1. The SARS-COV-2 spike protein binds sialic acids and enables rapid detection in a lateral flow point of care diagnostic device
- Author
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Phillip J. Stansfeld, Muhammad Hasan, Robert A. Field, Thomas R. Congdon, Collette S. Guy, Alexander James Zwetsloot, Simone Dedola, Sarah-Jane Richards, Marc Walker, Simona Chessa, Alexander N. Baker, Józef R. Lewandowski, Angelo Gallo, Giulia Pergolizzi, Anne Straube, and Matthew I. Gibson
- Subjects
Coronavirus disease 2019 (COVID-19) ,General Chemical Engineering ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,viruses ,010402 general chemistry ,medicine.disease_cause ,01 natural sciences ,chemistry.chemical_compound ,RA0421 ,Neuraminic acid ,medicine ,QD1-999 ,Coronavirus ,Point of care ,chemistry.chemical_classification ,010405 organic chemistry ,Spike Protein ,virus diseases ,General Chemistry ,3. Good health ,0104 chemical sciences ,Chemistry ,chemistry ,Biophysics ,Spike (software development) ,Glycoprotein ,Research Article ,RC - Abstract
There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform., The spike-protein from SARS-COV-2 is shown to bind sialic acids, which is exploited to assemble a lateral flow diagnostic tool, using glycans rather than antibodies, as the recognition unit.
- Published
- 2020