1. Rapid detection of urokinase plasminogen activator using flexible paper-based graphene-gold platform.
- Author
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Sharma B, Parajuli P, and Podila R
- Subjects
- Antibodies chemistry, Antibodies immunology, Biosensing Techniques methods, Density Functional Theory, Humans, Immunoassay, Limit of Detection, Neoplasms diagnosis, Recombinant Proteins analysis, Recombinant Proteins biosynthesis, Recombinant Proteins genetics, Urokinase-Type Plasminogen Activator immunology, Gold chemistry, Graphite chemistry, Metal Nanoparticles chemistry, Paper, Urokinase-Type Plasminogen Activator analysis
- Abstract
Many studies have shown that urokinase plasminogen activator (uPA) is causally involved in promoting cancer invasion and metastasis. Thus, monitoring uPA levels could be very useful in cancer diagnosis, identification of initial metastasis, and guiding cancer treatment. Here, the authors developed a novel and scalable uPA sensor based on a graphene-gold nanoparticle platform that uses fluorescence of quantum dots to rapidly (<1 h) detect uPA up to 100 pM. Indeed, the authors' sensor is highly selective and showed an ability to sense up to 100 pM uPA even in the presence of complex biological milieu such as the fetal bovine serum.
- Published
- 2020
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