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Recent updates on Magnetic Nano-Particles (MNPs) based separation of nucleic acids have received more attention due to their easy manipulation, simplicity, ease of automation and cost-effectiveness. It has been indicated that DNA molecules absorb on solid surfaces via hydrogen-bonding, and hydrophobic and electrostatic interactions. These properties highly depend on the surface condition of the solid support. Therefore, surface modification of MNPs may enhance their functionality and specification. In the present study, we functionalized Fe 3 O 4 nano-particle surface utilizing SiO 2 and TiO 2 layer as Fe 3 O 4 /SiO 2 and Fe 3 O 4 /SiO 2 /TiO 2 and then compare their functionality in the adsorption of plasmid DNA molecules with the naked Fe 3 O 4 nano-particles. The result obtained showed that the purity and amount of DNA extracted by Fe 3 O 4 coated by SiO 2 or SiO 2 /TiO 2 were higher than the naked Fe 3 O 4 nano-particles. Furthermore, we obtained pH 8 and 1.5 M NaCl as an optimal condition for desorption of DNA from MNPs. The result further showed that, 0.2 mg nano-particle and 10 min at 55 °C are the optimal conditions for DNA desorption from nano-particles. In conclusion, we recommended Fe 3 O 4 /SiO 2 /TiO 2 as a new MNP for separation of DNA molecules from biological sources. [ABSTRACT FROM AUTHOR]