1. Characterization of a unique cell line (LAZ 221) from human acute lymphocytic ("null" cell) leukemia.
- Author
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Lazarus H, Barell EF, Krishan A, Livingston DM, Harris K, Schlossman SF, and Chess L
- Subjects
- Adult, Antigens, Neoplasm, Cell Line, Cell Membrane immunology, Chromosome Aberrations, Chromosomes, Human, 6-12 and X, Female, Herpesvirus 4, Human immunology, Humans, Isoantigens, Leukemia, Lymphoid genetics, Leukemia, Lymphoid pathology, Neoplasms, Experimental immunology, Leukemia, Lymphoid immunology, Lymphocytes immunology
- Abstract
A unique human cell line designated LAZ 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the "null" cell type. The cell line does not possess the Epstein-Barr virus nuclear antigen and has a karyotype of 45,XX,-9,-12,+(9q12q). Both the established cell line and the patient's uncultured blast cells share the same phenotypic markers. They both lack T-cell markers. They fail to form sheep erythrocyte rosettes and do not react with T-cell-specific antisera (TH1-, HTL-), nor do they possess B-cell markers. They do not form rosettes with erythrocytes sensitized with complement, and they are surface immunoglobulin negative. However, they do possess an HLA-D-related glycoprotein complex of 23,000 to 30,000 daltons, an la-like antigen. Thus, LAZ 221 shares the phenotype of the patient's uncultured blasts and is a cell line representative of about 75% of all human acute lymphocytic leukemias. In this respect it differs from previously described human hematopoietic cell lines.
- Published
- 1978