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In the Dognecea Lake, near town Bocsa in Caras-Severin county a strong bivalve population has been discovered from the duck mussel specie (Anodonta anatina). Harvested individuals were transported to the Aquaculture laboratory where measurements and correct identification was made. This specie is considered endangered but in Banat area, especially in the Dognecea Lake it is well represented, having an ecological importance, but the bivalve can have an economical importance too, due to the high percentage of edible part of 56.5%.

In the present study we calculated the correlations between the “alive” (green) weight and the biometric parameters of the shell according to the geographical area and the metabolic activity status. The biological material studied belongs to the species Helix pomatia. var. Banatica. from two different areas: Pădurea Verde and OraviŃa. At both studied populations. the measurements and weighing were made depending on the metabolic activity status (with operculum or without operculum). From the analysis of the correlations results that the “alive” weight has a large positive correlation with the foot weight and the depth of the shell. in both snails populations. regardless of the metabolic activity status (r > 0.5). In the case of snails without operculum. all the biometrical parameters of the shell have a large positive correlation with the “alive” weight (values between 0.50016 and 0.989085). Negative correlations have been observed in the case of the snails with operculum. between the alive weight and the shell height (r = 0.00231). and between the alive weight and shell width (r = 0.00443).

Non additive genetic component that is the base of a quantitative character has no predictability, this is the reason for which the present paper is proposing to appreciate the special combinatory capacity for corporal mass of the descendants at birth in a specific crossing scheme combining three rabbit breeds common for total gain and average daily gain the finishing period (30- 80 days), in Timis county breeders New Zealand White, Large Chinchilla and Californian. The data obtained, statistic presented prove a good special combinative capacity for this character, reason for which the crossing scheme is recommended for producing meat individuals destined for slaughterhouses. This scheme provides an exploitation of the high fervency of the heterozygote at maternal forms and at the final hybrids.

Electroporation is used to induce homologous recombination in the genome of the murine ES (embryonic stem) cells. Routinelly subconfluent ES cells are recommended to be used in such experiments. Electroporation of immunoglobulin specific targeting vectors with different length of homology leads to reduced number of selected colonies. The enrichment of double selected colonies is high and thus the amount of HM-1 ES cell colonies for the analysis is very low. Here we show that the electroporation of confluent HM-1 ES cells leads to an increased amount of simple and double selected colonies.

The efficiency of homologous recombination relies on the lenght of the homology arms and the chromatin compactation of the locus. In ES cells the immuoglobulin locu is compacted. Thus, the homologous recombination events occur at a very low frequency. A high number of ES cell colonies have to be analysed to detect a homologous recombination event. Here we describe a reliable and rapid protocol for the analysis of targeted mutagenesis in the immunoglobulin locus using Southern Blot analysis.

The paper presents the results of the research concerning chemical composition of acacia honey from the differents region. Analisys concerning the mineral content were made in 14-19 march 2008 in the molecular and atomical spectroscopy laboratoriy of the Faculty of Agro-Food Tehnology and Processing from USABMV Timisoara, through spectroscopy with atomic absorbability in flame with the spectrometer. Contr AA 300 with continous surce. There were anailzted 3 honey samples made in 2007 from Domasnea and Farliug (Caras-Severin) and Bala (Mehedinti). The ash contain in Na, K, Ca, Mg, Cu, Zn, Mn, Fe, Pb, Co, Cr was determined.

In rabbits breeding, the amelioration processes have a high importance because they are aimed to continuously increase the productions concomitantly with the decrease of specific consumption and unit costs. Cross-breeding of two genetically distinguished breeds can produce the heterozis effect, meaning an increase of the possibility that allow a higher productivity. 53 young rabbits were used as biological material, 3 interlinear simple female hybrids of New Zealand White (NZ) being the maternal line and 3 interlinear simple male hybrids of Grand Chinchilla (CH) form the paternal line. The 53 young rabbits were raised in identical environmental conditions so that their genetic potential to determinate the phonotypical expression. The rabbits weighing was done daily, at the same time, in equal foraging and drinking conditions. The rabbits were weaned at 30 days old, and until 80 days old were raised for meat. The two breeds used in the crossbreeding chart, respectively New Zealand White as maternal line and Grand Chinchilla as paternal line, have a good combinative characteristic, and on their hybrids is manifested the heterozis effect. In all the experimental, the hybrids from NZ x CH cross-breeding registered a corporal dynamic higher then the parental breeds.

The study was made in 2007 on 500 females of Chinchilla,kept in individual cages, with a passage for the male. The next parameters were established based on reproduction results: prolificity index (PI), sex ratio, offspring survival index (OSI), female usage index (FUI). Based on these parameters, the fertility index has been calculated,during two births. For the first birth, the fertility index was 4.22 offsprings/female, for the second birth, it was 3.65 offsprings/female.

Precious biological characteristics of rabbits make their breeding a very profitable occupation. The rabbit meat, organoleptically same to the white meat, is rich in proteins, but low in fats. Biological researched done in direction to elucidate the biochemical systems that are the basis for organism physiological processes, have revealed that the level in which this process are develop directly influence the rabbits productivity capacity. 60 rabbit’s heads was used as biological material, distributed in: 15 Californian, 15 New Zeeland White, 15 Grand Chinchilla and 15 F1NZCH hybrids obtained from cross-breeding the New Zeeland White as maternal form and Grand Chinchilla as paternal form. Blood was sampled from the rabbit and was biochemical analyzed. The studied indices were: total protein, albumin, urea, uric acid, creatinine, total bilirubine, cholesterol, triglyceride and glucose. The experimental lot formed from F1 NZCH hybrids registered a concentration of 2.1 mg/dl uric acid, and in the other three lots the concentration was under 2 mg/dl. In all four lots, uric acid value was in normal limits. The determined creatinine registered very low values, under 1 mg/dl, at the low limit of reference values. At hybrids from New Zeeland White as maternal form and Grand Chinchilla as paternal form, in equal environmental conditions, the serum biochemical analysis haven’t registered significant differences compared to pure breeds individuals.

With the help of body measurements we can appreciate the general development of fishes, the interdependence between different body regions, the growth rhythm, conformation and data use for relative and absolute values. Analyzing the average of female and male total body length it was noticed that at females the mean body length (9.99 ± 0.2 cm) is higher that in males (9.0 9 ± 0.23 cm). Mean body weight at females was 9.77 ± 0.71, and in males was 6.97 g ± 0.49. Maximum mean height in females was 2.41 ± 0.06 cm and in males was 2.08 ± 0.05 cm. Minimum female body height was 0.82 cm while at males was 0.73 cm.

The aim of this paper was to measure the main aspects that characterize the rumination behavior in six months old calves fed using three different feed spaces allowance. During the experiments the following rumination behavior aspects were determined: number of rumination periods, the length of rumination periods and the total length of rumination periods. Results showed that calves spent ruminating, on average, only 96.4 minutes in the morning, 176 minutes in the afternoon and 262.6 minutes during the night when the length of feeding space was 0.6 m per calf. Calves spent ruminating 124.2 minutes during the morning, 173.6 minutes during the afternoon and 243.4 minutes during the night when the length of the feeding space was 0.32 m per calf. Calves spent ruminating 106.2 minutes during the morning, 134.2 minutes during the afternoon and 286.4 minutes during the night when the length of the feeding space was 0.28 m per calf. The total time spent ruminating by calves was 535 minutes when the length of feeding space was 0.6 m per calf, 541 minutes when the length of feeding space was 0.32 per calf and 527 minutes when the length of the feeding space was 0.28 per calf. There were no significant differences (p>0.05) for total time spent ruminating by calves between the three experimental variants.

On this research is showing the present situation of the fish genostock in the Nadrag River, an important confluent of the Timis river, being part of the Banat hydrographical area. The fish species caught in the investigated area are part of Cyprinidae family. The dominant species is Barbus meridionalis petenyi, followed by Alburnoides bipunctatus, incorporating the investigated area in the European grayling (Thymallus thymallus) or the Mediterranean barbel (Barbus meridionalis petenyi) zone of the running waters. At present the Barbus meridionalis petentyi population from the area investigated founds itself in a regression, fact that can be connected both with the poaching and with unauthorized hydrotechnic buildings build-up in the last decade.

The paper prezents a study on the effect of acidifying substances on the development the bee families during spring. The research was made at beeyards from Jebel, Timis county from 15 April to 4 May 2011. The biological material being represented by 30 bee families of Apis mellifica carpatica, Banat ecotype, divided in 3 experimental grups of 10 families each with equal strength. The bee families of experimental grups were fed sugar syrup where incorporated lactic or acetic acid. During the research period was analized the number of brood cells at 7, 14, 21 days. Experimental groups that were fed acidifying substances recorded statistical differences in terms of the number of brood cells at 14 and 21 days comparend with control grup.

The aim of this paper was to measure the main aspects that characterize the feeding behavior in six months old calves fed using three different feed spaces allowance. During the experiments the following feeding behavior aspects were determined: number of feeding periods, the length of feeding periods and the total length of feeding periods. Results showed that calves spent feeding, on average, 292 minutes in the morning, 198 minutes in the afternoon and only 71 minutes during the night when the length of feeding space was 0.6 m per calf. Calves spent feeding 175 minutes during the morning, 193 minutes during the afternoon and 53 minutes during the night when the length of the feeding space was 0.32 m per calf. Calves spent feeding 255 minutes during the morning, 227 minutes during the afternoon and 30 minutes during the night when the length of the feeding space was 0.28 m per calf. The total time spent feeding by calves was 560 minutes when the length of feeding space was 0.6 m per calf, 421 minutes when the length of feeding space was 0.32 per calf and 512 minutes when the length of the feeding space was 0.28 per calf. There were significant differences (p

Based on the bibliographic study it has been observed the fact that poleniferous plants have diverse blooming, depending on the time of the day. Temperature and humidity are climatic factors with a special influence in opening the corolla of a flower and production of pollen for bees. Also, every species has different needs for temperature degrees in order to bloom. The bees start collecting the pollen from the early hours in the morning if the temperature is over 140C, and the collection lasts until the evening. During this activity, the bees visit a large number of flowers, as the blooming of some poleniferous plant species depends very much on the moment of the day. This process is easy to track by watching the entrance of the beehive for the bees that enter loaded with pollen clusters on their legs. If observations start from 8 o’clock it can be seen that around midday the colour of the pollen clusters on bees’ legs change, so that at 12 o’clock none of the bees that were bringing yellow pollen from the dandelion in that moment brings any more pollen from them because the dandelion has closed its corolla and for the bees it no longer represents a poleniferous source at midday. After 12, fructiferous trees are an important source of pollen. The pollen has different qualitative and nutrient properties depending on the plant species nurturing the bees.

