Search

Your search keyword '"Hisamoto Y"' showing total 1 results
Start Over Author "Hisamoto Y" Publisher academic press inc.
1 results on '"Hisamoto Y"'

1. Quantitative real-time PCR (qPCR) – Based tool for detection and quantification of Cordyceps militaris in soil.

Author

Saragih, Syaiful Amri, Takemoto, S., Hisamoto, Y., Fujii, M., Sato, H., and Kamata, N.

Subjects
*POLYMERASE chain reaction, *CORDYCEPS, *SOIL microbiology, *DNA primers, *ENTOMOPATHOGENIC fungi, *GENE amplification
Abstract

A quantitative real-time PCR using a primer pair CM2946F/CM3160R was developed for specific detection and quantification of Cordyceps militaris from soil. Standard curves were obtained for genomic DNA and DNA extracts from autoclaved soil with a certain dose of C. militaris suspension. C. militaris was detected from two forest soil samples out of ten that were collected when fruit bodies of C. militaris were found. This method seemed effective in detection of C. militaris in the soil and useful for rapid and reliable quantification of C. militaris in different ecosystems. [ABSTRACT FROM AUTHOR]

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results