1. Evaluation of Neospora caninum truncated dense granule protein 2 for serodiagnosis by enzyme-linked immunosorbent assay in dogs.
- Author
-
Jin, Chunmei, Yu, Longzheng, Wang, Yinan, Hu, Shiyue, and Zhang, Shoufa
- Subjects
- *
SERODIAGNOSIS , *NEOSPORA caninum , *ENZYME-linked immunosorbent assay , *LABORATORY dogs , *TOXOPLASMA gondii , *LABORATORY mice - Abstract
Neosporosis is an infectious disease caused by Neospora caninum , and it primarily affects cattle and dogs. An infection by N. caninum causes fetal abortion and neonatal mortality. Previous proteomics and immunoscreening analyses revealed that N. caninum dense granule antigen 2 (NcGRA2) has potential for serodiagnosis of N. caninum. Consequently, we expressed the truncated NcGRA2 (NcGRA2t), which lacks a signal peptide. We compared the serodiagnostic performances of recombinant NcGRA2t with that of truncated surface antigen 1 of N. caninum (NcSAG1t). Specificity testing using sera from mice infected with Toxoplasma gondii indicated that the NcGRA2t recombinant protein does not cross-react with T. gondii . In addition, we detected anti-NcGRA2t antibody at the acute stage in experimentally infected dogs, while detecting anti-NcSAG1t antibody during both the acute and chronic stages. Our results suggest that the levels of anti-NcGRA2 antibody reflect parasite activation in dogs. In conclusion, antibodies against NcGRA2t and NcSAG1t are suitable indicators to distinguish the acute and chronic stages of N. caninum infection. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF