies by measuring the visual-evoked potential in the time domain. Together, these advances reveal the great potential of transparent arrays from bilayer-nanomesh microelectrodes for a broad range of utility in neuroscience and medical practices.

Published
2018
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2. Transcribed enhancers lead waves of coordinated transcription in transitioning mammalian cells.

Author

Arner E, Daub CO, Vitting-Seerup K, Andersson R, Lilje B, Drabløs F, Lennartsson A, Rönnerblad M, Hrydziuszko O, Vitezic M, Freeman TC, Alhendi AM, Arner P, Axton R, Baillie JK, Beckhouse A, Bodega B, Briggs J, Brombacher F, Davis M, Detmar M, Ehrlund A, Endoh M, Eslami A, Fagiolini M, Fairbairn L, Faulkner GJ, Ferrai C, Fisher ME, Forrester L, Goldowitz D, Guler R, Ha T, Hara M, Herlyn M, Ikawa T, Kai C, Kawamoto H, Khachigian LM, Klinken SP, Kojima S, Koseki H, Klein S, Mejhert N, Miyaguchi K, Mizuno Y, Morimoto M, Morris KJ, Mummery C, Nakachi Y, Ogishima S, Okada-Hatakeyama M, Okazaki Y, Orlando V, Ovchinnikov D, Passier R, Patrikakis M, Pombo A, Qin XY, Roy S, Sato H, Savvi S, Saxena A, Schwegmann A, Sugiyama D, Swoboda R, Tanaka H, Tomoiu A, Winteringham LN, Wolvetang E, Yanagi-Mizuochi C, Yoneda M, Zabierowski S, Zhang P, Abugessaisa I, Bertin N, Diehl AD, Fukuda S, Furuno M, Harshbarger J, Hasegawa A, Hori F, Ishikawa-Kato S, Ishizu Y, Itoh M, Kawashima T, Kojima M, Kondo N, Lizio M, Meehan TF, Mungall CJ, Murata M, Nishiyori-Sueki H, Sahin S, Nagao-Sato S, Severin J, de Hoon MJ, Kawai J, Kasukawa T, Lassmann T, Suzuki H, Kawaji H, Summers KM, Wells C, Hume DA, Forrest AR, Sandelin A, Carninci P, and Hayashizaki Y

Subjects
Animals, Binding Sites, Cattle, Dogs, Mice, RNA, Messenger genetics, RNA, Messenger metabolism, Rats, Stem Cells metabolism, Cell Differentiation genetics, Enhancer Elements, Genetic, Gene Expression Regulation, Developmental, Stem Cells cytology, Transcription Factors metabolism, Transcription, Genetic
Abstract

Although it is generally accepted that cellular differentiation requires changes to transcriptional networks, dynamic regulation of promoters and enhancers at specific sets of genes has not been previously studied en masse. Exploiting the fact that active promoters and enhancers are transcribed, we simultaneously measured their activity in 19 human and 14 mouse time courses covering a wide range of cell types and biological stimuli. Enhancer RNAs, then messenger RNAs encoding transcription factors, dominated the earliest responses. Binding sites for key lineage transcription factors were simultaneously overrepresented in enhancers and promoters active in each cellular system. Our data support a highly generalizable model in which enhancer transcription is the earliest event in successive waves of transcriptional change during cellular differentiation or activation., (Copyright © 2015, American Association for the Advancement of Science.)

Published
2015
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3. Observation of pulsed gamma-rays above 25 GeV from the Crab pulsar with MAGIC.

