1. Gene Expression of Nucleic Acid-Sensing Pattern Recognition Receptors in Children Hospitalized for Respiratory Syncytial Virus-Associated Acute Bronchiolitis
- Author
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Carla Selvaggi, Enea Bonci, Alessandra Pierangeli, Carolina Scagnolari, Paola Di Marco, Fabio Midulla, Corrado Moretti, Rosaria Berardi, Guido Antonelli, Daniela De Angelis, and Enrico Girardi
- Subjects
Male ,Microbiology (medical) ,viruses ,Clinical Biochemistry ,Immunology ,Respiratory Syncytial Virus Infections ,Biology ,Severity of Illness Index ,Virus ,Interferon ,Nasopharynx ,medicine ,Humans ,Immunology and Allergy ,Innate immune system ,Gene Expression Profiling ,Pattern recognition receptor ,Infant ,Length of Stay ,medicine.disease ,Acquired immune system ,Virology ,Hospitalization ,Gene expression profiling ,Bronchiolitis ,Receptors, Pattern Recognition ,Respiratory Syncytial Virus, Human ,Female ,Interferons ,Microbial Immunology ,Viral load ,medicine.drug - Abstract
Given the critical role of pattern recognition receptors (PRRs) in acid nucleic recognition in the initiation of innate immunity and the orchestration of adaptive immunity, the aim of this study was to determine whether any heterogeneity of PRR expression in the airway tracts of infants with respiratory syncytial virus (RSV) infection might explain the broad clinical spectrum of RSV-associated bronchiolitis in infants. For this purpose, the levels of melanoma differentiation-associated protein-5 (MDA-5), retinoic acid inducible gene-1 (RIG-1), and Toll-like receptor 3 (TLR-3), TLR-7, TLR-8, and TLR-9 mRNAs were evaluated, using TaqMan quantitative reverse transcription-PCR, in cells from nasopharyngeal washes collected from 157 infants suffering from acute bronchiolitis whether or not they were associated with respiratory viruses. High interindividual variability was observed in both virus-positive and -negative infants; however, the relative gene expression levels of MDA-5, RIG-1, TLR-7, and TLR-8 were significantly higher in the virus-infected group, whereas the expression levels of TLR-3 and TLR-9 were not significantly different. The differences in the gene expression of MDA-5, RIG-1, TLR-7, and TLR-8 were more evident in infants with RSV infection than in those with bocavirus or rhinovirus infection. In RSV-infected infants, PRR-mRNA levels also were analyzed in relation to interferon protein levels, viral load, clinical severity, days of hospitalization, age, and body weight. A significant positive correlation was observed only between RSV viral load and RIG-1 mRNA levels. These findings provide the first direct evidence that, in infants with respiratory virus-associated bronchiolitis, especially RSV, there are substantial changes in PRR gene expression; this likely is an important determinant of the clinical outcome of bronchiolitis.
- Published
- 2009