1. Rabies virus phosphoprotein interacts with ribosomal protein L9 and affects rabies virus replication.
- Author
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Li, Youwen, Dong, Wanyu, Shi, Yuejun, Deng, Feng, Chen, Xi, Wan, Chunyun, Zhou, Ming, Zhao, Ling, Fu, Zhen F., and Peng, Guiqing
- Subjects
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RABIES in animals , *PHOSPHOPROTEINS , *PROTEIN-protein interactions , *RIBOSOMAL proteins , *VIRAL replication , *WARM-blooded animals - Abstract
Rabies virus is a highly neurotropic virus that can cause fatal infection of the central nervous system in warm-blooded animals. The RABV phosphoprotein (P), an essential cofactor of the virus RNA-dependent RNA polymerase, is required for virus replication. In this study, the ribosomal protein L9, which has functions in protein translation, is identified as P-interacting cellular factor using phage display analysis. Direct binding between the L9 and P was confirmed by protein pull-down and co-immunoprecipitation analyses. It was further demonstrated that L9 translocates from the nucleus to the cytoplasm, where it colocalizes with P in cells infected with RABV or transfected with P gene. RABV replication was reduced with L9 overexpression and enhanced with L9 knockdown. Thus, we propose that during RABV infection, P binds to L9 that translocates from the nucleus to the cytoplasm, inhibiting the initial stage of RABV transcription. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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