1. Conformational dynamics of a single protein monitored for 24 h at video rate
- Author
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Ye,Weixiang, Götz, Markus, Celiksoy,Sirin, Tüting, Laura, Ratzke,Christoph, Prasad,Janak, Ricken,Julia, Wegner,Seraphine V., Ahijado Guzmán, Rubén, Hugel,Thorsten, Sönnichsen, Carsten, Ye,Weixiang, Götz, Markus, Celiksoy,Sirin, Tüting, Laura, Ratzke,Christoph, Prasad,Janak, Ricken,Julia, Wegner,Seraphine V., Ahijado Guzmán, Rubén, Hugel,Thorsten, and Sönnichsen, Carsten
- Abstract
We use plasmon rulers to follow the conformational dynamics of a single protein for up to 24 h at a video rate. The plasmon ruler consists of two gold nanospheres connected by a single protein linker. In our experiment, we follow the dynamics of the molecular chaperone heat shock protein 90 (Hsp90), which is known to show “open” and “closed” conformations. Our measurements confirm the previously known conformational dynamics with transition times in the second to minute time scale and reveals new dynamics on the time scale of minutes to hours. Plasmon rulers thus extend the observation bandwidth 3–4 orders of magnitude with respect to single-molecule fluorescence resonance energy transfer and enable the study of molecular dynamics with unprecedented precision., Depto. de Química Física, Fac. de Ciencias Químicas, TRUE, pub
- Published
- 2024