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Surface‐Enhanced Raman Scattering (SERS) of amino acids has proven a challenging task. The SERS of biomolecules and the formation of a reliable SERS data‐base for medical application has shown to be a very challenging task for two reasons: first, the functional groups in proteins, lipid and sugars do not usually include aromatic moieties or chromophores absorbing in the visible (with intrinsic very large cross sections), and therefore, large enhancement factors are needed for ultrasensitive analysis. Second, the biomolecule‐metal surface interaction leads to active surface photochemistry, photoisomerization or photodissociation, thus hindering the reproducibility of the experiment.In this work, we have employed hydroxyproline (Hyp) as our problem molecule, and tackled the problem of reproducibility by employing gold colloids instead of the usual silver to achieve SERS. We slightly modified the procedure described by Lee and Meisel to obtain the colloid, and concentrated the gold particles by centrifuging ...