The study was carried out using a number of 14 chinchillas, 12 males and 2 females. The purpose was to determine the slaughter parameters. The main characteristics used on the study were: different organs weight and size. The mean weight of the group before slaughtering was 458.33 g and after slaughtering 352.5 g. It has been noticed that the measurements taken in the study were positively correlated.

The study was made in 2008 and his purpose was to establish the period of time necessary to execute the main services in a chinchilla farm in order to do the standardisation of work and to establish the payment It has been determined the period of time necessary for giveing the hay, the fodder, the water, checking the animal births, spreading out the sand, etc., wich was 3 hours and 25 minutes. 3 workers participated in this daily program, so wich one of them worked for 3 hours and 8 minutes every day.

the paperwork present the results of a comparative study regarding the production of acacia (Robinia pseudacacia) and lime honey harvested in 2008 in flat, vertical and multi-frame hives. A total of 45 bee families (Apis mellifica carpatica, Banatica ecotipe), divided in three experimental groups, with 15 families on each hive, were examined for every type of hive. During the experiment there were tracked the number of honeycombs with larvae starting from 7th to 10th of April and from 1st to 5th of May, the acacia and lime honey yield.

Studies were conducted on a population of Russian sturgeon (Acipenser güeldenstaedti) age between 132-175 days, raised in recirculation system. Based on body weight, the population was divided into two categories: 50-100 g and 100-150 g. On the 42 individuals were performed determinations of weight, length, width, height and circumference. It was also determined the number of shields which comprise the five lines of shields, which are endowed with Russian sturgeons. On the basis of weighing and measurements taken have resulted following body indices: the index of profile (height), the index of thickness (width spinal), the fattening index (Fulton index), quality index and the index of fleshiness. At the lot of Russian sturgeons with average body weight of 127.55 ± 3.75 g, body length is 76.27% of total body length, head length is 20.11% of the total length of the body, caudal peduncle length measured 16.39% of total body length and length of snout reaches 45.56% of head length. Index of profile (height) has highlighted that juvenile Russian sturgeon has an elongated body and a straight back.

It is well known that breeding is based on phenotypic and behavioural performance assessed at the level of each honeybee colony. By selection, the genes responsible for the desired characters have to be favoured, by evaluation and classification of all colonies involved in a breeding program. Generally, in the beekeeping practice, the most applied method of selection is the mass selection regarding the main objective- honey production. Some more elaborated programs use selection simultaneous selection on several characters. Until now, a standard method for honey bees evaluation and selection on several characters could not be generalized, every breeder establishing the selection method depending on proposed goals which could be different especially when we speak about different races and environmental conditions. Taking into account the selection objectives in Romania it was conceived a standardized methodology for the selection on several characters in Romanian condition

The research was performed within the recirculating aquaculture system for sturgeon growth, in Banat's Universsity of Agricultural Sciences and Veterinary Medicine from Timisoara, on 2851 juveniles of Siberian sturgeons (Acipenser baeri), aged between 132-175 days. Juveniles were maintained in 4 tanks with volume of 5.63 m3 , fed with granulated feed of 2 mm (44 & 22% PB and GB). Every 2 weeks the weight and body length was determinate on an effective of 30 individuals from each tank. Based on the obtained body weight, it has been established the food quantity used and bioproductive indicators. Having the weight differences between the individuals of a Siberian sturgeon population, it is needed at certain time intervals to practice assortment by body development. At the age of 175 days, Siberian sturgeons reached an average body weight between 90.13 ± 4.56 g and 197.63 ± 7.22 g and average body length between 28.73 ± 0.43 cm and 37.55 ± 0.38 cm. Individual values of minimum and maximum body weight varied between 32 g and 307 g and body length between 24.5 cm and 42 cm. For the entire population of Siberian sturgeons, there is a middle and high variability for the average body weight and a small variability for the average body length. During the 43 experimental days, the 2851 Siberian sturgeons have acquired a real weight gain of 184.66 kg, with a conversion factor of 0.84 kilograms of feed / kg growth and an index of feed conversion of 1.19 kilograms spore / kg feed consumed.

This work presents the results of the morphological measurements performed on working bees originating in 6 apiaries located in Caras-Severin and Timis Counties. The experiments were carried out during 15.08.2009-15.09.2009, on 412 bees belonging to the species Apis mellifica carpatica. During the experiment, we performed the following determinations: proboscis length, anterior wing length and width, posterior wing length and width, tibia length, tarsus length and we calculated the tarsal, cubital and radial indices. Successive to the statistical interpretation, we observed that the bees originating in the apiaries from Caras-Severin recorded bigger values in most parameters studied.

