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The relative content of biologically active substances in the extracts of wood decaying fungi was studied using GC-MS. The results showed that the real tinder (Fomes fomentarius (L.) Fr.) contains up to 18% fatty acids, 51.6% steroids and 8.35% triterpene compounds. The nest fungus (Hapalopilus nidulans (Fr.) P. Karst.) contains 32.7% of fatty acids, 9.8% of steroid substances and 27.0% of tetracyclic triterpenoids. Chaga (Inonotus obliguus (Ach. ex. Pers.) Pilat) is characterized by the presence of 14% fatty acids, 11.6% of which are unsaturated; 2.4% of ergosterol, and 30% of betulin. A distinctive feature of the extract from the birch sponge (Piptoporus betulinus (Bull.) P. Karst.) is the presence of 67.9% arabitol, and the absence of steroid and triterpene compounds. Bordered tinder fungus (Fomitopsis pinicola (Sw.) P. Karst.), causing brown rot of wood, contains 30.15% ergosterol, which is a provitamin form of vitamin D2. The tinder fungus extract (Trichaptum biforme (Fr.) Ryvarden) contains 67.8% fatty acids, of which 50.0% are unsaturated fatty acids. The extract from the humpback tinder fungus (Trametes gibbosa (Pers.) Fr.) contains 34.9% fatty acids, 9.76% steroids, and 27.06% terpenoid compounds. © 2022 Author(s).