Author

Aliu E, Anderhub H, Antonelli LA, Antoranz P, Backes M, Baixeras C, Barrio JA, Bartko H, Bastieri D, Becker JK, Bednarek W, Berger K, Bernardini E, Bigongiari C, Biland A, Bock RK, Bonnoli G, Bordas P, Bosch-Ramon V, Bretz T, Britvitch I, Camara M, Carmona E, Chilingarian A, Commichau S, Contreras JL, Cortina J, Costado MT, Covino S, Curtef V, Dazzi F, De Angelis A, De Cea Del Pozo E, de Los Reyes R, De Lotto B, De Maria M, De Sabata F, Delgado Mendez C, Dominguez A, Dorner D, Doro M, Elsässer D, Errando M, Fagiolini M, Ferenc D, Fernandez E, Firpo R, Fonseca MV, Font L, Galante N, Garcia Lopez RJ, Garczarczyk M, Gaug M, Goebel F, Hadasch D, Hayashida M, Herrero A, Höhne D, Hose J, Hsu CC, Huber S, Jogler T, Kranich D, La Barbera A, Laille A, Leonardo E, Lindfors E, Lombardi S, Longo F, Lopez M, Lorenz E, Majumdar P, Maneva G, Mankuzhiyil N, Mannheim K, Maraschi L, Mariotti M, Martinez M, Mazin D, Meucci M, Meyer M, Miranda JM, Mirzoyan R, Moles M, Moralejo A, Nieto D, Nilsson K, Ninkovic J, Otte N, Oya I, Paoletti R, Paredes JM, Pasanen M, Pascoli D, Pauss F, Pegna RG, Perez-Torres MA, Persic M, Peruzzo L, Piccioli A, Prada F, Prandini E, Puchades N, Raymers A, Rhode W, Ribó M, Rico J, Rissi M, Robert A, Rügamer S, Saggion A, Saito TY, Salvati M, Sanchez-Conde M, Sartori P, Satalecka K, Scalzotto V, Scapin V, Schweizer T, Shayduk M, Shinozaki K, Shore SN, Sidro N, Sierpowska-Bartosik A, Sillanpää A, Sobczynska D, Spanier F, Stamerra A, Stark LS, Takalo L, Tavecchio F, Temnikov P, Tescaro D, Teshima M, Tluczykont M, Torres DF, Turini N, Vankov H, Venturini A, Vitale V, Wagner RM, Wittek W, Zabalza V, Zandanel F, Zanin R, Zapatero J, de Jager OC, and de Ona Wilhelmi E

Abstract

One fundamental question about pulsars concerns the mechanism of their pulsed electromagnetic emission. Measuring the high-end region of a pulsar's spectrum would shed light on this question. By developing a new electronic trigger, we lowered the threshold of the Major Atmospheric gamma-ray Imaging Cherenkov (MAGIC) telescope to 25 giga-electron volts. In this configuration, we detected pulsed gamma-rays from the Crab pulsar that were greater than 25 giga-electron volts, revealing a relatively high cutoff energy in the phase-averaged spectrum. This indicates that the emission occurs far out in the magnetosphere, hence excluding the polar-cap scenario as a possible explanation of our measurement. The high cutoff energy also challenges the slot-gap scenario.

Published
2008
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4. Very-high-energy gamma rays from a distant quasar: how transparent is the universe?