In this work, we tested the resistance of bee families to young bee diseases. The researches were carried out in two apiaries from Timişoara and Comoraste, Caras-Severin County. The biological material was consisted of 10 bee families belonging to the species Apis mellifica carpatica, distributed in two experimental variants of 5 families, with almost equal power. During this experiment, we assessed the degree of cleaning and removing of the young bees that died of freezing. Successive to the researches performed, in all the three controls we observed significant differences, from a statistical viewpoint (p

Although pikeperch is a promising species for intensive aquaculture and some experiments regarding all-female production in this species were made, there are no specifically data regarding genetical inactivation of pikeperch sperm. The aim of this study is to test two different UV lights (15 and 30 Watts) and exposure times of the diluted milt at UV irradiation for sperm genetical inactivation, in order to establish the first step in the gynogenesis protocol for pikeperch. The milt collected from 4 clinically health adult pikepearch males (3-4 years old) was diluted 1:9 with Ringer solution, after that being placed into Petri dishes (Ø = 3 cm) in a thin layer of 1 mm. Two UV lights (λ = 253.7 nm) at 15 Watts (variant V1) and 30 Watts (variant V2) were used in our experiments. These were placed 20 cm above Petri dishes, and were established for each variant, 5 different periods for exposure to the irradiation: 8 min. (T1), 11 min. (T2), 14 min. (T3), 17 min. (T4) and 20 min. (T5). The successful of irradiation was assessed by percent of fertilization (at 2 hours post fertilization), daily percent of survival, percent of hatching and percent of haploids (embryos/larvae with severely curved backbones and other deformities). After this study we could conclude that genetically inactivation of the pikeperch sperm can be performed with 15 Watts and 30 Watts UV lights at an exposure time which can vary between 8 and 20 minutes.

The studies on coypu behaviour are applied in the development of knowledge regarding ethology, in the coypu-breeding technology, animal welfare and, at the moment, they present great importance for animal protection. The biological material studied was consisted of health adult coypus, belonging to the variety Golden Standard with a similar body mass. The indices supervised were: feeding behaviour duration if coypus are fed with barley and carrots according to gender and time slot, the duration of one feeding sequence according to forage consumed and gender and the number of feeding sequences according to forage consumed and gender. For realising the purposed aim, each coypu was monitorized (video), individually, 24 hours a day. The duration of feeding behaviour when coypus are fed with barley and carrots was 4507.88±38.99 seconds for males (5.21% of the behavioural manifestations), and for coypu females, the duration of feeding behaviour was 4576.63±26.01 seconds (5.29% of the behavioural manifestations). The most intense manifestations of the feeding behaviour took place in the time slots 08:00-14:00 and 14:00-20:00, and the lowest intensity of the feeding behaviour manifestations took place in the time slot 02:00-08:00.

Knowing the behaviour of one species, with interest in animal husbandry science represent a compulsory necessity for the development and improvement of the breeding and exploiting technology for this species. The studies on coypus behaviour are applied in the development of knowledge regarding ethology, coypu breeding technology, animal welfare and, at the moment, this present great importance for animal protection. The biological material studied was consisted of adult coypus, belonging to the variety Golden Standard. The supervised indices were: feeding behaviour duration if coypus are fed with maize grain and fodder beet according to gender and time slot, the duration of one feeding sequence according to forage consumed, gender and time slot and the number of feeding sequences according to forage consumed, gender and time slot. For realising the purposed aim, each coypu was monitorized (video), individually, 24 hours a day. The duration of feeding behaviour when coypus are fed with maize grain and fodder beet was 4921.63±472.64 seconds for males (5.69% of the behaviour al manifestations), and for coypu females, the duration of feeding behaviour was 5450.00±297.94 seconds (6.30% of the behaviour al manifestations). The most intense manifestations of the feeding behaviour occurred in the time slot 08:00-14:00, and the lowest intensity of the feeding behaviour manifestations occurred in the time slot 02:00-08:00. The average total number of males feeding sequences if coypus are fed with maize grain and fodder beet was 51.63 ± 5.54 sequences and the average total number of females feeding sequences was 56.38 ± 2.48 sequences.

The purpose of this work was to assess reference values of serum biochemical indices (enzymes, metabolites and minerals) in some representative fish species belonging to Acipenseridae, Cyprinidae, Esocidae and Salmonidae family in order to establish a baseline data which will serve to monitoring nutritional-metabolic balance and healthy condition of these species from aquatic biocenosis or intensive exploitations. Serum samples were analyzed for glucose, total proteins, albumine, urea nitrogen, creatinine, uric acid, triglycerides, cholesterol, ALT, AST, GGT, alkaline phosphatase, amylase, total bilirubin, Ca, P, Mg, Fe, using a FullyVet automated chemical analyser. The obtained results were compared with those from other papers regarding serum biochemical profile of fresh water fish species. Serum biochemical reference intervals were as follows: glucose, 28.41±0.80–64.00±1.41 mgdL-1; total proteins, 2.78±0.21-4.05±0.20 gdL-1; albumine, 0.67±0.12–1.59±0.11 gdL-1; urea nitrogen, 12.16±0.78–18.30±0.27 mgdL-1; creatinine, 0.06±0.01–0.27±0.00 mgdL-1; uric acid, 1.00±0.00–1.66±0.09 mgdL-1; triglycerides, 46.00±1.29– 351.3±12.3 mgdL-1; cholesterol, 123.00±2.12–198.00±0.91±mgdL-1; ALT, 15.00±0.91–32.00±1.29 IU L-1; AST, 30.00±0.91–92.00±1.47 IU L-1; GGT, 4.00±0.00–6.33±0.46 IU L-1; alkaline phosphatase, 60.33±1.20–109.50±3.05 IU L-1; amylase, 28.08±0.93 – 36.00±0.91 IU L-1; total bilirubin, 0.00±0.00–0.03±0.00 mgdL-1; Ca, 7.63±0.40–12.36±0.50 mgdL-1; P, 11.83±0.35-30.48±0.26 mgdL-1; Mg, 1.80±0.14–3.88±0.21mgdL-1; Fe, 57.60±3.48–120.00± 1.08 mgdL-1. The wide intra- and interspecific variability of our data requires subsequent studies of the endo- and exogenous factors (living condition, season, age, gender, origin, breeding system, physiological and nutritional status, genetic of each individual, etc.) that can induce variations of the propound parameter.

