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The present work addresses the optimization of temperature-increasing, environmentally friendly biodegradable film with titanium dioxide and rice straw fiber using response surface methodology (RSM) analysis. The effects of the various process parameters (basis weight, neutral sizing agent, addition ratio of titanium dioxide, wet strength agent, and beating degree) on the dry tensile strength, wet tensile strength, and light-scattering coefficient of the fiber film are reported. The optimal technical parameters of the temperature-increasing film made from titanium dioxide and rice straw fiber included a 64 to 68 g/m2 basis weight, 1.55% to 1.60% wet strength agent, 0.9% neutral sizing agent, 18% addition ratio of titanium dioxide, and 35°SR beating degree. Under this condition, the dry tensile strength, wet tensile strength, and light-scattering coefficient were higher than 30 N, 10 N, and 45 m2/kg, respectively. The film was characterized using Fourier transform infrared (FTIR) spectroscopy. In addition, the resulting film met the requirements of mechanical properties for field mulching and agronomic requirements for crop growth. The results provided a reference for the preparation of fiber film suitable for the temperature required for crop growth.