1. Establishment of a FDC-P1 murine cell line with human KIT N822K gene overexpression
- Author
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O. G. Leonova, Elmira Vagapova, Vladimir S. Prassolov, T D Lebedev, Pavel Spirin, and Vladimir I. Popenko
- Subjects
Stromal cell ,Biochemistry ,Receptor tyrosine kinase ,receptor tyrosine kinase KIT ,KIT N822K ,03 medical and health sciences ,0302 clinical medicine ,acute myeloid leukemia (AML) ,hemic and lymphatic diseases ,FDC-P1 ,medicine ,Molecular Biology ,stromal cells ,Acute leukemia ,biology ,Myeloid leukemia ,medicine.disease ,Leukemia ,Haematopoiesis ,Cell culture ,030220 oncology & carcinogenesis ,biology.protein ,Cancer research ,Molecular Medicine ,Ectopic expression ,Research Article ,030215 immunology ,Biotechnology - Abstract
The mechanism of resistance of leukemia cells to chemotherapeutic drugs remains poorly understood. New model systems for studying the processes of malignant transformation of hematopoietic cells are needed. Based on cytokine-dependent murine acute myeloid leukemia (AML) FDC-P1 cells, we generated a new cell line with ectopic expression of the KIT gene encoding mutant human receptor tyrosine kinase (N822K). We investigated the role played by overexpression of the mutant KIT in the survival of leukemia cells and their sensitivity to therapeutic drugs. We also generated a co-culture system consisting of FDC-P1 murine leukemia cells and a HS-5 human stromal cell line. Our data can be used for a further comprehensive analysis of the role of KIT N822K mutation in the cellular response to anti-leukemic drugs, growth factors, and cytokines. These data are of interest in the development of new effective therapeutic approaches to the treatment of acute leukemia.
- Published
- 2020