The body measurements was made on 20 males of river lobsters (Astacus fluviatilis or Astacus astacus). The lobsters on witch the study was made had an average total length of body of 12.72 cm ±0.19 cm (formed from: 6.07±0.10cm the length of cefalothorax, 4.73 ±0.10 cm the length of abdomen and 1.91 ±0.07 cm length of telson), and a body weight of 55.70±3.07 g. the males present a length of feelers of 8.53±0.34 cm, a antennas of 2.20±0.05 cm, and the stem of the eyes of 0.51±0.02 cm. The body of male present a average width of 3.35±0.07 cm at level of cefalothorax, and 2.40±0.05 cm at abdomen level. Average deepness of cefalothorax was 2.85±0.07cm, and abdomen deepness of 1.34±0.03cm. The 5 pairs of periopode had an average length of 10.91±0.42 cm, 6.06±0.22 cm, 7.21±0.21 cm, 6.57±0.16 cm and 5.85±0.15cm. The average length of pleopods was at first pear of 2.00±0.06 cm, at second pair of 2.11±0.05 cm, at third of 1.85±0.05 cm, at fourth of 1.73±0.04 cm and at fifth pair of 1.55±0.04 cm.

The body measurements was made on 20 males of river lobster (Astacus fluviatilis or Astacus astacus). The lobsters on witch the study was made had an average total length of body of 12.72 cm ±0.19 cm (formed from: 6.07±0.10cm the length of cefalothorax, 4.73 ±0.10 cm the length of abdomen and 1.91 ±0.07 cm length of telson), and a body weight of 55.70±3.07 g. the males present a length of antennas of 8.53±0.34 cm, a feelers of 2.20±0.05 cm, and the stems of the eyes of 0.51±0.02 cm. The body of males present a average width of 3.35±0.07 cm at level of cefalothorax, and 2.40±0.05 cm at abdomen level. Average deepness of cefalothorax was 2.85±0.07cm, and abdomen deepness of 1.34±0.03cm. The 5 pairs of periopodes had an average length of 10.91±0.42 cm, 6.06±0.22 cm, 7.21±0.21 cm, 6.57±0.16 cm and 5.85±0.15cm. The average length of pleopods was at first pears of 2.00±0.06 cm, at second pair of 2.11±0.05 cm, at third of 1.85±0.05 cm, at fourth of 1.73±0.04 cm and at fifth pair of 1.55±0.04 cm.

Non additive genetic component that is the base of a quantitative character has no predictability, this is the reason for which the present paper is proposing to appreciate the special combinatory capacity for corporal mass of the descendants at birth in a specific crossing scheme combining three rabbit breeds common in Timis county breeders New Zealand White, Large Chinchilla and Californian. The data obtained, statistic presented prove a good special combinative capacity for this character, reason for which the crossing scheme is recommended for producing meat individuals destined for slaughterhouses. This scheme provides an exploitation of the high fervency of the heterozygote at maternal forms and at the final hybrids.

Romanian Mioritic Shepherd Dog, was selected from a natural population breed in Carpathian Mountains. The aim of this paper was to estimate variance at 12 body measurements using 26 males from Romanian Mioritic Shepherd Dog breed. The animals were registered with the Romanian Mioritic Association Club from Romania. The statistical data showed that there is a large variance for body length and tail length, a middle variance for the croup width and thorax width and a small variance for height at withers, height at middle of back, height at croup, height at the base of the tail, depth of thorax, thoracic perimeter, elbow height and height of the hock. We recommend of breeders dogs from this breed to take account in genetic improvement programs, of values presented in this paper.

This work presents the results of hibernation of bee colonies maintained in multi-storied beehives endowed with anti-varroa ground, made of wood and polystyrene Dadant. The experiments were carried out Banat’s University of Agricultural Sciences and Veterinary Medicine „King Michael the Ist” from Timişoara, Romania, between the 1st of November 2016 and 1st of March 2017. The biological material was represented by 20 Apis mellifera carpatica bee colonies, divided in two experimental variants, 10 colonies/batch, with similar power and same-age queens. During the experiment, we observed the bee amount at the start of hibernation; the bee amount at the end of hibernation; the evolution of feed intake and losses caused by mortality. At the end of the experiment, we determined a bigger bee amount in the polystyrene Dadant beehives, significantly lower losses caused by mortality from a statistical point of view (p

The aim of this paper was to analyze, within a group of 26 males from Romanian Mioritic Shepherd Dog breed, 13 characters of genetically improved, characters stipulated in, „Selection sheet and body measurements for Romanian shepherds". The animals were registered with the Romanian Mioritic Association Club from Romania. Romanian Mioritic Shepherd Dog, was selected from a natural population breed in Carpathian Mountains. In order to develop a genetic improvement program at this effective of 26 males from Romanian Sheperd Dog breed, found in evidence of Romanian Mioritic Association Club from Romania, should be considered the following conclusions on variance those 13 characters studied in this paper, respectively, the variability was middle for the width of skull and ear and low for the other 11 characters analyzed. Also, this paper highlighted the following reports of the characters analyzed at the males taken in the study: the ratio between the average of length and width skull was 1.005:1, the ratio between the average of length skull and the average of length muzzle was 1.31:1 and between average of the width of skull and the muzzle was 1.82:1. By Comparing between them length, width and depth of muzzle, resulted a ratio of 1.38:1:1.10.

The plasma testosterone determinations were performed on 19 silver fox males (Vulpes fulva) and 19 polar fox males (Alopex lagopus) that were coming from the farm. The concentration of testosterone was performed on prepubertal males (0-10 months), during the sexual period and the period of sexual rest. For quantitative determination of the testosterone, some systems were used like the VITROS system ECi/ECi O2, the immunological diagnostic system VITROS 3600 and the integrated system VITROS 5600. In both species, the plasma concentration was maximal at the males in the period of sexual activity (1.56±0.23 ng/ml at the polar fox and 1.46±0.23ng/ml at the silver fox) and minimal in males between the age 0-10 months (0.22±0.01 ng / ml at the polar foxes and 0.20±0.01 ng/ml at the silver foxes).The adult males that were in the sexual rest period showed low testosterone levels (0.25±0.02 ng/ml at the polar foxes and 0.22±0.02 ng/ml at the silver foxes).

Essential oils obtained from medicinal and aromatic plants, due to their bioactive principles, represent an important pharmaceutical source for human and animals. The objective of this paperwork was to determine the effect of the addition in supplementary feeding of bee colonies of oregano and basil essential oils. The bee colonies fed, during spring, on sugar syrup and essential oils presented a better development, recording a number of bee brood cells that was bigger than in the control variant. At the same time, we observed a significant reduction in the total number of germs within the small intestine in the case of supplementation with these essential oils, correlated with the improvement of health status of bee colonies.

The aim of this paper was to analyze, within a group of 21 females from Romanian Mioritic Shepherd Dog breed, 13 characters of genetically improved, characters stipulated in „Selection sheet and body measurements for Romanian shepherds". The animals were registered with the Romanian Mioritic Association Club from Romania. Romanian Mioritic Shepherd Dog, was selected from a natural population breed in Carpathian Mountains. Following the analysis for the mean value and dispersion indices for those 13 body measurement was realized a better knowledge of the genetic structure of the population for these characters and was created the possibility of estimating sexual dimorphism. Also, this paper highlighted the following reports of the characters analyzed at the males taken in the study: the ratio between the average of length and width skull was 1.00:1.02 the ratio between the average of length skull and the average of length muzzle was 1.36:1.00 and between average of the width of skull and the muzzle was 1.75:1.00. By Comparing between them length, width and depth of muzzle, resulted a ratio of 1.25:1.00:1.14. Also, this paper highlights the fact that the variability was medium for head length, skull length and skull width.

The aim of this paper was to measure the main aspects that characterize the feeding and rumination behavior of three months old calves during the summer season when daily temperatures were normal (daily peak of +30°C) and during days with very high temperatures (daily peak +41°C). During the experiments the following feeding and rumination behavior aspects were determined: number of feeding periods, the length of feeding periods, number of ruminating periods and length of rumination periods. Results showed that calves spent feeding, on average 383.7 minutes per day during 13.1 feeding periods with an average length of 29.5 minutes when the when the daily temperatures were normal and 341.7 minutes per day during 12.9 feeding periods with an average length of 26.7 minutes when the daily temperatures were very high. Calves ruminated on average 396.1 minutes per day during 15.6 rumination periods with an average length of 25.5 minutes during the summer season when temperatures were normal (daily peak of +30°C). Calves ruminated on average 331.3 minutes per day during 13.1 rumination periods with an average length of 25.5 minutes during the summer season when temperatures were very high (daily peak of +41°C).

This paper presents the effects of acidifying substances (lactic acid or acetic acid), Enterobiotics products (Lactobacillus acidophilus LA-14 and Bifidobacterium lactis BI-04) and Enterolactis Plus (Lactobacillus casei) on the wax glands of worker bees. The research was conducted in Timis County, Romania, between March 25 and April 20, 2011, on 110 colonies of bees (Apis mellifera carpatica), allocated to 11 experimental treatment groups. Colonies in the experimental groups were given three weekly feeds of sugar syrup supplemented with acidifying substances (lactic acid or cider vinegar) and/or probiotic products (Enterobiotics or Enterolactis Plus). Three weeks after the administration of the experimental diets, 10 worker bees from each treatment group were sampled for histological examination of their wax glands. Gland development was shown to be influenced by administration of prebiotic and/or probiotic supplements. Wax gland cell sizes ranged from 25.1 microns for the control group to between 27.8 and 31.8 microns in the group fed with acidifying substances and between 26.9 and 29.2 microns in bees fed with probiotic products. Bees supplemented with both lactic acid and probiotic product (group LE9 and LE10) showed mean wax cell sizes of 31.8 microns.

