Search

DNA solutions subjected to an electric field exhibit an instability that leads to DNA segregation in aggregates tilted with regard to the field. With the use of epifluorescence videomicroscopy, the evolution of DNA patterns in capillaries as a function of DNA concentration, DNA size, field strength, and field frequency was studied. The field threshold for segregation was decreased when the frequency was lowered or when the DNA molecular weight or concentration was increased. Aggregation is attributed to an electrohydrodynamic instability triggered by the dipole-dipole interaction. This phenomenon explains the failure of earlier attempts to separate large DNA in capillaries., Molecular genetics relies heavily on the electrophoretic separation of DNA molecules by size. Until recently, this was achieved mainly in slab gels, but the joint use of capillary electrophoresis (CE) [...]

Observation of Individual DNA Molecules Undergoing Gel Electrophoresis THE ADVENT OF PULSED-FIELD GEL electrophoresis (PFGE) has allowed the separation of very large DNA fragments with relative ease, but the underlying [...]

Capillary Electrophoresis MANY MOLECULES EXIST IN SOLUTION AS CHARGED SPECIES (ions), either with positive charges (cations) or negative charges (anions). When an external electric field is applied to a solution [...]

Derivation of Clones Close to met by Preparative Field Inversion Gel Electrophoresis MAMMALIAN MOLECULAR GENETicshas long been limited to the study of genes whose products are known. This is mainly [...]

Electrophoretic Separations of Large DNA Molecules by Periodic Inversion of the Electric Field GEL ELECTROPHORESIS, IN WHICH Gions are separated by size as they migrate through a gel medium in [...]

Microcolumn Separations and the Analysis fo Single Cells IN THE DEVELOPMENT OF ANALYTICAL INSTRUMENTAL, miniaturization is often a fruitful endeavor. During the past 20 years, numerous scietific advances arising from [...]

Electrokinetic chromatography (EKC), a mode of capillary electrophoresis, is a group of analytical separation techniques named after electrokinetic phenomena that include electroosmosis, electrophoresis, and chromatography (1). EKC offers diverse advantages […]