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To evaluate the bacteriological contamination of groundwater wells due to onsite sanitation in Jempol coast, a survey was conducted in order to measure the concentration of E. coli, and fecal coliform indicators, as well as the presence of viruses, generally infect the human. Groundwater specimens were collected from twenty-five wells in Jempol coast and were analyzed for the presence of E. coli and fecal coliform. Dominantly, bacteriological analysis results were in accordance with the Indonesian standards based on Permenkes RI no no 416/MENKES/PER/XI/1990 Occasionally, bacteriological contaminations were found in all sites. E. coli were found in all samples, respectively. Twenty-one samples (84%) were positive contaminated by Escherichia coli, and nineteen samples (76%) were positive contaminated by Faecal coliform. The overall data showed that groundwater well is impacted by human viruses. Both bacterial as indicators of pollution that have high predictive capability for the presence of other human viruses in a groundwater well. The distance of onsite sanitation analysed was closely related to the presence of the bacterial indicator and viruses. Appropriate and effective actions must be taken to improve water quality and reduce public health risk.