Search

Your search keyword '"Hadi, Timin"' showing total 1 results
Start Over Author "Hadi, Timin" Publisher academic press inc.
1 results on '"Hadi, Timin"'

1. Kinetic and mechanistic characterization of the glyceraldehyde 3-phosphate dehydrogenase from Mycobacterium tuberculosis.

Author

Wolfson-Stofko, Brett, Hadi, Timin, and Blanchard, John S.

Subjects
*GLYCERALDEHYDEPHOSPHATE dehydrogenase, *MYCOBACTERIUM tuberculosis, *CHEMICAL kinetics, *NUCLEOTIDE exchange factors, *CYSTEINE, *HISTIDINE, *MUTAGENESIS, *KINETIC isotope effects
Abstract

Highlights: [•] The GAPDH from Mycobacterium tuberculosis is stabilized by NAD+, allowing for its purification for the first time. [•] The kinetic mechanism proceeds through an unusual nucleotide exchange reaction. [•] The catalytic cysteine and histidine residues have been identified using thiol alkylation studies and site-directed mutagenesis. [•] The chemical mechanism has been determined using a combination of primary, solvent and multiple kinetic isotope effects. [Copyright &y& Elsevier]

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results