Romanian Mioritic Shepherd Dog, was selected from a natural population breed in Carpathian Mountains. The aim of this paper was to estimate variance at 12 body measurements using 23 females from Romanian Mioritic Shepherd Dog breed. The animals were registered with the Romanian Mioritic Association Club from Romania. In order to develop a genetic improvement program at this effective of 23 females from Romanian Sheperd Dog breed, found in evidence of Romanian Mioritic Association Club from Romania, should be considered the following conclusions on variance those 12 characters studied in this paper, respectively, there is a large variance for the height at the middle back, the height at the croup, the height at the base of the tail, the width of the croup, the length of the tail, the depth of the thorax, the thorax perimeter, the height of the elbow and for the height at the withers, the body length and the height at the hocks, the variance is middle.

To ease the shock of weaning, the young hybrid rabbits were held between the ages of 30 days and 37 days in a farrowing cage and than they were transferred inside growing pens (148/123 cm), where they remained until slaughter. Before slaughter, youth has undergone a finishing process. Youth received treatment with vitamins, coccidiostatics and specific vaccinations. The finishing process began when youth Hybrid reached a body mass of 1700-1900 g and consisted of removal of the ration of grain and vitamins. Youth hybrid was slaughtered to a body weight of 2500 g, weight being achieved between the ages of 107-142 days. Youth consumed between 140.86 to 663.50 g / day juicy fodder, from 806.14 to 1225.82 g / day bulky and concentrated feed. Carcasses of youth hybrid weighed between 1596.70 to 1886.40 g. Slaughter yield ranged from 50.25 to 68.67%. The average price on carcass in young hybrid rabbits, calculated at a price of 17 lei/kg carcass, ranged from 27.10 to 33.40 lei / carcass.

In order to carry out the experiment, we made 5 hybrid rabbit groups (each one consisted of 5 individuals), homogenous in terms of age and body development, bred in cages of wire gauze. The control group was offered simple drinking water, and the other groups were offered drinking water supplemented with extracts of horseradish, dog-rose, French tamarisk and grape seeds. In each cage, we placed a stainless steel feeder for the administration of granulated forage and a plastic water supplier (300 ml capacity). During the experiment, we determined the granulated forage intake (150 g/individual/day) and the weekly body mass (between 35-105 days). At the end of the experiment, the biggest mean body mass was observed in the young rabbits that used to drink water with horseradish extract (3172±377 g), followed by those with French tamarisk (3046±162 g), dog-rose (2949±366 g) and finally by the rabbits that drank grape extract (2888±250 g). Along the 10 experimental weeks, the control group recorded significant differences (p

At the end of the experimental period (105 days), the entire group of young rabbits studied was slaughtered. Successive to this, we determined the following indices: mean live body weight at the end of the experimental period, mean carcass weight, mean gain at slaughtering, mean mass of internal edible organs (liver, heart, lungs and kidneys) and the proportion of internal organs in carcass mass, in hybrid young rabbit. According to the analyses of differences between the control rabbit group and the group with French tamarisk, the group with horseradish and the one with grapes, we observed significant differences (p0.05). By comparing the experimental groups to each other, we determined insignificant differences (p>0.05) between all the parameters studied at slaughtering.

In the experiment consisted in using 5 females hybrid which have been mated with 3 male pure-bred. Each couple was monitored for pup and mother body weight and also the consumption of succulent feed, bulky and concentrated. Both parents and offspring were kept in cages made of wood and wire mesh. Under the cages there was a tray of galvanized steel sheet for collecting manure. During the experiment, female New Zealand and the Californian Red hybrid have given birth 4 times, producing 43 cubs (cubs 10.75/birth) and 31 cubs (cubs 7.75/birth). The other 3 females gave birth only once a year. All pups survived until weaning (30 days). Weaned cubs from female New Zealand Red hybrid at time of weaning had a mean body mass of between 341.20 to 602.20 g/cubs, and those from female Californian hybrid were between 374.00 to 803.33 g/cub. At weaning, cubs from female Chinchilla Mare hybrid weighed on average 546.75 g/cub, the ones from the French hybrid female Great Silver weighed 433.00 g/cub, and those from the New Zealand Red primiparous female hybrid weighed784.00 g/weaned cubs. Between the 11th and the 30th day of lactation, most females have lost body weight, the highest being 489.00 g/lactating female.

The biological potential of a bee family is mainly generated by the biological value of the queen. Whether we grow queens widely or just for our own apiaries, we must consider the acquisition of high-quality biological material, and also the creation of optimal feeding and caring conditions, in order to obtain high genetic value queens. Queen breeding technology starts with the setting of hoeing families, nurse families, drone-breeding families – necessary for the pairing of young queens, and also of the families which will provide the bees used to populate the nuclei where the next queens will hatch. The complex of requirements for the breeding of good, high-production queens is sometimes hard to met, under the application of artificial methods. The selection of breeding method must rely on all these requirements and on the beekeeper’s level of training.