Author

Albert J, Aliu E, Anderhub H, Antonelli LA, Antoranz P, Backes M, Baixeras C, Barrio JA, Bartko H, Bastieri D, Becker JK, Bednarek W, Berger K, Bernardini E, Bigongiari C, Biland A, Bock RK, Bonnoli G, Bordas P, Bosch-Ramon V, Bretz T, Britvitch I, Camara M, Carmona E, Chilingarian A, Commichau S, Contreras JL, Cortina J, Costado MT, Covino S, Curtef V, Dazzi F, De Angelis A, De Cea Del Pozo E, de Los Reyes R, De Lotto B, De Maria M, De Sabata F, Mendez CD, Dominguez A, Dorner D, Doro M, Errando M, Fagiolini M, Ferenc D, Fernández E, Firpo R, Fonseca MV, Font L, Galante N, López RJ, Garczarczyk M, Gaug M, Goebel F, Hayashida M, Herrero A, Höhne D, Hose J, Hsu CC, Huber S, Jogler T, Kneiske TM, Kranich D, La Barbera A, Laille A, Leonardo E, Lindfors E, Lombardi S, Longo F, López M, Lorenz E, Majumdar P, Maneva G, Mankuzhiyil N, Mannheim K, Maraschi L, Mariotti M, Martínez M, Mazin D, Meucci M, Meyer M, Miranda JM, Mirzoyan R, Mizobuchi S, Moles M, Moralejo A, Nieto D, Nilsson K, Ninkovic J, Otte N, Oya I, Panniello M, Paoletti R, Paredes JM, Pasanen M, Pascoli D, Pauss F, Pegna RG, Perez-Torres MA, Persic M, Peruzzo L, Piccioli A, Prada F, Prandini E, Puchades N, Raymers A, Rhode W, Ribó M, Rico J, Rissi M, Robert A, Rügamer S, Saggion A, Saito TY, Salvati M, Sanchez-Conde M, Sartori P, Satalecka K, Scalzotto V, Scapin V, Schmitt R, Schweizer T, Shayduk M, Shinozaki K, Shore SN, Sidro N, Sierpowska-Bartosik A, Sillanpää A, Sobczynska D, Spanier F, Stamerra A, Stark LS, Takalo L, Tavecchio F, Temnikov P, Tescaro D, Teshima M, Tluczykont M, Torres DF, Turini N, Vankov H, Venturini A, Vitale V, Wagner RM, Wittek W, Zabalza V, Zandanel F, Zanin R, and Zapatero J

Abstract

The atmospheric Cherenkov gamma-ray telescope MAGIC, designed for a low-energy threshold, has detected very-high-energy gamma rays from a giant flare of the distant Quasi-Stellar Radio Source (in short: radio quasar) 3C 279, at a distance of more than 5 billion light-years (a redshift of 0.536). No quasar has been observed previously in very-high-energy gamma radiation, and this is also the most distant object detected emitting gamma rays above 50 gigaelectron volts. Because high-energy gamma rays may be stopped by interacting with the diffuse background light in the universe, the observations by MAGIC imply a low amount for such light, consistent with that known from galaxy counts.

Published
2008
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5. Variable very-high-energy gamma-ray emission from the microquasar LS I +61 303.

Author

Albert J, Aliu E, Anderhub H, Antoranz P, Armada A, Asensio M, Baixeras C, Barrio JA, Bartelt M, Bartko H, Bastieri D, Bavikadi SR, Bednarek W, Berger K, Bigongiari C, Biland A, Bisesi E, Bock RK, Bordas P, Bosch-Ramon V, Bretz T, Britvitch I, Camara M, Carmona E, Chilingarian A, Ciprini S, Coarasa JA, Commichau S, Contreras JL, Cortina J, Curtef V, Danielyan V, Dazzi F, De Angelis A, de Los Reyes R, De Lotto B, Domingo-Santamaría E, Dorner D, Doro M, Errando M, Fagiolini M, Ferenc D, Fernández E, Firpo R, Flix J, Fonseca MV, Font L, Fuchs M, Galante N, Garczarczyk M, Gaug M, Giller M, Goebel F, Hakobyan D, Hayashida M, Hengstebeck T, Höhne D, Hose J, Hsu CC, Isar PG, Jacon P, Kalekin O, Kosyra R, Kranich D, Laatiaoui M, Laille A, Lenisa T, Liebing P, Lindfors E, Lombardi S, Longo F, López J, López M, Lorenz E, Lucarelli F, Majumdar P, Maneva G, Mannheim K, Mansutti O, Mariotti M, Martínez M, Mase K, Mazin D, Merck C, Meucci M, Meyer M, Miranda JM, Mirzoyan R, Mizobuchi S, Moralejo A, Nilsson K, Oña-Wilhelmi E, Orduña R, Otte N, Oya I, Paneque D, Paoletti R, Paredes JM, Pasanen M, Pascoli D, Pauss F, Pavel N, Pegna R, Persic M, Peruzzo L, Piccioli A, Poller M, Pooley G, Prandini E, Raymers A, Rhode W, Ribó M, Rico J, Riegel B, Rissi M, Robert A, Romero GE, Rügamer S, Saggion A, Sánchez A, Sartori P, Scalzotto V, Scapin V, Schmitt R, Schweizer T, Shayduk M, Shinozaki K, Shore SN, Sidro N, Sillanpää A, Sobczynska D, Stamerra A, Stark LS, Takalo L, Temnikov P, Tescaro D, Teshima M, Tonello N, Torres A, Torres DF, Turini N, Vankov H, Vitale V, Wagner RM, Wibig T, Wittek W, Zanin R, and Zapatero J

