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The effect of mix-cultured aerobic denitrifying microorganisms on the water remediation has been extensively explored, but little is known about the performance of mix-cultured low efficiency fungi on denitrification. In this study, two kinds of aerobic denitrifying fungi (Trichoderma afroharzianum H1 and Aspergillus niger C1) were isolated from reservoirs, improved the capacity by mix-cultured. The results showed a difference between northern and southern reservoirs, the dominants of genera were Cystobasidium and Acremonium. The removals of total nitrogen (TN) was 12.00%, 7.53% and 69.33% in Trichoderma afroharzianum H1, Aspergillus niger C1 and mix-cultured (C1 and H1) under the denitrification medium. The contents of ATP and electron transport system activity in mix-cultured amendment were increased by 42.54% and 67.52%, 1.72 and 2.91 times, respectively. Besides, the raw water experiment indicated that TN removals were 24.05%, 12.66% and 73.42% in Trichoderma afroharzianum H1, Aspergillus niger C1 and mix-cultured. The removals of dissolved organic carbon in mix-cultured were increased 35.02% and 50.46% compared to Trichoderma afroharzianum H1 and Aspergillus niger C1. Therefore, mix-cultured of two low efficiency aerobic denitrifying fungi has been considered as a novelty perspective for mitigation of drinking water source. • Trichoderma afroharzianum H1 and Aspergillus niger C1 were isolated from reservoirs. • Mix-cultured of two fungi improved the performance of aerobic denitrifying. • Mix-cultured could remove 69% total nitrogen and 93% of dissolved organic carbon. • The ATP and electron transport system activity were increased by mix-cultured. • Mix-cultured exhibited excellent nitrogen and carbon removal ability in raw water. [ABSTRACT FROM AUTHOR]

[Display omitted] • A SnO 2 QDs-MXene composite was synthesized for electrochemical detection of luteolin. • The expanded d-spacing of MXene providing abundant active sites for detection. • The SnO 2 QDs-MXene/GCE electrode showed an ultra-low LOD of 0.14 nM. • The SnO 2 QDs-MXene/GCE electrode achieve accurate detection in actual samples. Although there have been advancements in electrochemical catalysts for luteolin detection, their practical use is constrained by low sensitivity, inadequate selectivity, and unsatisfactory limit of detection. MXene, a class of 2D materials, possesses exceptional physical–chemical properties that make it highly suitable for electrochemical detection. Nevertheless, the self-stacking and limited interlayer spacing of MXene impede its extensive application in electrochemical detection. Herein, a SnO 2 QDs-MXene composite is synthesized for selective electrochemical detection of luteolin. Inserting SnO 2 QDs between tightly stacked MXene layers expands the d-spacing of MXene, enhancing the specific surface area and enabling abundant active sites for redox reactions. The inclusion of MXene in the modified SnO 2 QDs-MXene/GCE electrode significantly enhances electron transfer. As a result, the electrode demonstrates exceptional luteolin detection capabilities, including a wide linear range (0.1–1200 nM), high sensitivity (12.4 μA μM−1), and an ultra-low limit of detection (0.14 nM). Additionally, the SnO 2 QDs-MXene/GCE electrode exhibits good repeatability, excellent reproducibility, remarkable stability, and high selectivity, making it suitable for practical sample analysis. This research contributes to advancing ultra-low limit of detection sensors for accurate luteolin detection. [ABSTRACT FROM AUTHOR]