1. Deciphering The Glycosylome Of Dystroglycanopathies Using Haploid Screens For Lassa Virus Entry
- Author
-
Matthijs Raaben, Sean P. J. Whelan, Peter Meinecke, Marja W. Wessels, Dirk Lefeber, Hans van Bokhoven, Ellen van Beusekom, Arno Velds, Thijn R. Brummelkamp, Haluk Topaloglu, Ron M. Kerkhoven, Vincent A. Blomen, Moniek Riemersma, Lucas T. Jae, Jan E. Carette, Çocuk Sağlığı ve Hastalıkları, and Clinical Genetics
- Subjects
Male ,Glycosylation ,Proteome ,Haploidy ,medicine.disease_cause ,chemistry.chemical_compound ,0302 clinical medicine ,Dystroglycans ,Lassa fever ,Genetics ,0303 health sciences ,Mutation ,Multidisciplinary ,biology ,Walker-Warburg Syndrome ,Pedigree ,3. Good health ,Host-Pathogen Interactions ,Science & Technology - Other Topics ,Female ,lipids (amino acids, peptides, and proteins) ,musculoskeletal diseases ,Glycan ,DCN MP - Plasticity and memory ,Molecular Sequence Data ,Article ,Cell Line ,03 medical and health sciences ,Lassa Fever ,medicine ,Humans ,Amino Acid Sequence ,Pentosyltransferases ,Lassa virus ,Glycostation disorders [DCN PAC - Perception action and control IGMD 4] ,Gene ,DCN NN - Brain networks and neuronal communication ,030304 developmental biology ,Infant ,Membrane Proteins ,Virus Internalization ,Glycostation disorders [IGMD 4] ,medicine.disease ,Virology ,Genetics and epigenetic pathways of disease DCN MP - Plasticity and memory [NCMLS 6] ,carbohydrates (lipids) ,Membrane protein ,chemistry ,biology.protein ,030217 neurology & neurosurgery - Abstract
Viruses and Congenital Disorders Mutations in genes involved in α-dystroglycan O-linked glycosylation result in posttranslation modifications associated with the congenital disease Walker-Warburg syndrome (WWS). This cellular modification is also required for efficient Lassa virus infection of cells. Jae et al. (p. 479 , published online 21 March) screened for genes involved in O-glycosylation that affected Lassa virus infection and identified candidates involved in glycosylation. Individuals from different pedigrees exhibiting WWS had unique mutations among genes identified in the genetic screen. Thus, comprehensive forward genetic screens can be used to define the genetic architecture of a complex disease.
- Published
- 2013