Abstract

Microquasars are binary star systems with relativistic radio-emitting jets. They are potential sources of cosmic rays and can be used to elucidate the physics of relativistic jets. We report the detection of variable gamma-ray emission above 100 gigaelectron volts from the microquasar LS I 61 + 303. Six orbital cycles were recorded. Several detections occur at a similar orbital phase, which suggests that the emission is periodic. The strongest gamma-ray emission is not observed when the two stars are closest to one another, implying a strong orbital modulation of the emission or absorption processes.

Published
2006
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6. The transcriptional landscape of the mammalian genome.

Author

Carninci P, Kasukawa T, Katayama S, Gough J, Frith MC, Maeda N, Oyama R, Ravasi T, Lenhard B, Wells C, Kodzius R, Shimokawa K, Bajic VB, Brenner SE, Batalov S, Forrest AR, Zavolan M, Davis MJ, Wilming LG, Aidinis V, Allen JE, Ambesi-Impiombato A, Apweiler R, Aturaliya RN, Bailey TL, Bansal M, Baxter L, Beisel KW, Bersano T, Bono H, Chalk AM, Chiu KP, Choudhary V, Christoffels A, Clutterbuck DR, Crowe ML, Dalla E, Dalrymple BP, de Bono B, Della Gatta G, di Bernardo D, Down T, Engstrom P, Fagiolini M, Faulkner G, Fletcher CF, Fukushima T, Furuno M, Futaki S, Gariboldi M, Georgii-Hemming P, Gingeras TR, Gojobori T, Green RE, Gustincich S, Harbers M, Hayashi Y, Hensch TK, Hirokawa N, Hill D, Huminiecki L, Iacono M, Ikeo K, Iwama A, Ishikawa T, Jakt M, Kanapin A, Katoh M, Kawasawa Y, Kelso J, Kitamura H, Kitano H, Kollias G, Krishnan SP, Kruger A, Kummerfeld SK, Kurochkin IV, Lareau LF, Lazarevic D, Lipovich L, Liu J, Liuni S, McWilliam S, Madan Babu M, Madera M, Marchionni L, Matsuda H, Matsuzawa S, Miki H, Mignone F, Miyake S, Morris K, Mottagui-Tabar S, Mulder N, Nakano N, Nakauchi H, Ng P, Nilsson R, Nishiguchi S, Nishikawa S, Nori F, Ohara O, Okazaki Y, Orlando V, Pang KC, Pavan WJ, Pavesi G, Pesole G, Petrovsky N, Piazza S, Reed J, Reid JF, Ring BZ, Ringwald M, Rost B, Ruan Y, Salzberg SL, Sandelin A, Schneider C, Schönbach C, Sekiguchi K, Semple CA, Seno S, Sessa L, Sheng Y, Shibata Y, Shimada H, Shimada K, Silva D, Sinclair B, Sperling S, Stupka E, Sugiura K, Sultana R, Takenaka Y, Taki K, Tammoja K, Tan SL, Tang S, Taylor MS, Tegner J, Teichmann SA, Ueda HR, van Nimwegen E, Verardo R, Wei CL, Yagi K, Yamanishi H, Zabarovsky E, Zhu S, Zimmer A, Hide W, Bult C, Grimmond SM, Teasdale RD, Liu ET, Brusic V, Quackenbush J, Wahlestedt C, Mattick JS, Hume DA, Kai C, Sasaki D, Tomaru Y, Fukuda S, Kanamori-Katayama M, Suzuki M, Aoki J, Arakawa T, Iida J, Imamura K, Itoh M, Kato T, Kawaji H, Kawagashira N, Kawashima T, Kojima M, Kondo S, Konno H, Nakano K, Ninomiya N, Nishio T, Okada M, Plessy C, Shibata K, Shiraki T, Suzuki S, Tagami M, Waki K, Watahiki A, Okamura-Oho Y, Suzuki H, Kawai J, and Hayashizaki Y

