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As the aging population increases, the focus on elderly patients with acute respiratory distress syndrome (ARDS) is also increasing. In this article, we found progranulin (PGRN) differential expression in ARDS patients and healthy controls, even in young and old ARDS patients. Its expression strongly correlates with several cytokines in both young and elderly ARDS patients. PGRN has comparable therapeutic effects in young and elderly mice with lipopolysaccharide-induced acute lung injury, manifesting as lung injury, apoptosis, inflammation, and regulatory T cells (Tregs) differentiation. Considering that Tregs differentiation relies on metabolic reprogramming, we discovered that Tregs differentiation was mediated by mitochondrial function, especially in the aged population. Furthermore, we demonstrated that PGRN alleviated the mitochondrial damage during Tregs differentiation through the AMPK/PGC-1α pathway in T cells. Collectively, PGRN may regulate mitochondria function to promote Tregs differentiation through the AMPK/PGC-1α pathway to improve ARDS. [Display omitted] • Differential expression of PGRN and mitochondrial respiratory in ARDS patients and healthy controls. • PGRN has an equivalent therapeutic effect on Tregs differentiation in young and old ALI mice induced by LPS intratracheal instillation. • PGRN has a better promotion effect on metabolic reprogramming during the differentiation of old Tregs than that of young. • PGRN promotes mitochondrial function through the AMPK/PGC-1α pathway in T cells. [ABSTRACT FROM AUTHOR]

Tripartite motif (TRIM) family proteins play important regulatory roles in innate immune responses, the dysregulation of which cause several infectious diseases. However, the role and function of TRIM family proteins during tuberculosis (TB) infection remains unclear. In this study, we employed real-time quantitative PCR to profile the transcript levels of 72 TRIM genes from a cohort of 5 active TB patients, 5 latent tuberculosis infection (LTBI) subjects, and 5 healthy controls (HCs) in an initial discovery phase. The notable TRIM genes were assessed by in vitro cell infection experiments and further validated in another independent cohort (36 active TB, 24 LTBI and 28 HCs). The receiver operating characteristic (ROC) was used to analyze the diagnostic power of these TRIM genes. Our results revealed that 20 TRIM genes were decreased in active TB compared to LTBI and HCs. In addition, TRIM4, 16, 27, 32, 35, 46, 47, 65 and 68 were further shown to be downregulated in Mycobacterium smegmatis -infected macrophages and were found to be closely correlated with infection time and initial bacteria loads. Furthermore, the ROC analyses showed that TRIM4, 27 and 65 all exhibited the highest areas under the curve (AUC) values of 1.00 in discriminating active TB from LTBI and HCs. Moreover, TRIM27 combined with TRIM32 for an improved AUC value of 0.81 in discriminating LTBI from HCs. These results suggest that TRIM gene dysregulation might be involved in the pathogenesis of TB and that these genes could serve as potential biomarkers for indicating TB status. [ABSTRACT FROM AUTHOR]

Human rhythmic activities are variable. Cycle-to-cycle fluctuations form the behavioral observable. Traditional analysis focuses on statistical measures such as mean and variance. In this article we show that, by treating the fluctuations as a time series, one can apply techniques such as power spectra and rescaled range analysis to gain insight into the mechanisms underlying the remarkable abilities of humans to perform a variety of rhythmic movements, from maintaining memorized temporal patterns to anticipating and timing their movements to predictable sensory stimuli. [Copyright &y& Elsevier]