1. A petavoxel fragment of human cerebral cortex reconstructed at nanoscale resolution.
- Author
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Shapson-Coe A, Januszewski M, Berger DR, Pope A, Wu Y, Blakely T, Schalek RL, Li PH, Wang S, Maitin-Shepard J, Karlupia N, Dorkenwald S, Sjostedt E, Leavitt L, Lee D, Troidl J, Collman F, Bailey L, Fitzmaurice A, Kar R, Field B, Wu H, Wagner-Carena J, Aley D, Lau J, Lin Z, Wei D, Pfister H, Peleg A, Jain V, and Lichtman JW
- Subjects
- Humans, Axons physiology, Axons ultrastructure, Dendrites physiology, Neurons ultrastructure, Oligodendroglia ultrastructure, Synapses physiology, Synapses ultrastructure, Temporal Lobe ultrastructure, Microscopy, Cerebral Cortex blood supply, Cerebral Cortex ultrastructure
- Abstract
To fully understand how the human brain works, knowledge of its structure at high resolution is needed. Presented here is a computationally intensive reconstruction of the ultrastructure of a cubic millimeter of human temporal cortex that was surgically removed to gain access to an underlying epileptic focus. It contains about 57,000 cells, about 230 millimeters of blood vessels, and about 150 million synapses and comprises 1.4 petabytes. Our analysis showed that glia outnumber neurons 2:1, oligodendrocytes were the most common cell, deep layer excitatory neurons could be classified on the basis of dendritic orientation, and among thousands of weak connections to each neuron, there exist rare powerful axonal inputs of up to 50 synapses. Further studies using this resource may bring valuable insights into the mysteries of the human brain.
- Published
- 2024
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