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Fungal strains capable of degrading oxytetracycline, norfloxacin and sulfadiazine were screened from the soil contaminated by heavy metals. The strains were enriched and domesticated in medium with the antibiotics as the sole carbon source. After isolation and purification of antibiotic-resistant fungi, and the purified strains were returned to the liquid medium with antibiotics as the sole carbon source. Then used high-performance chromatography(HPLC) and UV spectrophotometry to detect the antibiotic degradation ability of each strains, and those strains were identified by morphological characteristics, the ITS DNA sequence determination and phylogenetic analysis. Four strains of antibiotic-degrading fungi named KS248, KS256, KS257, and KS272 were screened and identified as Fusarium verticillioides, Fusarium solani, Aspergillus sydowii, and Penicillium janthinellum. Among them, strains KS248, KS256, and KS257 had the ability to degrade oxytetracycline, norfloxacin, and sulfamethazine; strain KS272 had the ability to degrade oxytetracycline and norfloxacin. Under antibiotic content of each antibiotic was 1500 μg·L-1, 30℃, 150 r·min-1, after 7 days dark culture, the strain KS272 had the strongest ability to degrade oxytetracycline and norfloxacin with the degradation rates of 40.29% and 10.49% respectively; The strain KS256 had the strongest ability to degrade sulfamethazine and the degradation rate was 18.53%. The strains had the ability to degrade two or more antibiotics. The ability of strains to degrade antibiotics from the highest to the lowest was oxytetracycline, norfloxacin, and sulfamethazine. As the concentration of antibiotics increased, the ability of the strains to degrade each antibiotic was weakened.