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For at least 9000 years dogs have been pulling sleds across the Arctic, facilitating subsistence strategies and migrations. Despite the enduring presence of dogs in the Arctic there is an absence of comprehensive studies of the material culture associate with dog sledding, including the diverse technical elements needed for the activity. This study proposes a framework for the recognition of reliable archaeological indicators of dog sledding. The outcome is based on comparisons between ethnographic information of the dog traction technology and archaeological sites from the Arctic regions of Siberia, Alaska, Canada, and Greenland using multivariate analysis. These sites were selected as case studies to encompass the breadth of geographical and Inuit cultural diversity where dog sledding traditionally has been practiced. We argue, that by using this framework it is possible to study dog sledding in the Arctic prior to the Thule Inuit period and gain more knowledge about the origin of the practice. By combining sources from ethnography, history and archaeology, our framework identified items involved in dog sledding that were universal to the practice as well as items that were regionally specific. However, the most reliable evidence for dog sledding is the presence of both sled parts, dog bones and equipment for harnessing the dogs. [Display omitted] • Ethnographic framework reveals reliable indicators for identifying dog sledding on archaeological sites through material evidence. • Determining universal and regionally specific indicators of dog sledding on prehistoric archaeological sites. • Providing a method for reexamining early archaeological sites to identify previously overlooked evidence for dog traction. [ABSTRACT FROM AUTHOR]

Abstract: The majority of ancient DNA studies on human specimens have utilised teeth and bone as a source of genetic material. In this study the levels of endogenous contamination (i.e. present within the sample prior to sampling for the DNA analysis) are assessed within human bone and teeth specimens sampled from the cemetery of Santa Lucia alle Malve, Matera, Italy. This site is of exceptional interest, because the samples have been assayed for 18 measures of biochemical and physical preservation, and it is the only one identified in a study of more than 107 animal and 154 human bones from 43 sites across Europe, where a significant number of human bones was well preserved. The findings demonstrate several important issues: (a) although teeth are more resilient to contamination than bone, both are readily contaminated (presumably through handling or washing), and (b) once contaminated in this way, both are difficult (if not impossible) to decontaminate. Furthermore, although assessed on bone samples, several of the specific biochemical and physical characteristics that describe overall sample preservation, levels of microbial attack and related increases in sample porosity directly correlate with the presence of observable contamination in both bone and teeth samples from individual samples. While we can only speculate on the cause of this relationship, we posit that they provide useful guides for the assessment of whether samples are likely to be contaminated or not. [Copyright &y& Elsevier]

• Genetic and stable isotope analyses of fur clothing from the Arctic. • Excellent preservation of endogenous mitochondrial DNA. • Genetic and macroscopic species identifications were inconsistent in a third of the sampled garments. • Stable isotope analysis revealed diversity of dog diets across the Arctic. Among Indigenous populations of the Arctic, domestic dogs (Canislupus familiaris) were social actors aiding in traction and subsistence activities. Less commonly, dogs fulfilled a fur-bearing role in both the North American and Siberian Arctic. Examples of garments featuring dog skins were collected during the 19th-20th centuries and are now curated by the National Museum of Denmark. We sequenced the mitochondrial genomes of macroscopically identified dog skin garments. We conducted stable carbon and nitrogen isotope ratio analysis of the dog furs and of fur samples from contemporaneous pelts of Arctic (C. lupus arctos) and grey (C. lupus) wolves. Despite the presence of biocides used to protect the fur clothing during storage, we extracted well-preserved DNA using a minimally-invasive sampling protocol. Unexpectedly, the mtDNA genomes of one-third of the samples were consistent with wild taxa, rather than domestic dogs. The strong marine component in the diets of North American dogs distinguished them from Greenland and Canadian wolves, but Siberian dogs consumed diets that were isotopically similar to wild species. We found that dog provisioning practices were variable across the Siberian and North American Arctic, but in all cases, involved considerable human labor. [ABSTRACT FROM AUTHOR]