Subjects
3' Untranslated Regions, Animals, Base Sequence, Conserved Sequence, DNA, Complementary chemistry, Genome, Human, Genomics, Humans, Promoter Regions, Genetic, Proteins genetics, RNA chemistry, RNA classification, RNA Splicing, RNA, Untranslated chemistry, Regulatory Sequences, Ribonucleic Acid, Genome, Mice genetics, Terminator Regions, Genetic, Transcription Initiation Site, Transcription, Genetic
Abstract

This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development.

Published
2005
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7. Specific GABAA circuits for visual cortical plasticity.

Author

Fagiolini M, Fritschy JM, Löw K, Möhler H, Rudolph U, and Hensch TK

Subjects
Animals, Diazepam pharmacology, Mice, Mice, Inbred C57BL, Models, Neurological, Mutation, Neural Inhibition, Protein Subunits, Pyridines pharmacology, Receptors, GABA-A genetics, Synaptic Transmission, Vision, Ocular, Visual Cortex growth & development, Zolpidem, gamma-Aminobutyric Acid physiology, Dominance, Ocular physiology, Interneurons physiology, Neuronal Plasticity, Neurons physiology, Receptors, GABA-A physiology, Visual Cortex physiology
Abstract

Weak inhibition within visual cortex early in life prevents experience-dependent plasticity. Loss of responsiveness to an eye deprived of vision can be initiated prematurely by enhancing gamma-aminobutyric acid (GABA)-mediated transmission with benzodiazepines. Here, we use a mouse "knockin" mutation to alpha subunits that renders individual GABA type A (GABA(A)) receptors insensitive to diazepam to show that a particular inhibitory network controls expression of the critical period. Only alpha1-containing circuits were found to drive cortical plasticity, whereas alpha2-enriched connections separately regulated neuronal firing. This dissociation carries implications for models of brain development and the safe design of benzodiazepines for use in infants.

Published
2004
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8. Local GABA circuit control of experience-dependent plasticity in developing visual cortex.

Author

Hensch TK, Fagiolini M, Mataga N, Stryker MP, Baekkeskov S, and Kash SF

Subjects
Animals, Diazepam pharmacology, GABA Modulators pharmacology, Gene Targeting, Glutamate Decarboxylase genetics, Long-Term Potentiation, Mice, Mice, Inbred C57BL, Mice, Knockout, Photic Stimulation, Receptors, GABA-A metabolism, Synaptic Transmission, Visual Cortex cytology, Visual Cortex metabolism, Visual Pathways, Glutamate Decarboxylase metabolism, Interneurons physiology, Neuronal Plasticity drug effects, Visual Cortex physiology, gamma-Aminobutyric Acid metabolism
Abstract

Sensory experience in early life shapes the mammalian brain. An impairment in the activity-dependent refinement of functional connections within developing visual cortex was identified here in a mouse model. Gene-targeted disruption of one isoform of glutamic acid decarboxylase prevented the competitive loss of responsiveness to an eye briefly deprived of vision, without affecting cooperative mechanisms of synapse modification in vitro. Selective, use-dependent enhancement of fast intracortical inhibitory transmission with benzodiazepines restored plasticity in vivo, rescuing the genetic defect. Specific networks of inhibitory interneurons intrinsic to visual cortex may detect perturbations in sensory input to drive experience-dependent plasticity during development.

Published
1998
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