Agricultural wastes represent a large unexploited energy potential that could be converted into biogas by anaerobic digestion. In the present study there has been analysed the way in which agricultural wastes are converted into biogas by using as inoculum cattle manure as compared with activated sludge. To carry out this experiment on small scale there have been designed 5 batch bottles. For the batch process all substrate was put into the bottles at start. The biogas process was initiated after closing the bottles, and the biogas was collected during the process until the biogas production ended. During the 33 days of experiment the batch bottles were held at constant temperature (370C) in a water bath. The biomass used in the experiment was wheat straw and corn stalks. Before being used in the experiment the biomass was milled using a kitchen mixer. After that the biomass was subjected to a combination of thermal and chemical pretreatments. The volume of biogas produces was measured every seven days during the experiment period. The biogas production measurements were done by using a BlueSens measuring equipment.

No detailed histological study of the segments of the digestive tract and of the post-diaphragmatic annex glands of Chinchilla laniger is available in the literature, to our knowledge. The study presented draws attention to the morphological characteristics of the digestive tract and their involvement in the digestive process, with important implications for the composition of formula diets. Histological study of the digestive tract and annex glands (liver and pancreas) of Chinchilla laniger shows no major differences from other mammals. The walls of the oesophagus, stomach and intestine are composed of four layers: the mucosa, sub-mucosa, muscularis mucosa and serosa (the fourth layer of the oesophagus being called the adventitia). A noteworthy feature of the species is the generous development of the caecum in proportion to body size, a characteristic shared with other rodent species.

The study was conducted within the recirculating aquaculture system for sturgeon rearing in Herneacova village, Timis County, on a population of juvenile sterlet (Acipenser ruthenus) aged between 346-431 days. Specimens were housed in five round tanks (Ø=3 m, V=6 m3, h=100 cm) and fed with commercial extruded pellets (3 mm granulation, 48% CP, and 10% EE). Fish from each tank were individually weighed and measured once every two weeks. Bioproductive indices such body weight gain (BW), specific growth rate (SGR), and feed conversion ratio (FCR) were calculated at the end of an 85 days experimental period. Statistical analysis was performed using the SPPS IBM 22 and Minitab 16 software. Data were reported as Mean±Sx at a significant level of p

The study of the main body mass indices in the Giant German Lop breed was performed between 6.06.2014 and 10.06.2014 in a rabbitry from Diniaș town, Timiș County. The biological material was represented by 16 adult rabbit females and 10 adult males, with the average body weight of 7 kg, raised in semi-intensive system. There have been performed the following body measurements: oblique and horizontal length of the trunk, the length of thorax, the length of head, the length of ears, of neck, and of tail, the height at withers and rump, the height of thorax and stern, the width of chest, of thorax, of rump at hip and ischia, the wide width of forehead, the thoracic circumference and the circumference of shin. Giant German Lop males have registered higher values in the body size index, in the pelvic-thoracic index, and in the rump angle index compared to females from the same breed, which have registered higher values in the index of transverse body size, in the index of massiveness, in the pelvic-thoracic index, and in the cephalic index.

Our research was performed on juvenile sterlets (Acipenser ruthenus), aged between 346-431 days, maintained in five round pools (Ø=3 m, V=6 m3, h-100 cm) and fed with a commercial extruded pellet (3 mm granulation, 48% CP, and 10% EE). Body weight and total body length on 30 individuals from each tank were determined every two weeks. Weighing and somatic measurements achieved at the end of experimental period (standard length-l, body weight-G, maximum body high–H, maximum body perimeter (Pmax), head length–lh,, caudal peduncle length-lp) allowed us to calculate the following body indices: profile index, thickness index, Fulton index, Kiselev index, meat index. Statistical analysis was performed by using the SPSS IBM 22 and Minitab 16 software. Data were reported as Mean±Sx at a significant level of p

The histological studies of the male genital apparatus of the Chinchilla Laniger are scares and incomplete. Due to the studies done on the male genital apparatus of Chinchilla Laniger it has been determined that the seminiferous tubes are separated by large conjunctive septum; the seminiferous epithelium varies from one tissue to another, aspect that suggests different stages in the spermatogenesis process. The epididymis channel is made up of pseudo-layered epithelium, a reduced lamina propria and a delicate muscular layer. The deferent channel wall is made up of three tunics: mucous, muscular and adventitia. The muscular tunic is the most developed part and is made up of three super posed layers of even smooth muscle cell. The histological sections done on the pelvic urethra reveals the following components: mucous, vascular layer, muscular and adventitia. The transversal sections done on the free extremity of the penis reveal the presence of the foreskin made up of three layers: tegument or external, parietal or visceral. The vesicular glands (seminal) have an intestine shaped aspect and are sustained by their own ligament, richly vascularised. The transversal section reveals a tubular aspect, the wall of these tubes is made up of: mucous, muscular and adventitia. The prostate is made up of an external part and an internal part, and is covered in a thick conjunctive-muscular capsule.